Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,512,749	G→A	intergenic (‑43/‑293)	mntH ← / → nupC	Mn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,512,749	0	G	A	100.0%	135.0 / NA	39	intergenic (‑43/‑293)	mntH/nupC	Mn(2(+))/Fe(2(+)): H(+) symporter MntH/nucleoside:H(+) symporter NupC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/19);  total (20/19)

ATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAT  >  NC_000913/2512618‑2512872                                                                                                                                    |                                                                                                                            aTCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACga                                                                                                                     >  1:282059/1‑140 (MQ=255) aTCGCCGCAATGAACGCAGGTCCCATTAATGCG‑GCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACgag                                                                                                                    >  1:244659/1‑140 (MQ=255)  tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACgag                                                                                                                    >  1:100498/1‑140 (MQ=255)  tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACgag                                                                                                                    >  2:429093/1‑140 (MQ=255)  tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACgag                                                                                                                    >  2:131872/1‑140 (MQ=255)  tCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACgag                                                                                                                    >  1:83352/1‑140 (MQ=255)     ccgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAAt                                                                                                                 <  2:83352/140‑1 (MQ=255)      cgcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATg                                                                                                                >  1:592094/1‑140 (MQ=255)       gcAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGa                                                                                                               <  2:100498/140‑1 (MQ=255)                               gCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGAtt                                                                                       >  2:102184/1‑140 (MQ=255)                                  aGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTaa                                                                                              >  2:41089/1‑130 (MQ=255)                                  aGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTaa                                                                                              <  1:41089/130‑1 (MQ=255)                                   gCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtgg                                                                                   >  2:307102/1‑140 (MQ=255)                                    ccTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                  >  2:510220/1‑140 (MQ=255)                                    ccTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTgtggt                                                                                  >  1:113122/1‑140 (MQ=255)                                          cTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTtc                                                                            <  2:244659/140‑1 (MQ=255)                                             gcgcgcCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTctct                                                                         >  2:115393/1‑140 (MQ=255)                                               gcgcCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGa                                                                       <  1:412041/140‑1 (MQ=255)                                                   cgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGa                                                                   <  1:396064/140‑1 (MQ=255)                                                   cgccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGa                                                                   <  2:113122/140‑1 (MQ=255)                                                    gccCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTTTACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAc                                                                  <  1:465495/140‑1 (MQ=255)                                                     cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                 <  1:115393/140‑1 (MQ=255)                                                     cccGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGAcc                                                                 <  2:29475/140‑1 (MQ=255)                                                                ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                            <  1:360573/134‑1 (MQ=255)                                                                ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGAt                                                            >  2:360573/1‑134 (MQ=255)                                                                ctACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGAc                                                      >  1:555229/1‑140 (MQ=255)                                                                     ctcAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGa                                                 <  2:282059/140‑1 (MQ=255)                                                                          cgcgATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGa                                            >  2:594526/1‑140 (MQ=255)                                                                                    cGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACtt                                  <  2:52247/140‑1 (MQ=255)                                                                                            tgtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgtta                          <  1:139447/140‑1 (MQ=255)                                                                                             gtgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttat                         >  1:327386/1‑140 (MQ=255)                                                                                              tgCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATgttatg                        >  1:475108/1‑140 (MQ=255)                                                                                                     aaaaCATAGCCTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGt                 >  1:592812/1‑140 (MQ=255)                                                                                                               cTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAg       <  2:582892/140‑1 (MQ=255)                                                                                                               cTTTGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAg       <  1:102184/140‑1 (MQ=255)                                                                                                                 ttGCTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGca     <  2:592812/140‑1 (MQ=255)                                                                                                                    cTATGTTTCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAt  <  1:307102/140‑1 (MQ=255)                                                                                                                         tttCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt               >  1:562211/1‑122 (MQ=255)                                                                                                                         tttCATGCTATACCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGttt               <  2:562211/122‑1 (MQ=255)                                                                                                                                    |                                                                                                                            ATCGCCGCAATGAACGCAGGTCCCATTAATGCGAGCCTCATCTTGCGCGCCGCCCGTCCGCTGCTACTCTCAACGCGATAGTTCGTCATCTTGTGCCTCTAAAACATAGCCTTTGCTATGTTTCATGCTATGCCAAACGAGAATGATTATCAAATTCATTTAAATGGATTGTGGTGATTTCTCTGATAGACCAGGATTATGACTACGAAAAGATTGATGACTTGAATGTTATGGTTATGTTTAATGTTAGCACAT  >  NC_000913/2512618‑2512872

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑