Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	889,923	A→G	intergenic (‑104/‑166)	ybjL ← / → ybjM	putative transport protein YbjL/putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	889,923	0	A	G	100.0%	244.5 / NA	61	intergenic (‑104/‑166)	ybjL/ybjM	putative transport protein YbjL/putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (26/35);  total (26/35)

CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAAT  >  NC_000913/889788‑890056                                                                                                                                        |                                                                                                                                      ccATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGAt                                                                                                                                   <  2:253675/140‑1 (MQ=255)   aTTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATag                                                                                                                                 <  2:781962/140‑1 (MQ=255)   aTTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATag                                                                                                                                 <  2:305851/140‑1 (MQ=255)     ttAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagta                                                                                                                               <  2:166459/140‑1 (MQ=255)        aCAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaag                                                                                                                            <  1:724207/140‑1 (MQ=255)        aCAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaag                                                                                                                            <  1:332796/140‑1 (MQ=255)         cAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                           <  2:791375/140‑1 (MQ=255)            ttCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTAAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAAc                                                                                                                        >  1:427092/1‑140 (MQ=255)                      tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                           <  1:1028655/127‑1 (MQ=255)                      tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATagtaaagt                                                                                                                           >  2:1028655/1‑127 (MQ=255)                      tttATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATAtttt                                                                                                              >  1:609848/1‑140 (MQ=255)                        tataTTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTAc                                                                                                            <  2:769852/140‑1 (MQ=255)                         atatTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACt                                                                                                           <  2:277660/140‑1 (MQ=255)                              cACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCTGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                             >  2:524327/1‑133 (MQ=255)                              cACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCTGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTa                                                                                                             <  1:524327/133‑1 (MQ=255)                              cACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAgtgt                                                                                                      <  2:791477/140‑1 (MQ=255)                               aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                     >  2:202321/1‑140 (MQ=255)                               aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                     <  2:95936/140‑1 (MQ=255)                               aCGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTa                                                                                                     >  1:1042567/1‑140 (MQ=255)                                cGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                    <  1:33778/140‑1 (MQ=255)                                cGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTaa                                                                                                    >  1:717460/1‑140 (MQ=255)                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                   >  2:691742/1‑140 (MQ=255)                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                   <  2:884884/140‑1 (MQ=255)                                 ggAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAAt                                                                                                   >  2:175220/1‑140 (MQ=255)                                  gAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATc                                                                                                  <  2:1042567/140‑1 (MQ=255)                                     gCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATcaca                                                                                               <  1:995529/140‑1 (MQ=255)                                      cTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACAt                                                                                              >  1:613401/1‑140 (MQ=255)                                         aCTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAg                                                                                           <  1:1116537/140‑1 (MQ=255)                                                gTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCaa                                                                                    <  2:1167457/140‑1 (MQ=255)                                                gTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCaa                                                                                    >  2:942518/1‑140 (MQ=255)                                                         tAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTatat                                                                           >  1:252663/1‑140 (MQ=255)                                                          aaGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATGGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATg                                                                          <  2:725091/140‑1 (MQ=255)                                                           aGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGa                                                                         >  2:609790/1‑140 (MQ=255)                                                               gTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTg                                                                     >  1:912231/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                    >  1:32880/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                    >  2:714189/1‑140 (MQ=255)                                                                ttGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGc                                                                    >  1:786562/1‑140 (MQ=255)                                                                 tGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                   <  1:267490/140‑1 (MQ=255)                                                                 tGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCg                                                                   <  1:1130995/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                  >  1:321545/1‑140 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                  <  1:175220/140‑1 (MQ=255)                                                                  gaaagaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTAAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGt                                                                  <  2:427092/140‑1 (MQ=255)                                                                     agaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTgg                                                               <  2:717460/140‑1 (MQ=255)                                                                      gaaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                              <  2:1044077/140‑1 (MQ=255)                                                                       aaaTGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGcc                                                             <  2:912231/140‑1 (MQ=255)                                                                          tGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                              >  1:1023105/1‑136 (MQ=255)                                                                          tGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGc                                                              <  2:1023105/136‑1 (MQ=255)                                                                          tGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtat                                                          >  2:49910/1‑140 (MQ=255)                                                                          tGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCtat                                                          >  1:443520/1‑140 (MQ=255)                                                                                tttACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGc                                                    <  2:613401/140‑1 (MQ=255)                                                                                 ttACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCa                                                   >  1:877345/1‑140 (MQ=255)                                                                                      aTAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGaa                                              >  1:67030/1‑140 (MQ=255)                                                                                        aaTGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATg                                            >  1:1167690/1‑140 (MQ=255)                                                                                        aaTGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATg                                            <  1:49910/140‑1 (MQ=255)                                                                                            ttttttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGaa                                        <  1:815783/140‑1 (MQ=255)                                                                                              ttttACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGc                                      <  1:942518/140‑1 (MQ=255)                                                                                                   ccAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAGGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCtt                                 <  1:856192/140‑1 (MQ=255)                                                                                                          tCAGGGCGCAGATTCATTCAGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATTGAATGCGAAGCGGCTTTATCTTa                          <  2:321545/140‑1 (MQ=255)                                                                                                                      ttcattcaGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCcaca              <  1:714189/140‑1 (MQ=255)                                                                                                                             aGCGCACCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGa       >  1:801934/1‑140 (MQ=255)                                                                                                                                  aCCTAAGCGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAAt  >  2:81408/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                      CCATTTAACAATTCGGCGACGTTTATATTCACGGAGGCTAACTTCTTGTTTACTAGTAAGCTGTTGAAAGAAATGGTAATTTACGATAATGTTTTTTACCAGAATTCAGGGCGCAGATTCATTCAGCGCACCTAAACGATAGTAAAGTAACAATATATTTTACTAGTGTAATCACATTAGGTATCAACGGCTATATGAATTGCGTTGGCCTATATTAGCATGGAATGCGAAGCGGCTTTATCTTACTGAACGCCACACTGGCGAAAAAT  >  NC_000913/889788‑890056

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑