Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,813,080	G→A	P155P (CCG→CCA)	emrB →	multidrug efflux pump membrane subunit EmrB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,813,080	0	G	A	100.0%	177.2 / NA	51	P155P (CCG→CCA)	emrB	multidrug efflux pump membrane subunit EmrB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (18/33);  total (18/33)

ATTCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGAC‑GTGAAACCCGCAC  >  NC_000913/2812946‑2813202                                                                                                                                       |                                                                                                                            aTTCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCt                                                                                                                        >  1:1073455/1‑140 (MQ=255)   tCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCtag                                                                                                                      <  2:760186/140‑3 (MQ=255)   tCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTcg                                                                                                                      <  2:1073455/140‑1 (MQ=255)   tCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTcg                                                                                                                      <  2:1016855/140‑1 (MQ=255)   tCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTcg                                                                                                                      <  1:899089/140‑1 (MQ=255)          aTTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTta                                                                                                               >  2:1151709/1‑140 (MQ=255)            tGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGTGGCCCAATCCTCGGCGGTtata                                                                                                             >  1:939031/1‑140 (MQ=255)            tGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTtata                                                                                                             >  1:860257/1‑140 (MQ=255)            tGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTtata                                                                                                             >  2:1150764/1‑140 (MQ=255)            tGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTtata                                                                                                             >  1:767929/1‑140 (MQ=255)               cGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCa                                                                                                          <  2:128471/140‑1 (MQ=255)                gCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAg                                                                                                         <  1:580603/140‑1 (MQ=255)                  cGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCg                                                                                                       <  2:767929/140‑1 (MQ=255)                  cGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCg                                                                                                       <  2:41833/140‑1 (MQ=255)                  cGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCg                                                                                                       <  1:287994/140‑1 (MQ=255)                   gggCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGa                                                                                                      >  2:389861/1‑140 (MQ=255)                   gggCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGa                                                                                                      <  2:315818/140‑1 (MQ=255)                     gCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAg                                                                                                         >  2:1097744/1‑135 (MQ=255)                     gCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAg                                                                                                         <  1:1097744/135‑1 (MQ=255)                             tCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCAc                                                                                            <  1:70344/140‑1 (MQ=255)                             tCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCAc                                                                                            <  1:389861/140‑1 (MQ=255)                              cccGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACt                                                                                           <  1:1177071/140‑1 (MQ=255)                               ccGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTg                                                                                          >  1:638366/1‑140 (MQ=255)                               ccGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTg                                                                                          <  2:978062/140‑1 (MQ=255)                                  cTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTgggg                                                                                       <  2:211602/140‑1 (MQ=255)                                  cTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTgggg                                                                                       <  1:296698/140‑1 (MQ=255)                                  cTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTgggg                                                                                       <  2:802289/140‑1 (MQ=255)                                  cTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTgggg                                                                                       <  2:1115688/140‑1 (MQ=255)                                   tttCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGc                                                                                      <  1:980445/140‑1 (MQ=255)                                       gCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGa                                                                                  <  1:1181107/140‑1 (MQ=255)                                       gCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGa                                                                                  <  1:391232/140‑1 (MQ=255)                                       gCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGa                                                                                  <  1:67450/140‑1 (MQ=255)                                                aTTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTtca                                                                         <  2:337804/140‑1 (MQ=255)                                                       aaTAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGTGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGt                                                                  <  2:939031/140‑1 (MQ=255)                                                       aaTAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTTTATTCGCGAGAATTACCACAGGGGGTGGATATTCTTTCTTAACGt                                                                  >  2:1015577/1‑140 (MQ=255)                                                       aaTAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATTAACgg                                                                  >  2:9878/1‑139 (MQ=255)                                                       aaTAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGt                                                                  <  1:613968/140‑1 (MQ=255)                                                       aaTAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGt                                                                  >  1:85003/1‑140 (MQ=255)                                                            cTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGa                                                             >  2:129843/1‑140 (MQ=255)                                                             tACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGAt                                                            <  1:1151709/140‑1 (MQ=255)                                                                       ccaAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATtggcgtggcg                                                  >  2:187888/1‑140 (MQ=255)                                                                       ccaAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATtggcgtggcg                                                  >  1:1060630/1‑140 (MQ=255)                                                                       ccaAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATtggcgtggcg                                                  <  1:1150764/140‑1 (MQ=255)                                                                        caAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCgg                                                 <  1:829146/140‑1 (MQ=255)                                                                        caAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCgg                                                 <  2:85003/140‑1 (MQ=255)                                                                          aaCGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCggtg                                               <  2:388971/140‑1 (MQ=255)                                                                                cGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGGGGGGTtg                                         >  1:532410/1‑140 (MQ=255)                                                                                     gcgcTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGac                                    <  1:603810/140‑1 (MQ=255)                                                                                                           cGGTGATTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGGGGGGGTGGTGAAACCGCAAACCCTTCGCGGGC‑gg             >  2:1044012/1‑139 (MQ=255)                                                                                                                aTTGTCGCGCCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGAC‑GTGAAAc        <  1:246151/140‑1 (MQ=255)                                                                                                                       cgcCAATTTGCGGCCCAATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGGGGGGGTTGTGAAACTGCCAAAACTTCGCGGGCGGGGAAAccccccc  >  2:246151/1‑136 (MQ=255)                                                                                                                                       |                                                                                                                            ATTCAGGGGATTGTCGCCGGGCCGTTGATCCCGCTTTCGCAAAGTCTATTGCTGAATAACTACCCGCCAGCCAAACGCTCGATCGCGCTGGCGTTGTGGTCGATGACGGTGATTGTCGCGCCAATTTGCGGCCCGATCCTCGGCGGTTATATCAGCGATAATTACCACTGGGGCTGGATATTCTTCATCAACGTGCCGATTGGCGTGGCGGTGGTGTTGATGACACTGCAAACTCTGCGCGGAC‑GTGAAACCCGCAC  >  NC_000913/2812946‑2813202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑