Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,333,582	(A)8→9	intergenic (‑131/‑128)	rplU ← / → ispB	50S ribosomal subunit protein L21/all‑trans‑octaprenyl‑diphosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,333,574	1	.	A	100.0%	109.3 / NA	32	intergenic (‑123/‑136)	rplU/ispB	50S ribosomal subunit protein L21/all‑trans‑octaprenyl‑diphosphate synthase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (15/17);  total (15/17)

CGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGC  >  NC_000913/3333445‑3333702                                                                                                                                   |                                                                                                                                 cgTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑aaaaaaaaac                                                                                                                        >  1:439906/1‑138 (MQ=255)      atatAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAAc                                                                                                                    >  1:555489/1‑140 (MQ=255)              cTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACgg                                                                                                            <  1:337173/140‑1 (MQ=255)                                   ttCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCAttt                                                                                       <  2:428238/140‑1 (MQ=255)                                       aTGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTc                                                                                   <  2:157055/140‑1 (MQ=255)                                                agTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc                                                                          <  1:104353/140‑1 (MQ=255)                                                agTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc                                                                          <  2:555489/140‑1 (MQ=255)                                                 gTGCACTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                                                                         <  2:220800/140‑1 (MQ=255)                                                  tGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAc                                                                        >  2:545403/1‑140 (MQ=255)                                                   gcgcTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc                                                                       <  2:439906/140‑1 (MQ=255)                                                   gcgcTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAcc                                                                       <  2:155348/140‑1 (MQ=255)                                                    cgcTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCAccc                                                                      >  1:472683/1‑140 (MQ=255)                                                      cTATAAATAATCTCAATAGGGCGCAAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCta                                                                    <  2:483315/140‑1 (MQ=255)                                                               ttCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc                                                                          <  2:46968/125‑1 (MQ=255)                                                               ttCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATc                                                                          >  1:46968/1‑125 (MQ=255)                                                                 cacaATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                                                                         >  1:376217/1‑124 (MQ=255)                                                                 cacaATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCa                                                                         <  2:376217/124‑1 (MQ=255)                                                                   caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                       <  2:378232/140‑1 (MQ=255)                                                                   caATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                       >  2:80967/1‑140 (MQ=255)                                                                        gggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                       <  2:449424/135‑1 (MQ=255)                                                                        gggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCAt                                                       >  1:449424/1‑135 (MQ=255)                                                                         ggCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAAccc                                                 >  1:216863/1‑140 (MQ=255)                                                                             cgAATATTACGCAAAACGCACGCCTTTAACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAg                                             >  1:113179/1‑140 (MQ=255)                                                                              gAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGt                                            <  2:216863/140‑1 (MQ=255)                                                                                        cAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                  <  1:545403/140‑1 (MQ=255)                                                                                          aaaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTca                                <  2:472683/140‑1 (MQ=255)                                                                                           aaCGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTcac                               >  2:479574/1‑140 (MQ=255)                                                                                             cgcacgcCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                  <  1:501632/135‑1 (MQ=255)                                                                                             cgcacgcCTTTGACAAGTGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGtt                                  >  2:501632/1‑135 (MQ=255)                                                                                                              tGCTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGa            >  1:503771/1‑140 (MQ=255)                                                                                                                cTACAGTCAATACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGaaa          >  1:152358/1‑140 (MQ=255)                                                                                                                        aaTACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGc  >  1:67647/1‑140 (MQ=255)                                                                                                                        aaTACACGAAGAAAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGc  >  1:149083/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                 CGTACATATAAAACTCCGCTTCCGCGCACACCTTTTCAATGATTCAGAGTGCGCTATAAATATTCACAATAGGGCGCGAATATTACGCAAAACGCACGCCTTTGACAAGTGCTACAGTCAATACACGAAG‑AAAAAAAACACAACTTGTACGGTAACGTTTATCTGTGCCATTTTTTCAGTACAATCACCCTATATTCCTAACCATAAACCCTAAGTTGCCTTTGTTCACAGTAAGGTAATCGGGGCGAAAAGCCCGGC  >  NC_000913/3333445‑3333702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑