Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,216,799	A→C	N18H (AAT→CAT)	ymgC →	protein YmgC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,216,799	0	A	C	100.0%	239.0 / NA	60	N18H (AAT→CAT)	ymgC	protein YmgC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (31/29);  total (31/29)

CTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCA  >  NC_000913/1216677‑1216926                                                                                                                           |                                                                                                                                cTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGgtg                                                                                                                <  2:180699/140‑1 (MQ=255)     tttttGTCTGTCGCTTTAGACTGTACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCa                                                                                                            <  1:835793/140‑1 (MQ=255)     tttttGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCa                                                                                                            <  1:554092/140‑1 (MQ=255)     tttttGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCa                                                                                                            >  1:1087206/1‑140 (MQ=255)          gtctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACg                                                                                                       <  1:1036331/140‑1 (MQ=255)          gtctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACg                                                                                                       <  1:528634/140‑1 (MQ=255)          gtctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACg                                                                                                       <  2:515873/140‑1 (MQ=255)           tctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGc                                                                                                      >  2:432356/1‑140 (MQ=255)           tctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGc                                                                                                      <  2:114195/140‑1 (MQ=255)           tctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGc                                                                                                      >  1:464245/1‑140 (MQ=255)            ctgtcGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCa                                                                                                     >  1:899616/1‑140 (MQ=255)                cGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATggg                                                                                                 <  1:991141/140‑1 (MQ=255)                           ataCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTTACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAg                                                                                      <  2:1047209/140‑1 (MQ=255)                            taCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTa                                                                                               >  1:850424/1‑130 (MQ=255)                            taCACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTa                                                                                               <  2:850424/130‑1 (MQ=255)                              cacaGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCa                                                                                   <  2:11643/140‑1 (MQ=255)                              cacaGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCa                                                                                   <  2:1026212/140‑1 (MQ=255)                                 aGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCatat                                                                                >  1:114286/1‑140 (MQ=255)                                 aGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCatat                                                                                >  1:480011/1‑140 (MQ=255)                                   cATAATTTTATTGGGTTTATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTTAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCAtatta                                                                              >  2:141533/1‑140 (MQ=255)                                   cATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGt                                                                                                <  2:841362/122‑1 (MQ=255)                                   cATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGt                                                                                                >  1:841362/1‑122 (MQ=255)                                   cATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCAtatta                                                                              >  1:102051/1‑140 (MQ=255)                                    aTAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGt                                                                                     <  1:651805/132‑1 (MQ=255)                                    aTAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGt                                                                                     >  2:651805/1‑132 (MQ=255)                                      aaTTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTg                                                                           >  1:301856/1‑140 (MQ=255)                                      aaTTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTg                                                                           >  1:435162/1‑140 (MQ=255)                                      aaTTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTg                                                                           >  2:622534/1‑140 (MQ=255)                                        ttttATTGGGTTTATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTTAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGAt                                                                         <  1:141533/140‑1 (MQ=255)                                        ttttATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGAt                                                                         <  2:1016351/140‑1 (MQ=255)                                        ttttATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGAt                                                                         <  1:432356/140‑1 (MQ=255)                                        ttttATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGAt                                                                         <  2:464245/140‑1 (MQ=255)                                             ttGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCa                                                                                   <  1:1043868/125‑1 (MQ=255)                                             ttGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCa                                                                                   >  2:1043868/1‑125 (MQ=255)                                             ttGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGc                                                                    >  2:523418/1‑140 (MQ=255)                                             ttGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGc                                                                    >  2:777510/1‑140 (MQ=255)                                                  ttAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTAc                                                               >  2:947227/1‑140 (MQ=255)                                                           cTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGtt                                                      >  1:501999/1‑140 (MQ=255)                                                            tACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGAT‑‑GGCTTTACGCTTGCGTTTaa                                                   >  2:945886/1‑140 (MQ=255)                                                              cGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTaa                                                   <  2:1087206/140‑1 (MQ=255)                                                                aGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTaaaa                                                 >  1:517059/1‑140 (MQ=255)                                                                aGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTaaaa                                                 >  2:325516/1‑140 (MQ=255)                                                                aGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTaaaa                                                 <  2:555892/140‑1 (MQ=255)                                                                  gCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGa                                               >  1:725330/1‑140 (MQ=255)                                                                     gACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGa                                            >  1:267302/1‑140 (MQ=255)                                                                                ttCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGa                                 <  1:622534/140‑1 (MQ=255)                                                                                 tCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGAt                                <  2:517059/140‑1 (MQ=255)                                                                                     tCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGAttctt                            >  2:339429/1‑140 (MQ=255)                                                                                     tCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGAttctt                            >  2:186887/1‑140 (MQ=255)                                                                                     tCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGAttctt                            >  2:654092/1‑140 (MQ=255)                                                                                      cccAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGAttcttt                           <  2:725330/140‑1 (MQ=255)                                                                                          gagagaTTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGa                       >  2:735297/1‑140 (MQ=255)                                                                                          gagagaTTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGa                       >  1:962574/1‑140 (MQ=255)                                                                                          gagagaTTTATTTTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGa                       >  1:712392/1‑140 (MQ=255)                                                                                               atttatttTTCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATctct                  >  1:367171/1‑140 (MQ=255)                                                                                                     ttttCAATGTGCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGc            <  1:523418/140‑1 (MQ=255)                                                                                                             gtgCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTg    <  2:102051/140‑1 (MQ=255)                                                                                                             gtgCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTg    <  2:886338/140‑1 (MQ=255)                                                                                                               gCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCa  <  2:269131/140‑1 (MQ=255)                                                                                                               gCTTTGTTTAAACATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCa  <  2:899616/140‑1 (MQ=255)                                                                                                                           |                                                                                                                                CTTATTTTTGTCTGTCGCTTTAGACTATACACAGCATAATTTTATTGGGTTAATATTTCTACGAGGCTGACATGAATAATTCAATCCCAGAGAGATTTATTTTTCAATGTGCTTTGTTTAAAAATCTCGAGAGGGAGGTGTTCATGACGCATGGGTATGTTGATAGTCATATTATTGATCAGGCTTTACGCTTGCGTTTAAAAGATGAAACCAGTGTGATTCTTTCTGATCTCTATCTGCAAATATTGCA  >  NC_000913/1216677‑1216926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑