| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,145,107 | A→T | C12S (TGC→AGC) | hybA ← | hydrogenase 2 iron‑sulfur protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,145,107 | 0 | A | T | 97.8% | 140.0 / NA | 45 | C12S (TGC→AGC) | hybA | hydrogenase 2 iron‑sulfur protein |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base T (23/21); minor base C (1/0); total (24/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCG > NC_000913/3144972‑3145242 | acTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCtggag < 2:547068/140‑1 (MQ=255) ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGgctg > 1:505067/1‑140 (MQ=255) ggTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGgctg > 1:373010/1‑140 (MQ=255) gCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTtaa > 2:738207/1‑140 (MQ=255) gCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTtaa > 2:672773/1‑140 (MQ=255) cTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAAt < 1:132882/140‑1 (MQ=255) gCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTc > 2:418255/1‑140 (MQ=255) cTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCtt > 2:111051/1‑139 (MQ=255) cTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTg > 2:122336/1‑140 (MQ=255) cTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTg > 1:70610/1‑140 (MQ=255) tACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCCCCCGTCAGCAATGCCCCGCCGGGGGCTGCCTTAATAAAATTACGTCCGt > 2:509242/1‑140 (MQ=255) aCGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGtt < 1:403374/140‑1 (MQ=255) aCGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGtt < 2:373010/140‑1 (MQ=255) aaGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGt < 1:255897/140‑1 (MQ=255) ggTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTa > 2:679256/1‑140 (MQ=255) ggTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTa > 1:88175/1‑140 (MQ=255) cGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATtc > 2:695401/1‑140 (MQ=255) aGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATtctc < 1:679256/140‑1 (MQ=255) caacaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATtct > 1:125914/1‑132 (MQ=255) caacaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATtct < 2:125914/132‑1 (MQ=255) caacaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGa > 1:399207/1‑140 (MQ=255) caacaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGa > 1:512633/1‑140 (MQ=255) caacaTCCCCAGCGATCCCGGAATTGGCGGGCGGGTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGa > 2:235460/1‑140 (MQ=255) acaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGt < 2:512633/140‑1 (MQ=255) acaTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGt < 2:399207/140‑1 (MQ=255) caTCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTc > 1:764121/1‑140 (MQ=255) ccccAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGc < 1:695401/140‑1 (MQ=255) ccAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCCTGACTGACAGACCGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGt > 2:615547/1‑140 (MQ=255) aTCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtctt > 2:737660/1‑140 (MQ=255) cGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTAtctttctt < 2:70610/140‑1 (MQ=255) gAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCtttt < 2:476949/140‑1 (MQ=255) ggcgggcgGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTga < 1:672773/140‑1 (MQ=255) gcggTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGAcc < 1:122336/140‑1 (MQ=255) ccGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTgacgac > 2:796285/1‑125 (MQ=255) ccGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTgacgac < 1:796285/125‑1 (MQ=255) ctgacAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACg < 1:738207/140‑1 (MQ=255) cGGCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACAcc > 1:225406/1‑140 (MQ=255) cGGCAGCGCGCCCATCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACAcc > 1:589861/1‑140 (MQ=255) ggCAGCGCGCCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCg < 2:505067/140‑1 (MQ=255) gcgcCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGcaacc < 2:88175/140‑1 (MQ=255) gcCCGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGcaaccaa < 1:235460/140‑1 (MQ=255) gcCCATCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGcaaccaa < 2:589861/140‑1 (MQ=255) cGTCAGCAATGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAAccaaccc < 1:509242/140‑1 (MQ=255) tGCCCCGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACAcc > 2:210228/1‑140 (MQ=255) ccccGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACAccgc > 1:225149/1‑140 (MQ=255) ccGCTGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACAccgccg < 2:685507/140‑1 (MQ=255) | ACTTGGTGACGCAAGCCTGGCAGCCTACGCACAAGGTCGAGTCGTACAACATCCCCAGCGATCCCGGAATTGGCGGGCGGTTTTCAGCAGCCGCATGACTGACAGACGGCAGCGCGCCCGTCAGCAATGCCCCGCAGGAGGCTGCTTTAATAAAATTACGTCTGTTCACGGTTATTCTCCCCGTGAGTCAGCGTTATCTTTCTTTTGCTGACGACCCAGTTCACGCACCGCCATCACGCTGACACCGGCAACCAACCCAACCACACCGCCG > NC_000913/3144972‑3145242 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |