Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT36.1% 41.0 / 27.2 36intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/23);  new base T (13/0);  total (13/23)
Fisher's exact test for biased strand distribution p-value = 4.33e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574
                                                                                                                                         |                                                                                                                                           
aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCa                                                                                                                                           >  1:933966/1‑140 (MQ=255)
aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                           <  1:949567/140‑1 (MQ=255)
  aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGa                                                                                                                                         >  2:443934/1‑140 (MQ=255)
       tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAt                                                                                                                                    <  2:173712/140‑1 (MQ=255)
         ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATAc                                                                                                                                  <  2:812449/140‑1 (MQ=255)
                 tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAGAAAAAAAATg                                                                                                                          >  2:751861/1‑140 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGa                                                                                                                        >  1:194034/1‑140 (MQ=255)
                    tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                       <  1:751861/140‑1 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACAAAAAAAAAAAATAGAGGTgg                                                                                                                   >  2:378138/1‑139 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATTGAGtttg                                                                                                                   >  1:1037831/1‑138 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAAATTGAGTTgg                                                                                                                   >  2:476930/1‑139 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGc                                                                                                                   <  1:1053057/140‑1 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGc                                                                                                                   <  1:691691/140‑1 (MQ=255)
                         cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAAAAAAAAAAGGAGGTgtc                                                                                                                  >  2:708527/1‑138 (MQ=255)
                             atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCGAATTTTTTTCAACAAATAAAAAAAACAGAGGTGCCctga                                                                                                              >  2:668536/1‑140 (MQ=255)
                              tatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAAGAGGTGGCatgat                                                                                                             >  2:358362/1‑140 (MQ=255)
                                    ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                       <  2:333733/140‑1 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATAGAGGTGTCATGATGAGCGaaaaa                                                                                                   >  2:125018/1‑138 (MQ=255)
                                         aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGta                                                                                                  <  1:358362/140‑1 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                <  1:747329/140‑1 (MQ=255)
                                           tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaat                                                                                                <  1:708527/140‑1 (MQ=255)
                                                 gTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCGCATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                          <  1:774564/140‑1 (MQ=255)
                                                  ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                                         <  2:620881/140‑1 (MQ=255)
                                                            aaCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGaa                                                                               <  1:476930/140‑1 (MQ=255)
                                                             aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAAt                                                                              <  2:286338/140‑1 (MQ=255)
                                                               tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                            <  1:183312/140‑1 (MQ=255)
                                                                gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCAACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAAAAATAAAAAAAAAGCAGGGAATaaa                                                                           >  2:290513/1‑139 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTTAAACAAAAAAAAAAAATGGAGGTGGCATGATGAGCGAAAAGAAAAAAAAAAAAGCAGGGAAAAAATTTACAgagaa                                                                >  2:789668/1‑139 (MQ=255)
                                                                               aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTc                                                            <  1:615445/140‑1 (MQ=255)
                                                                                   tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGACAAAAAAAAAAAATGGAGGTGGGCTGATGGGGGAAAGAAAAAAGAAAAAGGCAGTGAATAAGTTTGAAGAGAATGTCGCtc                                                        >  2:493027/1‑139 (MQ=255)
                                                                                   tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAAAAAAAAATGGAGGTGGCCTGATGGGCGGAAGGAATAAACAAAAAGGAGGGAAAAAAATGACAGAGATTGTTGCTa                                                        >  2:778738/1‑140 (MQ=255)
                                                                                      cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGGGAAAGTAATAAACAAAAGGCAGGGAATAAATTTACAGAGATTGTCGGTTACt                                                     >  2:26410/1‑140 (MQ=255)
                                                                                           aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                <  1:125018/140‑1 (MQ=255)
                                                                                            atatAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCg                                               <  1:123850/140‑1 (MQ=255)
                                                                                               taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTTGATTTTTTTAGACAAAAACAAAAAATAGGGTTGGTATGATTGGGGGGAGAAAATAAAAAAAAGAAGAGAATAAATTTAAAGAGAATGTTGCTGACTTTACCGccc                                            >  2:261067/1‑139 (MQ=255)
                                                                                               taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGATTTTTTCCAAAAAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCa                                            <  2:1073744/140‑1 (MQ=255)
                                                                                                      acaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTc                                     <  1:26410/140‑1 (MQ=255)
                                                                                                              ggTGGTAGTTTGTGGCGGGAATTTTTTCCAGACAAATACAAAATCTGGAGTTGCCTTGATGAGCGAAAGTAAAAAGAAACAGGAAGGGATAATGTTGACGGAGTTTGTCCCTAACTTTCCCGCCATGATTTCTACCCGaa                             <  2:415075/139‑1 (MQ=255)
                                                                                                               gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                            <  1:778738/140‑1 (MQ=255)
                                                                                                               gTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                            <  2:933966/140‑1 (MQ=255)
                                                                                                                    aGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGGTGAGGGAAAGTAAAAAAAAAAAAGCAGAGAAAAAGTTGACAGAGATTGTTGCTAACTTTACCGCCATGATTTTTTACCCAAAGCCtg                       >  2:741696/1‑140 (MQ=255)
                                                                                                                     gTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGGGGTGCCATGATGAGGGAAAATAATAAACAACAAGCAGGGAATAAGTTGAAAGAGATTGTTGCTAACTTTTTCGCCCTGATTTTTACCCCAAAGCCtga                      >  2:639777/1‑140 (MQ=255)
                                                                                                                      tttGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGGGGTGGCATGATGAGAGGAAAAAAAAAAAAAAAAGCAGTGAAAAAAGTGACAGAGATTGTGGCTAACTTTTCCCCCCCGATTTTTACCCCAAAACCtgat                     >  2:401328/1‑140 (MQ=255)
                                                                                                                       ttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                    <  1:639777/140‑1 (MQ=255)
                                                                                                                       ttGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCtgatg                    <  1:789668/140‑1 (MQ=255)
                                                                                                                        tgtgGCGGTGAATTTTTCCAGACAAAAACAAAAAATGGAGCTGCCATGGCGGGGGAAAGCAAAAAACAACAGGGAGGGGAAAAAGTGACAGAGGGTGTCGCCAACTTTACCTCCATAATTTCTACCCGAAAGCCcgatga                   >  2:850203/1‑140 (MQ=255)
                                                                                                                           ggCGGTTAATTTTTCAAGAGAAATACAAAAACTGGAGTTCCCATGATGAGAGAAAATAAAAAACAACAGGTAGTGATTAAGTTGACAGAGATTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                <  2:650125/140‑1 (MQ=255)
                                                                                                                             cGGTGAATTTTTTCCGACAAATACAAAAAATGGAGGTGCCATGATGAGGGAAAGTAATAAACAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTTCCCCCATGATTTCTACCCCCAAGCCTGATGACgtgg              >  2:286559/1‑140 (MQ=255)
                                                                                                                                         ccAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa  <  1:66118/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAA  >  NC_000913/3206298‑3206574

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑