Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,970,191	T→A	V20E (GTG→GAG)	wecB →	UDP‑N‑acetylglucosamine 2‑epimerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,970,191	0	T	A	100.0%	141.9 / NA	41	V20E (GTG→GAG)	wecB	UDP‑N‑acetylglucosamine 2‑epimerase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (20/21);  total (20/21)

ACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACA  >  NC_000913/3970057‑3970326                                                                                                                                       |                                                                                                                                        aCCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATg                                                                                                                                    >  2:278339/1‑140 (MQ=255)     gccgTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGtt                                                                                                                                >  1:560297/1‑140 (MQ=255)                      cAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCttttt                                                                                                               >  2:709823/1‑140 (MQ=255)                         cacTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTg                                                                                                            <  1:709823/140‑1 (MQ=255)                                        cgcAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTAGTACGCGCCCGGAAGCCATCAAGATGGTGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCg                                                                                             >  2:632792/1‑140 (MQ=255)                                         gcAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGt                                                                                            <  2:499020/140‑1 (MQ=255)                                                cgcTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCTCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTgcg                                                                                     <  2:110399/140‑1 (MQ=255)                                                cgcTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTgcg                                                                                     <  1:251039/140‑1 (MQ=255)                                                cgcTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTgcg                                                                                     <  2:190412/140‑1 (MQ=255)                                                  cTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCg                                                                                             <  1:293712/130‑1 (MQ=255)                                                  cTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCg                                                                                             >  2:293712/1‑130 (MQ=255)                                                              tCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGAt                                                                       <  2:239874/140‑1 (MQ=255)                                                                 aaGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCt                                                                    >  2:3518/1‑140 (MQ=255)                                                                   gagaATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCg                                                                  <  1:56342/140‑1 (MQ=255)                                                                      aaTCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATc                                                               >  1:787524/1‑140 (MQ=255)                                                                         cGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAgg                                                            <  2:787524/140‑1 (MQ=255)                                                                           aTGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTg                                                          >  1:587511/1‑140 (MQ=255)                                                                                aaaGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCt                                                                    <  2:390995/125‑1 (MQ=255)                                                                                aaaGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCt                                                                    >  1:390995/1‑125 (MQ=255)                                                                                aaaGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGaa                                                     >  2:508484/1‑140 (MQ=255)                                                                                      ctgactgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCtt                                               <  1:180272/140‑1 (MQ=255)                                                                                       tgactgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCttt                                              >  1:272215/1‑140 (MQ=255)                                                                                       tgactgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCttt                                              >  2:36573/1‑140 (MQ=255)                                                                                           tgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCa                                          <  1:36573/140‑1 (MQ=255)                                                                                           tgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCa                                          <  2:609940/140‑1 (MQ=255)                                                                                           tgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCa                                          >  1:609940/1‑140 (MQ=255)                                                                                           tgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCa                                          <  1:470510/140‑1 (MQ=255)                                                                                           tgTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCa                                          >  1:581072/1‑140 (MQ=255)                                                                                              aTTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTg                                       >  1:598462/1‑140 (MQ=255)                                                                                              aTTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGAGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTg                                       >  2:219117/1‑140 (MQ=255)                                                                                                   gTACGGGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCt                                  >  2:193577/1‑140 (MQ=255)                                                                                                   gTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCt                                  >  1:717551/1‑140 (MQ=255)                                                                                                            cGGAAGCCATCAAGATGGCGCCGTTGGAGCATGAGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGAt                         <  1:219117/140‑1 (MQ=255)                                                                                                             ggAAGCCATCAAGATGGCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTATGAtc                        <  1:278339/140‑1 (MQ=255)                                                                                                                            ggtgcCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGc         <  1:632792/137‑1 (MQ=255)                                                                                                                            ggCGCCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGc         >  2:77850/1‑140 (MQ=255)                                                                                                                             gcgcCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGcc        >  2:206161/1‑140 (MQ=255)                                                                                                                             gcgcCGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGcc        <  1:3518/140‑1 (MQ=255)                                                                                                                                 cGTTGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGa    <  2:717551/140‑1 (MQ=255)                                                                                                                                   ttGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACa  <  1:508484/140‑1 (MQ=255)                                                                                                                                   ttGGAGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACa  <  2:581072/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                        ACCCGCCGTTGCTCGAAATAGCAACACTGCTGCGGTGAGCGCAAAGGCGCTCGCCGCTTATTCGAAGAGAATCGATGTGAAAGTACTGACTGTATTTGGTACGCGCCCGGAAGCCATCAAGATGGCGCCGTTGGTGCATGCGTTGGCAAAAGATCCTTTTTTTGAGGCTAAAGTTTGCGTCACTGCGCAGCATCGGGAGATGCTCGATCAGGTGCTGAAACTCTTTTCCATTGTACCTGACTACGATCTCAACATAATGCAGCCAGGACA  >  NC_000913/3970057‑3970326

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑