| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 27.0% | 61.3 / 16.3 | 37 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
| Reads supporting (aligned to +/- strand): ref base T (2/25); new base G (10/0); total (12/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.89e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646612‑646886 | ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGa < 2:50603/140‑1 (MQ=255) tGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGc‑cccc > 1:731686/1‑140 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGtt < 1:205076/127‑1 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCtt > 1:305066/1‑140 (MQ=255) cTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGc‑ccccct > 1:292169/1‑138 (MQ=255) aaaTAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGC‑CCCCctttt > 1:78573/1‑140 (MQ=255) aatTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCaa < 2:588866/140‑1 (MQ=255) tAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGC‑CCCCCTTTTGCaataa > 2:232415/1‑140 (MQ=255) aaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTTGAATGC‑CCCCCTTTTGTcataatt > 1:60254/1‑139 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGG‑CCCCCTTTTTtaataaatttttt > 2:345228/1‑133 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGC‑CCCCCTTTTTCTATAAAGTTTtcgc > 2:67014/1‑140 (MQ=255) gTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGC‑CCCCCTTTTTTTATAATTTTTGTGCCTCCCGCCGttg > 2:674164/1‑138 (MQ=255) ggATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCTAATGC‑CCCCCTTTTTCAATAATTTTTTCGCCCCCCGCTGGAGCCAg > 2:623125/1‑140 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCa < 2:687673/140‑1 (MQ=255) gTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGGATGC‑CCCCCTTTTTCAATAATTTTTTTGCCCCCCGCTGTAGGCCGCGCCgg > 2:311104/1‑140 (MQ=255) aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc < 1:437146/140‑1 (MQ=255) aGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc < 1:345228/140‑1 (MQ=255) cAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGC‑CCCCCTTTTTTAATAATGTTTTCGCCCCCCCCCCTAGCCCGCGCCGGcccccc > 2:96209/1‑140 (MQ=255) gccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgc < 1:201156/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:623125/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 2:305066/140‑1 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACcgcg < 1:674164/140‑1 (MQ=255) cTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGc < 2:682173/140‑1 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGGCGGATACGCCCCTTTTTG‑TATAATCTTTGCGCCTCCCGCCGGGGCCCCCGCAGGGCCCCCCCCCCCCcccgcgcatcga > 2:201156/1‑134 (MQ=255) cTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGa < 1:711740/140‑1 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGTGGGTCGCATTT‑CCCCTTTTTTTAATAATTTTTTTCCCTCCCCCCCTCGGCAGCGCAGGCCCCCCCCCCCCCCCCCCGCTCCAACCGTGGTATACCCGCTGATCggcc > 2:375159/1‑137 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGC‑CCCCCTTTTTCAATAATTTTTTCGCCCCGCGCCGTAGCCCGCGCCGGCCCCCCCCCCCCCCCCCCGCCGCAACGGTGGGATCGCCCTTGGTccccc > 2:701597/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGC‑CCCCCTTTTTCAATAAATTTTTCGCCCCCCCCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGCTGCAACCGTGGGATTGCCCTTGATCGccc > 2:512933/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGC‑CCCCCTTTTTTAATAATGTTTTTGCCTCGCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCGCGGTGCAACGTTGGGATTGCCGTTGGTCGccc > 2:190760/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGC‑CCCCCTTTTTTAATAAATTTTTTGCCCCCCGCCGTAGGCAGCGCCGGCCCCCCCCCCCCCCCCGCGCTGCAACGCTGGTATCGCCGTTGATCGccc > 2:776472/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGC‑CCCCCTTTTTCAATAATTTTTTTGCCCCCCGCCGTAGCCCGCGCCGGGCCCCCCCCCCCCCCCGCGGTGCAACGCTGGTATCGCCCCTGGTCGccc > 2:573190/1‑139 (MQ=255) gTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGGTCGAATGC‑CCCCCTTTTTCAATAAATTTTTCGCCCCGCGCCGTTGCCCGCGCCGGCCCCCCCCCCCCCCCCCCGGCGCCACCTTGGGATTGCCGTTGGTCGccc > 2:715408/1‑139 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 1:48149/140‑1 (MQ=255) aCTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGt < 2:339365/140‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTTGGATGC‑CCCCCTTTTTTAATAATTTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCCCGCTGCAACGTTGGTATCGCCGTTGATCGCCCTCAGGAg > 2:258985/1‑140 (MQ=255) tGCCGGAGATAATCGAGATCGGCGGGGGGTCGGATGC‑CCCCCTTTTTCAATAATGTTTTTGCCCCGCGCCGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCCGTGCAACCTTGGTGTCGCCGCTGGTCGCCCTccggaa > 2:166905/1‑135 (MQ=255) ccGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGa < 2:462218/140‑1 (MQ=255) ccGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGa < 1:776472/140‑1 (MQ=255) ccGGAGATAATCGAGATCGGCGGGGGGTTGGATGG‑CCCCCTTTTTCAATAATTTTTTCGCCTCGCGCTGGAGCCAGCGCAGGCCCCCCCCCCCCCCCCGCGCGGCAACGTTGGTATCGCCGTTGGTCGCCCTCaggaggg > 2:709123/1‑139 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:239654/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 2:265407/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 2:25036/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:701597/140‑1 (MQ=255) gagaTAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCa < 1:166905/140‑1 (MQ=255) gaTAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAg < 1:67014/140‑1 (MQ=255) aTCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTAt < 1:258985/140‑1 (MQ=255) gcggGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGc < 1:232415/140‑1 (MQ=255) gTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc < 1:573190/140‑1 (MQ=255) gTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTc < 1:715408/140‑1 (MQ=255) ttCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa < 2:60254/140‑1 (MQ=255) ttCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCa < 1:417767/140‑1 (MQ=255) | TTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGGGGTTCGAATGC‑CCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCA > NC_000913/646612‑646886 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |