Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,272,599	G→T	A31A (GCG→GCT)	chaC →	glutathione‑specific gamma‑glutamylcyclotransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,272,599	0	G	T	100.0%	139.9 / NA	40	A31A (GCG→GCT)	chaC	glutathione‑specific gamma‑glutamylcyclotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/20);  total (20/20)

ACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCG  >  NC_000913/1272462‑1272735                                                                                                                                          |                                                                                                                                         acCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGggctgc                                                                                                                                        >  2:593120/1‑140 (MQ=255)             tCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTggcggc                                                                                                                            >  2:710169/1‑140 (MQ=255)                         aTGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTg                                                                                                                <  2:88414/140‑1 (MQ=255)                               tGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGAcc                                                                                                          >  1:538213/1‑140 (MQ=255)                                 gCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCGTTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtg                                                                                                        >  2:116278/1‑140 (MQ=255)                                  cGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACTGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtga                                                                                                       >  2:292202/1‑140 (MQ=255)                                    gAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCtgatg                                                                                                     >  1:57036/1‑140 (MQ=255)                                      aGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAg                                                                                                   >  2:512541/1‑140 (MQ=255)                                         gtAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGa                                                                                                <  2:268349/140‑1 (MQ=255)                                         gtAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGa                                                                                                <  2:648870/140‑1 (MQ=255)                                          tAAGGTGATAACGCGTGATTTCTTGATGAATTCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAc                                                                                               <  1:118844/140‑1 (MQ=255)                                          tAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAc                                                                                               <  2:556780/140‑1 (MQ=255)                                          tAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAc                                                                                               <  1:324547/140‑1 (MQ=255)                                           aaGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAcc                                                                                              >  2:56350/1‑140 (MQ=255)                                               tGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCg                                                                                             <  2:37470/137‑1 (MQ=255)                                               tGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCg                                                                                             >  1:37470/1‑137 (MQ=255)                                               tGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgtg                                                                                          <  2:57036/140‑1 (MQ=255)                                               tGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGgtg                                                                                          >  2:354276/1‑140 (MQ=255)                                                 aTAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAc                                                                                               <  1:294699/133‑1 (MQ=255)                                                 aTAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGAc                                                                                               >  2:294699/1‑133 (MQ=255)                                                  tAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTgg                                                                                       >  2:61022/1‑140 (MQ=255)                                                      gcgTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCt                                                                                   <  1:593120/140‑1 (MQ=255)                                                       cgTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCtt                                                                                  <  1:710169/140‑1 (MQ=255)                                                       cgTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCtt                                                                                  <  2:349752/140‑1 (MQ=255)                                                         tGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCg                                                                                >  2:353423/1‑140 (MQ=255)                                                                ttGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATgg                                                                         >  2:684877/1‑140 (MQ=255)                                                                  gatgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGAt                                                                       <  2:688902/140‑1 (MQ=255)                                                                  gatgaATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGAt                                                                       <  1:509154/140‑1 (MQ=255)                                                                          ccGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAtg                                                               >  2:139713/1‑140 (MQ=255)                                                                          ccGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGAtg                                                               <  1:61022/140‑1 (MQ=255)                                                                             aTTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTgg                                                            >  1:57330/1‑140 (MQ=255)                                                                                 tAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATc                                                        <  2:57330/140‑1 (MQ=255)                                                                                                     ttGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGaa                                    <  1:599350/140‑1 (MQ=255)                                                                                                          gaaTCACTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTg                               <  1:354276/140‑1 (MQ=255)                                                                                                               aCTCTTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCg                          >  1:419115/1‑140 (MQ=255)                                                                                                                ctctTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCgg                         >  1:585194/1‑140 (MQ=255)                                                                                                                  ctTATGGTCAGCAGAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTa                       <  1:512541/140‑1 (MQ=255)                                                                                                                           agcagAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTg              >  2:589732/1‑140 (MQ=255)                                                                                                                           agcagAACAACGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTg              >  2:647238/1‑140 (MQ=255)                                                                                                                                 acaacGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGc        <  1:139713/140‑1 (MQ=255)                                                                                                                                  caacGGGCTGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCa       >  1:22483/1‑140 (MQ=255)                                                                                                                                       ggctgcTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATcgcg  >  1:73365/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                         ACCAAAAAGTTGTCGCAGGGAAGTATGCAGTGGCGGAAGTGTAAGGTGATAACGCGTGATTTCTTGATGAATGCCGATTGTAAAACGGCATTTGGTGCCATTGAAGAATCACTCTTATGGTCAGCAGAACAACGGGCGGCTTCGCTGGCGGCGACGCTGGCTTGTCGACCTGATGAGGGACCGGTGTGGATCTTCGGCTATGGATCGTTGATGTGGAATCCGGCACTGGAGTTTACCGAATCGTGCACCGGTACACTGGTTGGATGGCATCGCG  >  NC_000913/1272462‑1272735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑