Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,883,982	G→C	W265C (TGG→TGC)	yeaV →	putative transporter YeaV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,883,982	0	G	C	100.0%	116.1 / NA	30	W265C (TGG→TGC)	yeaV	putative transporter YeaV
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (12/18);  total (12/18)

ACCGAATTTATTACCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGGTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGC  >  NC_000913/1883848‑1884117                                                                                                                                       |                                                                                                                                        aCCGAATTTATTACCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGAt                                                                                                                                    >  2:538636/1‑140 (MQ=255)  ccGAATTTATTACCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGAtc                                                                                                                                   <  2:392180/140‑1 (MQ=255)       tttATTACCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATa                                                                                                                              <  1:477655/140‑1 (MQ=255)          attaCCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATacac                                                                                                                           <  1:538636/140‑1 (MQ=255)             aCCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACAcccc                                                                                                                        <  1:200887/140‑1 (MQ=255)                aaCAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTgg                                                                                                                     <  2:613113/140‑1 (MQ=255)                     catcatCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAg                                                                                                                <  2:380911/140‑1 (MQ=255)                      atcatcAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGc                                                                                                               <  1:112709/140‑1 (MQ=255)                          tcaATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATg                                                                                                           >  1:559008/1‑140 (MQ=255)                             aTGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGttt                                                                                                        >  2:268344/1‑140 (MQ=255)                                      gCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCcgc                                                                                               >  2:96476/1‑140 (MQ=255)                                           aCCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTc                                                                                          >  1:232846/1‑140 (MQ=255)                                              aCGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCcg                                                                                       <  1:96476/140‑1 (MQ=255)                                              aCGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCcg                                                                                       <  1:59715/140‑1 (MQ=255)                                              aCGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCcg                                                                                       <  1:574102/140‑1 (MQ=255)                                                     aCTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGt                                                                                <  2:232846/140‑1 (MQ=255)                                                                  gAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAaagaag                                                                   <  2:433550/140‑1 (MQ=255)                                                                   aGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGTGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGt                                                                  <  1:269627/140‑1 (MQ=255)                                                                              cGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGAc                                                       >  1:526075/1‑140 (MQ=255)                                                                              cGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGAc                                                       >  2:248982/1‑140 (MQ=255)                                                                              cGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGAc                                                       <  2:559008/140‑1 (MQ=255)                                                                                          cGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGgca                                           <  2:526075/140‑1 (MQ=255)                                                                                               gTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCAcc                                      >  1:780035/1‑140 (MQ=255)                                                                                                          cGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGgcag                                          >  2:252169/1‑125 (MQ=255)                                                                                                          cGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGgcag                                          <  1:252169/125‑1 (MQ=255)                                                                                                          cGCAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTg                           >  1:731056/1‑140 (MQ=255)                                                                                                            cAACTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGt                         <  2:731056/140‑1 (MQ=255)                                                                                                             aaCTGGACCGTTTTCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGtt                        <  1:268344/140‑1 (MQ=255)                                                                                                                       ttttCTACTGGCTGTGCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGc              >  2:449275/1‑140 (MQ=255)                                                                                                                                   tgtgCTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGc  >  2:745794/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                        ACCGAATTTATTACCAACAGCATCATCAATGCCATCGGCCTGACCACGCAAAACTTCCTGCAAATGAGCTTATTCACCGATCCGCTTGGCGATGGTTCATTTACCCGCAACTGGACCGTTTTCTACTGGCTGTGGTGGATCTCATACACCCCTGGCGTAGCAATGTTTGTCACCCGCGTTTCCCGCGGTCGTAAGATTAAAGAAGTTATCTGGGGACTGATCCTCGGCAGCACCGTCGGTTGCTGGTTCTTCTTTGGCGTAATGGAAAGC  >  NC_000913/1883848‑1884117

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑