Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,299,250	A→T	G39G (GGT→GGA)	fdhF ←	formate dehydrogenase H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,299,250	0	A	T	100.0%	124.0 / NA	37	G39G (GGT→GGA)	fdhF	formate dehydrogenase H
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (19/18);  total (19/18)

TGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTA  >  NC_000913/4299118‑4299383                                                                                                                                     |                                                                                                                                      tGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCtggt                                                                                                                                >  2:181326/1‑140 (MQ=255) tGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCtggt                                                                                                                                <  1:720274/140‑1 (MQ=255)   ccTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCtggttg                                                                                                                              >  2:165407/1‑140 (MQ=255)      cATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTggttggtt                                                                                                                           <  2:539004/140‑1 (MQ=255)      cATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTggttggtt                                                                                                                           >  2:274345/1‑140 (MQ=255)       aTCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGttt                                                                                                                          >  1:656896/1‑140 (MQ=255)          ccAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTcc                                                                                                                       <  1:588675/140‑1 (MQ=255)              gAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTg                                                                                                                   >  2:338623/1‑140 (MQ=255)                aaCAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTgcg                                                                                                                 <  1:165407/140‑1 (MQ=255)                aaCAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTgcg                                                                                                                 <  2:660806/140‑1 (MQ=255)                aaCAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTgcg                                                                                                                 <  2:596822/140‑1 (MQ=255)                aaCAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTgcg                                                                                                                 <  1:184019/140‑1 (MQ=255)                             ttGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCg                                                                                                    <  2:7483/140‑1 (MQ=255)                                ccgccACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGAc                                                                                                 >  2:665115/1‑140 (MQ=255)                                                 gATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATcga                                                                                >  1:251778/1‑140 (MQ=255)                                                   tCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATcgacg                                                                              <  2:507020/140‑1 (MQ=255)                                                     aTGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCgacgac                                                                            >  2:486592/1‑140 (MQ=255)                                                         gggTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAgg                                                                        >  2:402129/1‑140 (MQ=255)                                                                     gcgGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCaa                                                            >  1:208172/1‑140 (MQ=255)                                                                      cgGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAAc                                                           <  1:665115/140‑1 (MQ=255)                                                                       ggggTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAAcc                                                          <  2:429650/140‑1 (MQ=255)                                                                        gggTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCt                                                         >  1:251240/1‑140 (MQ=255)                                                                         ggTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTg                                                        <  2:454457/140‑1 (MQ=255)                                                                              ggATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATgcg                                                   <  1:181326/140‑1 (MQ=255)                                                                                 tCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCaa                                                <  1:462318/140‑1 (MQ=255)                                                                                   tGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATa                                              <  2:720597/140‑1 (MQ=255)                                                                                     ggTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAgg                                            >  2:619414/1‑140 (MQ=255)                                                                                         tCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCa                                        >  1:667618/1‑140 (MQ=255)                                                                                               aTGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGt                                  >  2:19361/1‑140 (MQ=255)                                                                                                    gTCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTgacga                             >  2:380386/1‑140 (MQ=255)                                                                                                     tCCCAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTgacgac                            >  2:589486/1‑140 (MQ=255)                                                                                                       ccAGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACtt                          >  1:340304/1‑140 (MQ=255)                                                                                                         aGCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACtttt                        <  1:19361/140‑1 (MQ=255)                                                                                                          gCCATAATAACCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACttttt                       >  1:167844/1‑140 (MQ=255)                                                                                                                  aaCCCTTCAGACACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGt               <  1:149820/140‑1 (MQ=255)                                                                                                                  aaCCCTTCAGAAACAGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGt               <  2:550598/140‑1 (MQ=255)                                                                                                                               caGGGTTCCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTa  >  2:239643/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      TGCCTCATCCCAGGAAACAGGTTCGAGTTTGCCGCCACGCTGGCGACGGATCATGGGGGTTTTCAGGCGCGGGGTCAGGATCTGGGTATCGTTAATGAAGTCCCAGCCATAATAACCCTTCAGACACAGGGTACCCTGGTTGGTTTTCCCCTGCGCTGCCTCCGCCCGGACGATTTTGCCGTTATCGACGACCAGGTTGATTTTGCAACCTGATGCGCAATAGGGGCAAACCGTGACGACTTTTTTCATCGGTCTCGCTCCAGTTA  >  NC_000913/4299118‑4299383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑