Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,020,937	T→C	intergenic (‑18/‑201)	sulA ← / → sxy	cell division inhibitor SulA/transcriptional coactivator for CRP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,020,937	0	T	C	100.0%	180.5 / NA	46	intergenic (‑18/‑201)	sulA/sxy	cell division inhibitor SulA/transcriptional coactivator for CRP
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/25);  total (21/25)

CGCGATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCC  >  NC_000913/1020802‑1021069                                                                                                                                        |                                                                                                                                     cgcgATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGt                                                                                                                                  >  1:768235/1‑140 (MQ=255) cgcgATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGt                                                                                                                                  <  2:703214/140‑1 (MQ=255) cgcgATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGt                                                                                                                                  >  1:414904/1‑140 (MQ=255) cgcgATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGt                                                                                                                                  <  2:207601/140‑1 (MQ=255)   cgATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTa                                                                                                                                <  1:218488/140‑1 (MQ=255)        gACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTa                                                                                                                           <  1:39576/140‑1 (MQ=255)         aCAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTAt                                                                                                                          <  2:543923/140‑1 (MQ=255)           aaCTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATgg                                                                                                                        <  1:781454/140‑1 (MQ=255)              ttCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATg                                                                                                                     <  2:11139/140‑1 (MQ=255)              ttCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATg                                                                                                                     <  2:240015/140‑1 (MQ=255)               tCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATgt                                                                                                                    <  2:414904/140‑1 (MQ=255)               tCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATgt                                                                                                                    <  2:393839/140‑1 (MQ=255)               tCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATgt                                                                                                                    <  2:768235/140‑1 (MQ=255)                          cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATg                                                                                                                     >  1:624432/1‑128 (MQ=255)                          cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATg                                                                                                                     <  2:624432/128‑1 (MQ=255)                          cccGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATcc                                                                                                         >  1:475230/1‑140 (MQ=255)                             ggCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGt                                                                                                      <  2:475230/140‑1 (MQ=255)                              gCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTg                                                                                                     <  1:589675/140‑1 (MQ=255)                               cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATg                                                                                                                     >  2:92151/1‑123 (MQ=255)                               cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATg                                                                                                                     <  1:92151/123‑1 (MQ=255)                               cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGa                                                                                                    <  2:295848/140‑1 (MQ=255)                               cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGa                                                                                                    <  1:509037/140‑1 (MQ=255)                               cTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGa                                                                                                    <  1:48156/140‑1 (MQ=255)                                        tttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGa                                                                                           >  2:168991/1‑140 (MQ=255)                                         ttCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGAt                                                                                          >  1:717885/1‑140 (MQ=255)                                           ccGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCa                                                                                        <  1:643298/140‑1 (MQ=255)                                            cGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGt                                                                                                      >  1:778071/1‑125 (MQ=255)                                            cGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGt                                                                                                      <  2:778071/125‑1 (MQ=255)                                            cGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCaa                                                                                       <  2:642282/140‑1 (MQ=255)                                                   acacGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTAtt                                                                                >  1:301812/1‑140 (MQ=255)                                                       gcgcAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCg                                                                            >  2:152255/1‑140 (MQ=255)                                                                       tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaaa                                                            >  1:162185/1‑140 (MQ=255)                                                                       tgcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCaaa                                                            >  2:323287/1‑140 (MQ=255)                                                                        gcGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAAt                                                           >  2:329843/1‑140 (MQ=255)                                                                                  acgacgAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTg                                                 <  1:13808/140‑1 (MQ=255)                                                                                     acgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTg                                              >  1:472383/1‑140 (MQ=255)                                                                                      cgaAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGa                                             <  2:301812/140‑1 (MQ=255)                                                                                               aTGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAAc                                    >  1:201796/1‑140 (MQ=255)                                                                                                            gAAGTGTACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCtt                       >  1:761106/1‑140 (MQ=255)                                                                                                                 gtACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGcccc                  <  2:761106/140‑1 (MQ=255)                                                                                                                  tACATAATCAATCCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCt                 >  1:766541/1‑140 (MQ=255)                                                                                                                            atcCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGc                     <  1:465687/126‑1 (MQ=255)                                                                                                                            atcCAGCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGCGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGc                     >  2:465687/1‑126 (MQ=255)                                                                                                                                 gCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGcc  >  2:513011/1‑140 (MQ=255)                                                                                                                                 gCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGcc  >  2:464091/1‑140 (MQ=255)                                                                                                                                 gCCCCTGCGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGcc  >  1:685967/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     CGCGATAGACAACTTCACTGATAAGCCCGGCTGTAGTGTTTTCCGTAGAGACACGCGCAATTTTACTTGCTGCGGATGAGAACGACGAAGAACGATGTGCATAGCCTGAAGTGTACATAATCAATCCAGCCCCTGTGAGTTACTGTATGGATGTACAGTACATCCAGTGACAACAAAGATCAACCCTATTTTCGGAAAGAGCCTCGCAAATTTTGTCGTTGGTGACGGGAAAACATAAATTAATCTTGCCCCTTAAGAATAAGTTGCC  >  NC_000913/1020802‑1021069

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑