Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,396,154	G→A	A35A (GCC→GCT)	yhdP ←	AsmA2 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,396,154	0	G	A	100.0%	152.3 / NA	45	A35A (GCC→GCT)	yhdP	AsmA2 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/25);  total (20/25)

CTTTTAGTTCTGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAGGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCT  >  NC_000913/3396024‑3396277                                                                                                                                   |                                                                                                                            cTTTTAGTTCTGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGa                                                                                                                    <  2:718172/140‑1 (MQ=255)   tttAGTTCTGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATg                                                                                                                  <  2:78103/140‑1 (MQ=255)      aGTTCTGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCgg                                                                                                               <  1:206746/140‑1 (MQ=255)        ttCTGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTa                                                                                                             <  1:448952/140‑1 (MQ=255)           tGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAg                                                                                                          <  1:375379/140‑1 (MQ=255)            gCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGGCGCCAAGCGTCAAGATGCGGTAAAGc                                                                                                         >  2:365880/1‑140 (MQ=255)            gCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGc                                                                                                         >  2:436036/1‑140 (MQ=255)                ggATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATa                                                                                                     <  1:177315/140‑1 (MQ=255)                  aTGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACg                                                                                                   >  2:718983/1‑140 (MQ=255)                    gTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCa                                                                                                 <  1:198550/140‑1 (MQ=255)                    gTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCa                                                                                                 <  2:648130/140‑1 (MQ=255)                     tCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAg                                                                                                >  2:408816/1‑140 (MQ=255)                                 aGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACcag                                                                                    <  1:365880/140‑1 (MQ=255)                                 aGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACcag                                                                                    >  1:21253/1‑140 (MQ=255)                                     tCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCg                                                                                            >  1:178269/1‑128 (MQ=255)                                     tCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCg                                                                                            <  2:178269/128‑1 (MQ=255)                                     tCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAgg                                                                                >  2:684363/1‑140 (MQ=255)                                     tCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAgg                                                                                <  2:214351/140‑1 (MQ=255)                                        ggCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGcc                                                                             >  2:552470/1‑139 (MQ=255)                                         gCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAg                                                                            <  2:452555/140‑1 (MQ=255)                                         gCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAg                                                                            <  1:6706/140‑1 (MQ=255)                                            aaaaTTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGa                                                                         >  1:456669/1‑140 (MQ=255)                                                     ccAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGa                                                                >  1:417386/1‑140 (MQ=255)                                                        gctggctgAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAgcg                                                             <  1:368279/140‑1 (MQ=255)                                                        gctggctgAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAgcg                                                             <  2:404138/140‑1 (MQ=255)                                                        gctggctgAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAgcg                                                             <  1:436036/140‑1 (MQ=255)                                                                agagCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCa                                                     <  2:64463/140‑1 (MQ=255)                                                                      gACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGc                                               >  2:251544/1‑140 (MQ=255)                                                                       aCTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCa                                              >  2:667594/1‑140 (MQ=255)                                                                          ggCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTa                                           >  1:552459/1‑140 (MQ=255)                                                                               cTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATc                                      >  1:638206/1‑140 (MQ=255)                                                                                 aCCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATccc                                    >  2:435847/1‑140 (MQ=255)                                                                                     gCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGc                                >  2:738917/1‑140 (MQ=255)                                                                                     gCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGc                                >  2:445825/1‑140 (MQ=255)                                                                                     gCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGc                                >  1:510817/1‑140 (MQ=255)                                                                                            aGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGcc                         <  2:421027/140‑1 (MQ=255)                                                                                              tCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTc                       <  1:421811/140‑1 (MQ=255)                                                                                               cgcgGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCa                      <  2:21253/140‑1 (MQ=255)                                                                                                gcgGATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCAc                     <  1:707380/140‑1 (MQ=255)                                                                                                  ggATTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCAccc                   <  2:417386/140‑1 (MQ=255)                                                                                                    aTTCTATTTTGTTGAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGt                 <  2:619191/140‑1 (MQ=255)                                                                                                            ttgttgAGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCtt         <  1:113354/140‑1 (MQ=255)                                                                                                                  aGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGc   >  1:275979/1‑140 (MQ=255)                                                                                                                  aGGATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGc   >  1:644154/1‑140 (MQ=255)                                                                                                                   ggATTTCCGGACGCCAAGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCt  <  2:638206/140‑1 (MQ=255)                                                                                                                                   |                                                                                                                            CTTTTAGTTCTGCACGGATGTCGTGTGCTTCAAGCGTCGGGCCAAAATTCTGCCAGCTGGCTGAGAGCTGACTGGCTTCTACCGGCATGCCAGTCGCGGATTCTATTTTGTTGAGGATTTCCGGACGCCAGGCGTCAAGATGCGGTAAAGCAATACGCAGGCCGCTAACCAGCAGGGCAGCGATCACAACGAGCGCGGCTCCAGTAAGCAGTAAAATCCCCGGCAATCGCCTCACCCGTCACTCCTTGTCTGCT  >  NC_000913/3396024‑3396277

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑