Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,811,940	G→C	A172P (GCG→CCG)	emrA →	multidrug efflux pump membrane fusion protein EmrA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,811,940	0	G	C	100.0%	122.2 / NA	32	A172P (GCG→CCG)	emrA	multidrug efflux pump membrane fusion protein EmrA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (13/19);  total (13/19)

GCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCG  >  NC_000913/2811805‑2812076                                                                                                                                        |                                                                                                                                         gCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCa                                                                                                                                      >  1:348697/1‑140 (MQ=255)     atatTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTg                                                                                                                                  >  2:742331/1‑140 (MQ=255)               gCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGa                                                                                                                        <  1:200258/140‑1 (MQ=255)               gCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGa                                                                                                                        <  2:366694/140‑1 (MQ=255)                cAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGa                                                                                                                        >  1:157078/1‑139 (MQ=255)                cAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGa                                                                                                                        <  2:157078/139‑1 (MQ=255)                cAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGAt                                                                                                                       >  2:90916/1‑140 (MQ=255)                    aaaTCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTcaa                                                                                                                   <  1:167117/140‑1 (MQ=255)                    aaaTCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTcaa                                                                                                                   <  1:662962/140‑1 (MQ=255)                     aaTCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTcaac                                                                                                                  <  1:181268/140‑1 (MQ=255)                         gCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAacaata                                                                                                              <  1:534873/140‑1 (MQ=255)                            cTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAacaatacaa                                                                                                           <  1:338757/140‑1 (MQ=255)                             tCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAacaatacaat                                                                                                          >  2:371425/1‑140 (MQ=255)                                          aaGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCga                                                                                             <  1:439881/140‑1 (MQ=255)                                                       cgccgTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGAc                                                                                <  1:301853/140‑1 (MQ=255)                                                        gccgTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACt                                                                               <  1:90916/140‑1 (MQ=255)                                                                        cAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATcagc                                                               <  1:36270/140‑1 (MQ=255)                                                                        cAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATcagc                                                               >  2:342567/1‑140 (MQ=255)                                                                         aaTGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATcagcc                                                              <  1:523141/140‑1 (MQ=255)                                                                         aaTGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATcagcc                                                              <  2:583037/140‑1 (MQ=255)                                                                                     aTTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACa                                                  >  2:479660/1‑140 (MQ=255)                                                                                             cgAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCa                                          <  2:540418/140‑1 (MQ=255)                                                                                                 gagCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGa                                      <  1:342567/140‑1 (MQ=255)                                                                                                            cGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACg                           >  1:592505/1‑140 (MQ=255)                                                                                                            cGCCCGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACg                           >  1:467167/1‑140 (MQ=255)                                                                                                               ccGCGACGCCGTCACCAGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCct                        >  1:734751/1‑140 (MQ=255)                                                                                                                               aGTGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCg        >  1:675632/1‑140 (MQ=255)                                                                                                                                 tGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTa      <  1:742331/140‑1 (MQ=255)                                                                                                                                 tGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTa      <  1:371425/140‑1 (MQ=255)                                                                                                                                 tGCCCAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTa      >  1:478211/1‑140 (MQ=255)                                                                                                                                    ccAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTc   >  2:253326/1‑140 (MQ=255)                                                                                                                                     cAGCCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCg  <  1:400079/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         GCGAATATTGAGGTGCAGAAAATCGCCCTCGCGAAAGCACAAAGCGACTACAACCGCCGTGTGCCGCTGGGCAATGCCAACCTGATTGGTCGCGAAGAGCTGCAACACGCCCGCGACGCCGTCACCAGTGCCCAGGCGCAACTGGACGTCGCGATTCAACAATACAATGCCAATCAGGCGATGATTCTGGGGACTAAACTGGAAGATCAGCCAGCCGTGCAACAGGCTGCCACCGAAGTACGTAACGCCTGGCTGGCGCTGGAGCGTACTCG  >  NC_000913/2811805‑2812076

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑