Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,322,443	C→A	M21I (ATG→ATT)	secG ←	Sec translocon subunit SecG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,322,443	0	C	A	100.0%	48.2 / NA	15	M21I (ATG→ATT)	secG	Sec translocon subunit SecG
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (7/8);  total (7/8)

GATGATGAAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGT  >  NC_000913/3322308‑3322565                                                                                                                                        |                                                                                                                           gatgatgaAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGa                                                                                                                        <  2:155454/140‑1 (MQ=255)  atgatgaAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGAt                                                                                                                       >  1:44636/1‑140 (MQ=255)  atgatgaAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGAt                                                                                                                       >  2:317336/1‑140 (MQ=255)  atgatgaAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTACCATATCAGCGCCTTTACCTTGCTGCAGAATGAt                                                                                                                       >  1:319932/1‑140 (MQ=255)     atgaAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAg                                                                                                                    <  1:317336/140‑1 (MQ=255)      tgaAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGa                                                                                                                   <  1:213846/140‑1 (MQ=255)                   tGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCaa                                                                                                      <  1:12345/140‑1 (MQ=255)                         gagcGACGTTATGCGGGTCATGAAGTTACTAGAATCACTTGAACCAAACAGAGTAGCGGTAGCCCCTGCTCCGAAGGAGGTTCCAATATCAGCGCCTTTACCATGCTGCAGAATGATCAGTCAAGCAAGGCCAATTGcgt                                                                                                <  2:103069/139‑3 (MQ=255)                                   aTGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTACCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGaa                                                                                      <  2:319932/140‑1 (MQ=255)                                    tGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGaaa                                                                                     >  2:225163/1‑140 (MQ=255)                                                    aCCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCtt                                                                     <  1:44763/140‑1 (MQ=255)                                                      cAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTca                                                                   <  1:118289/140‑1 (MQ=255)                                                                                 cGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAg                                        <  1:223262/140‑1 (MQ=255)                                                                                    aaGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTg                                     >  1:90135/1‑140 (MQ=255)                                                                                      gcgcCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGcc                                   >  2:88399/1‑140 (MQ=255)                                                                                                                       ccTTTACCTTGCTGCAGAATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGt  >  1:41036/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                           GATGATGAAGAATAACGTTGCCAGCAGCGCCGTCATGCGGGTCATGAAGTTACCAGAACCACTTGAACCAAACAGCGTAGCGGAAGCGCCTGCTCCGAAGGAGGCTCCCATATCAGCGCCTTTACCTTGCTGCAGCATGATCAGACCAACAAGGCCAATTGCCACAATAAGGAAAACTACTAAAAGAGCTTCATACATAATCAACCTGTTCCTTGCGGAGTTGCCGCGTACCAATGCTTCAACCAATAAAGCGGGAGT  >  NC_000913/3322308‑3322565

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑