BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	375,176	A→G	intergenic (+295/‑283)	mhpE → / → mhpT	4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	375,176	0	A	G	100.0%	126.7 / NA	41	intergenic (+295/‑283)	mhpE/mhpT	4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (20/21); total (20/21)

GCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCG  >  NC_000913/375041‑375311

gcgTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAAt                                                                                                                                     >  2:941649/1‑140 (MQ=37)

     aaCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTa                                                                                                                                >  2:230424/1‑140 (MQ=37)

      aCGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAg                                                                                                                               <  2:628545/140‑1 (MQ=37)

        gCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGc                                                                                                                             <  1:1011504/140‑1 (MQ=37)

        gCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGc                                                                                                                             <  2:294121/140‑1 (MQ=37)

                cGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAg                                                                                                                     <  1:1035817/140‑1 (MQ=37)

                cGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAg                                                                                                                     <  2:547819/140‑1 (MQ=37)

                  gCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGAc                                                                                                                   <  1:757133/140‑1 (MQ=37)

                   ccTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGAcg                                                                                                                  >  2:687561/1‑140 (MQ=255)

                       cGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGtt                                                                                                              >  1:189409/1‑140 (MQ=255)

                            ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                         >  2:840228/1‑140 (MQ=25)

                            ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt                                                                                                         <  1:940252/140‑1 (MQ=18)

                              cgcgGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCg                                                                                                       <  2:189409/140‑1 (MQ=18)

                                  ggAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATc                                                                                                   >  2:4993/1‑140 (MQ=18)

                                        tGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt                                                                                             <  1:1057779/140‑1 (MQ=17)

                                        tGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCAt                                                                                             <  1:155657/140‑1 (MQ=17)

                                             gCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTgcg                                                                                        <  1:230424/140‑1 (MQ=14)

                                                                   cGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTa                                                                  >  1:926177/1‑140 (MQ=11)

                                                                        cAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGc                                                             <  2:800027/140‑1 (MQ=18)

                                                                              tCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATc                                                       <  1:840228/140‑1 (MQ=18)

                                                                                     aCGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTa                                                >  2:593785/1‑140 (MQ=17)

                                                                                          tGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGAt                                           >  2:214047/1‑140 (MQ=18)

                                                                                            ccGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATgg                                         >  2:948632/1‑140 (MQ=21)

                                                                                              ggATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGgcg                                       <  2:926177/140‑1 (MQ=37)

                                                                                                aTGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGcgcg                                     <  2:725394/140‑1 (MQ=37)

                                                                                                 tGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCgg                                    >  2:767390/1‑140 (MQ=25)

                                                                                                   cggcgTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                  <  1:4993/140‑1 (MQ=38)

                                                                                                   cggcgTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGa                                  <  2:1165892/140‑1 (MQ=38)

                                                                                                   cggcgTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCCGCCTACGGATGGCGCGGGa                                  >  2:825817/1‑140 (MQ=255)

                                                                                                      cgTGAACGCCTTATCCGGCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAAtt                               >  2:81318/1‑140 (MQ=37)

                                                                                                                       gCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAc              >  1:496286/1‑140 (MQ=37)

                                                                                                                       gCCTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGAc              >  2:830453/1‑140 (MQ=37)

                                                                                                                        ccTACGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGTCCTGATAAGAcg             >  2:61906/1‑140 (MQ=255)

                                                                                                                           aCGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGt          >  2:642241/1‑140 (MQ=37)

                                                                                                                            cGGGTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGtt         >  1:24706/1‑140 (MQ=37)

                                                                                                                               gTGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGc      <  1:941649/140‑1 (MQ=37)

                                                                                                                                tGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCg     <  1:948632/140‑1 (MQ=25)

                                                                                                                                 ggCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGt    <  2:129026/140‑1 (MQ=25)

                                                                                                                                  gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTc   >  1:933506/1‑140 (MQ=18)

                                                                                                                                  gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTc   >  2:564867/1‑140 (MQ=18)

                                                                                                                                  gcgcGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTc   >  1:536499/1‑140 (MQ=18)

                                                                                                                                   cgcgGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCg  <  2:496286/140‑1 (MQ=25)

GCGTGAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACGGGTGGCGCGAGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCGCATCAGGCATCTGCGCACGACTGCCGGATGCGGCGTAAACGCCTTATCCGGCCTACGGATGGCGCGGGAATTTGTAGGCCTGATAAGACGCGTTAGCGTCG  >  NC_000913/375041‑375311

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`