Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913445,5580AC31.0% 37.0 / 15.9 29V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (17/3);  new base C (0/9);  total (17/12)
Fisher's exact test for biased strand distribution p-value = 2.20e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.69e-01
Rejected as consensus: Frequency below/above cutoff threshold.

AACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGA  >  NC_000913/445424‑445694
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aaCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATAccccc                                                                                                                                     <  1:691739/140‑1 (MQ=255)
        tttAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCCGCGGGAAAAACCCCCCCACCGTc                                                                                                                             >  2:523624/1‑140 (MQ=255)
             cGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACa                                                                                                                        <  1:168568/140‑1 (MQ=255)
                 tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAAAATGcc                                                                                                                    >  2:693944/1‑140 (MQ=255)
                             aTGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGGCAGCATTGCGCCCGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATTCAgcc                                                                                                        >  2:320278/1‑140 (MQ=255)
                                       ggttttAAATGCGCAACCTGCTGACATCCGGCGTTTCTTTCATGGTCCGGGCGCCTGCCACCCACACGGCGGCAAGCTTGGCCCCAGCGAAAAATCCCCCCTGACCGACAAACATGCCGTTAATCCAGCCGCCCAGCGaa                                                                                              <  2:569660/137‑1 (MQ=255)
                                        gATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTCCATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTTCGCCAGCGAGAAATACCCCCTGACCGTCAAACAAGCCGGTAATCCAGCCGCCCAGCGAAc                                                                                             >  2:353931/1‑140 (MQ=255)
                                                  cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                                   >  1:224065/1‑140 (MQ=255)
                                                   gcAAACTGCTGACATACGGCGGTTCTTTTATTGTACTGGGGACTCTCAGCCACACCGCGGCCCGCATTTCGCCAGCGGGAAAAAACCCCCCGCCGTCAAAAAAACCGCTAATCCAGCCGCCCCGCGAACCGCCCATcccc                                                                                  >  2:669161/1‑140 (MQ=255)
                                                                aTACGGCGGTTCTTTCATGGTACGGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAAct                                                                     <  1:523624/140‑1 (MQ=255)
                                                                   cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAAAACCCCCCGACCGTCAAACATGCCGTTAATCCAGCCCCCCAGCGAACCGCCAATCGCCACCCCAAAAAAatggc                                                                  >  2:579720/1‑140 (MQ=255)
                                                                       gtttttttCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                              <  2:228370/135‑1 (MQ=255)
                                                                       ggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                              <  1:320278/140‑1 (MQ=255)
                                                                                   gTACTGGCGACTGCCACCCACACTGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCGGACCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                                  <  2:474694/140‑1 (MQ=255)
                                                                                       tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATc                                              >  1:673438/1‑140 (MQ=255)
                                                                                         gcgcCTGTCAGCCACACGGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCGATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGc                                            <  1:693944/140‑1 (MQ=255)
                                                                                            ccTGCCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                         <  2:224065/139‑1 (MQ=255)
                                                                                            aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                         >  1:290156/1‑140 (MQ=255)
                                                                                            aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                         >  1:614369/1‑140 (MQ=255)
                                                                                            aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                         >  2:492997/1‑140 (MQ=255)
                                                                                            aCTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAAAAACCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGt                                         <  2:640108/140‑1 (MQ=255)
                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  2:64837/1‑140 (MQ=255)
                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  1:182744/1‑140 (MQ=255)
                                                                                              tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                       >  1:437855/1‑140 (MQ=255)
                                                                                                   gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCtttt                                  >  1:30432/1‑139 (MQ=255)
                                                                                                        acTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAcc                             >  1:636219/1‑140 (MQ=255)
                                                                                                          tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                           >  1:559157/1‑140 (MQ=255)
                                                                                                          tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                           >  1:340799/1‑140 (MQ=255)
                                                                                                            cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                         >  2:766861/1‑140 (MQ=255)
                                                                                                                cAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                     <  1:64837/140‑1 (MQ=255)
                                                                                                                cAGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCg                     <  1:100610/140‑1 (MQ=255)
                                                                                                                 aGCATTGCGCCGGCGAAAAATCCCCCCGCACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                    <  2:340799/140‑1 (MQ=255)
                                                                                                                 aGCATTGCGCCAGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                    <  1:579720/140‑1 (MQ=255)
                                                                                                                     ttGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct                <  2:437855/140‑1 (MQ=255)
                                                                                                                       gcgcCAGGGAAAAACCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGGGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  1:614073/140‑1 (MQ=255)
                                                                                                                       gcgcCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt              <  1:520092/140‑1 (MQ=255)
                                                                                                                       gccccggCGAAAAATCCCCCCTGCCCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTTTAACCTGCTGGCGACTCttt              <  2:30432/134‑1 (MQ=255)
                                                                                                                             gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt        >  1:257063/1‑140 (MQ=255)
                                                                                                                             gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt        >  1:162620/1‑140 (MQ=255)
                                                                                                                                 aaaaTCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt    <  1:211974/139‑1 (MQ=255)
                                                                                                                                 aaaaTCCCCCCTGACCGACAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGt    <  2:208124/139‑1 (MQ=255)
                                                                                                                                  aaaTACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTg   >  1:499882/1‑140 (MQ=255)
                                                                                                                                   aaTACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGa  >  2:358169/1‑140 (MQ=255)
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AACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAAGTGA  >  NC_000913/445424‑445694

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑