Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,935,488	C→T	R276H (CGC→CAC)	zwf ←	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,935,488	0	C	T	100.0%	169.6 / NA	49	R276H (CGC→CAC)	zwf	NADP(+)‑dependent glucose‑6‑phosphate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/25);  total (24/25)

ACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGCGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGC  >  NC_000913/1935353‑1935619                                                                                                                                        |                                                                                                                                    aCGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTc                                                                                                                                 >  2:1083049/1‑140 (MQ=255)    cgGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGAt                                                                                                                              <  1:308065/140‑1 (MQ=255)      gaattccACGAAAGTTTCTGTATTGCGGCTCTTGTTAGCGCCCTCTTCTTCCTAATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATgc                                                                                                                            >  2:275644/6‑140 (MQ=255)       aTCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATgcg                                                                                                                           >  1:69520/1‑140 (MQ=255)       aTCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATgcg                                                                                                                           <  2:711511/140‑1 (MQ=255)         cGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATgcggc                                                                                                                         <  1:908340/140‑1 (MQ=255)              cGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCaga                                                                                                                    <  1:542959/140‑1 (MQ=255)                            ttGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACttt                                                                                                      <  2:980300/140‑1 (MQ=255)                             tgctgcTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCaga                                                                                                                    >  1:203491/1‑125 (MQ=255)                             tgctgcTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCaga                                                                                                                    <  2:203491/125‑1 (MQ=255)                              gctgctCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCa                                                                                                    >  2:245754/1‑140 (MQ=255)                               ctgctCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCAc                                                                                                   >  2:929455/1‑140 (MQ=255)                               ctgctCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCAc                                                                                                   >  1:68445/1‑140 (MQ=255)                                  ctctTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACttt                                                                                                >  1:352212/1‑140 (MQ=255)                                  ctctTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACttt                                                                                                >  2:867214/1‑140 (MQ=255)                                    ctTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACttttt                                                                                              >  2:826039/1‑140 (MQ=255)                                       gTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCAt                                                                                           <  1:867214/140‑1 (MQ=255)                                         tCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATcg                                                                                         <  1:1159878/140‑1 (MQ=255)                                                ctctTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGc                                                                                  <  1:1083049/140‑1 (MQ=255)                                                     cttcCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCt                                                                             >  1:173812/1‑140 (MQ=255)                                                          aGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATTGCGGATGCTGTCTGCGCt                                                                        <  1:233797/140‑1 (MQ=255)                                                           gATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGTGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTc                                                                       >  2:406946/1‑140 (MQ=255)                                                           gATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTc                                                                       >  1:870412/1‑140 (MQ=255)                                                           gATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTc                                                                       >  1:777155/1‑140 (MQ=255)                                                                  cGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAg                                                                <  2:777155/140‑1 (MQ=255)                                                                       cTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGAcggc                                                           <  1:929455/140‑1 (MQ=255)                                                                                    ggCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGga                                                        >  2:111914/1‑130 (MQ=255)                                                                                    ggCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGga                                                        <  1:111914/130‑1 (MQ=255)                                                                                    ggCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCaa                                              >  1:815798/1‑140 (MQ=255)                                                                                    ggCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCaa                                              >  1:604601/1‑140 (MQ=255)                                                                                         aGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATg                                         >  1:249238/1‑140 (MQ=255)                                                                                            ccGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCaa                                      <  1:245754/140‑1 (MQ=255)                                                                                                        gCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTgcagc                          <  1:1116750/140‑1 (MQ=255)                                                                                                        gCCCGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTgcagc                          >  1:415517/1‑140 (MQ=255)                                                                                                         cccGCGTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTgcagca                         >  2:1095197/1‑140 (MQ=255)                                                                                                           cgcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  1:1095197/140‑1 (MQ=255)                                                                                                           cgcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  2:758048/140‑1 (MQ=255)                                                                                                           cgcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  2:452723/140‑1 (MQ=255)                                                                                                           cgcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  2:452424/140‑1 (MQ=255)                                                                                                           cgcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  1:343816/140‑1 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTTCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAggt                      >  2:472344/1‑140 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAggt                      >  2:130610/1‑140 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       >  2:436005/1‑139 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       >  2:463076/1‑139 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       >  2:662656/1‑139 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  1:436005/139‑1 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       >  2:192433/1‑139 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  1:192433/139‑1 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  1:463076/139‑1 (MQ=255)                                                                                                            gcgTACGGTTTTTTCGCGTACGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAgg                       <  1:662656/139‑1 (MQ=255)                                                                                                                                cGTTGGAGTGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCgcgc  <  2:415517/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                    ACGCGGATCGCCACGAAAGTTTCTGTATTGCTGCTCTTGTTCGCGCCCTCTTCTTCCAGATATCCCGGCACTTTTTTGCCCTGGGCGAAGCCCGCAGTATATTGCCCGCGTACGGTTTTTTCGCGTACGTTGGAGCGGTCGATGCGGCGCAGAGACTTCAGTACTTTCACTTTTTCATCGCGGATGCTGTCTGCGCTCAGGTCAGACGGCGGAGACATCGCAATCATGCAAAGAATTTGCAGCAGGTGGTTCTGGATCATGTCGCGC  >  NC_000913/1935353‑1935619

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑