Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,856,634	C→T	V99I (GTT→ATT)	ydjH ←	putative sugar kinase YdjH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,856,634	0	C	T	100.0%	107.9 / NA	32	V99I (GTT→ATT)	ydjH	putative sugar kinase YdjH
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/17);  total (15/17)

GAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGA  >  NC_000913/1856500‑1856761                                                                                                                                       |                                                                                                                                gAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTaa                                                                                                                            <  1:13530/140‑1 (MQ=255) gAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTaa                                                                                                                            <  1:189868/140‑1 (MQ=255)  aaagtACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAAt                                                                                                                           >  1:183798/5‑140 (MQ=255)   aaaTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATa                                                                                                                          <  2:525950/140‑1 (MQ=255)     aTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATaga                                                                                                                        <  2:556329/140‑1 (MQ=255)     aTACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATaga                                                                                                                        <  1:397885/140‑1 (MQ=255)       aCTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAgg                                                                                                                      >  1:436837/1‑140 (MQ=255)         tGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTa                                                                                                                    >  2:11233/1‑140 (MQ=255)                tGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGc                                                                                                             >  1:272760/1‑140 (MQ=255)                       aatTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTc                                                                                                      <  2:489899/140‑1 (MQ=255)                          ttCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTg                                                                                                   >  2:447550/1‑140 (MQ=255)                            cGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCt                                                                                                 >  1:534048/1‑140 (MQ=255)                              ccTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTc                                                                                               >  1:405540/1‑140 (MQ=255)                              ccTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTc                                                                                               <  2:183798/140‑1 (MQ=255)                                 gagaAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAgg                                                                                            <  2:272760/140‑1 (MQ=255)                                            gcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAat                                                                                    <  1:502308/137‑1 (MQ=255)                                            gcAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAat                                                                                    >  2:502308/1‑137 (MQ=255)                                             cAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAat                                                                                    >  1:51242/1‑136 (MQ=255)                                             cAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAat                                                                                    <  2:51242/136‑1 (MQ=255)                                                        tCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCGCCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCttt                                                                     <  2:475687/140‑1 (MQ=255)                                                            cAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCgg                                                                 <  2:534048/140‑1 (MQ=255)                                                                     aTTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCg                                                        >  2:454984/1‑140 (MQ=255)                                                                       ttCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCgag                                                      >  1:460615/1‑140 (MQ=255)                                                                                         cGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                      <  1:272867/138‑1 (MQ=255)                                                                                         cGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCg                                      >  2:272867/1‑138 (MQ=255)                                                                                           gTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                  <  1:402094/140‑1 (MQ=255)                                                                                           gTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCAt                                  >  2:402094/1‑140 (MQ=255)                                                                                                         tCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGa                    <  2:460615/140‑1 (MQ=255)                                                                                                         tCCGCTCGCCATCCTCCGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGa                    <  1:11233/140‑1 (MQ=255)                                                                                                                    tcctccGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGc         <  2:436837/140‑1 (MQ=255)                                                                                                                     cctccGTCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCt        >  1:5424/1‑140 (MQ=255)                                                                                                                           tCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGa  >  1:558321/1‑140 (MQ=255)                                                                                                                           tCACCAGTCCAATGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGa  <  2:558321/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                GAAAATACTGGCCAGTGATAATAATTTCGCCTGAGAAAACCGCGCAAAATCAACATCGTCAATATTCAATTTCCACAGACTGCCATTACGGTTTGTGACAAACGTCCGCTCGCCATCCTCCGTCACCAGTCCAACGTTAATAGAGGTATCTATGCTGACGTCCTGCTTCAGGCTTTGAATATCAATATTCTCTTTGCGGCAATGGTCGAGAATAAATTGCCCTGCGGCATCTTTACCAATACGACTCATTAATGCTGTGCGA  >  NC_000913/1856500‑1856761

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑