| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,027,436 | 0 | T | G | 59.1% | 22.8 / 21.4 | 22 | intergenic (+208/+173) | fre/fadA | flavin reductase/3‑ketoacyl‑CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base T (3/6); new base G (6/7); total (9/13) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.74e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.59e-01 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGAC > NC_000913/4027301‑4027574 | aTGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGcgcg > 2:270506/1‑140 (MQ=255)aTGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCATGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGcgcg > 1:170624/1‑140 (MQ=255) tGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGt > 2:948416/1‑140 (MQ=255) tGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGt > 2:699278/1‑140 (MQ=255) tGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGt > 2:504466/1‑140 (MQ=255) tATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTAt < 1:504466/140‑1 (MQ=255) tATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTAt > 1:534561/1‑140 (MQ=255) tATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTAt < 2:904102/140‑1 (MQ=255) tATCTGGCAAACCATGCCCGATGCGACGCTGCCGCGTCTTATCGTGCCTACAAATAGACCGACACGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATAAGAGCTCCGATGCCTGATGCGACGCTTGCCGCGTCtt > 2:181549/1‑140 (MQ=14) aTCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTATc < 1:158117/140‑1 (MQ=255) aTCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTATc < 1:42749/140‑1 (MQ=255) tCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTATca > 2:34175/1‑139 (MQ=255) cTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTATcag < 2:400704/140‑3 (MQ=25) cTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTATcag < 2:534561/140‑3 (MQ=37) gCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGGCGCGTCTTATcaggcc < 1:798445/140‑6 (MQ=2) caaaccatgCCCGATGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt > 2:185387/7‑140 (MQ=2) gacaggCCCGATGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACa < 2:889282/135‑1 (MQ=255) gCCCGATGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGt < 1:185387/140‑1 (MQ=255) cccgATGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGTGCGTCTTATCAGGCCTACATTGGTg > 2:500006/4‑140 (MQ=255) tGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGt > 2:493169/1‑134 (MQ=255) tGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGt < 1:493169/134‑1 (MQ=255) aCGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCgg < 1:270506/140‑1 (MQ=255) c‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAg < 2:560429/140‑1 (MQ=255) gTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGAc < 2:310663/140‑1 (MQ=255) | ATGACGTATCTGGCAAACCATGCCCGATGCGACGCTGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGC‑ATGATGCCTGATGCGACGC‑TGTCGCGTCTTATCGTGCCTACAAATAGTCCGAACCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGCAATTGGTGCATGATGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACATTGGTGCCGGATCGGTAGAC > NC_000913/4027301‑4027574 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |