Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,679,675	Δ1 bp	coding (119/1383 nt)	ydgI →	putative arginine:ornithine antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,679,675	0	T	.	100.0%	84.3 / NA	21	coding (119/1383 nt)	ydgI	putative arginine:ornithine antiporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (8/13);  total (8/13)

AATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATG  >  NC_000913/1679542‑1679779                                                                                                                                      |                                                                                                         aaTCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGc                                                                                                   <  2:274111/140‑1 (MQ=255) aaTCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGACGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGc                                                                                                   <  2:292986/140‑1 (MQ=255)       tGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGc                                                                                                   <  2:386450/134‑1 (MQ=255)       tGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGc                                                                                                   >  1:386450/1‑134 (MQ=255)                               ggACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTg                                                                     <  1:286224/140‑1 (MQ=255)                                gACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGc                                                                    >  1:175385/1‑140 (MQ=255)                                     aGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTACCAGCCCGGCAGCACTGC‑CATCGGCTGGGGGATTACTGGCGCTGGCATTTTATTGCTGGcc                                                               >  2:138543/1‑140 (MQ=255)                                       cgcACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCCGGGGGGTTACTGGGGCCGGGATTTTATTGCTGGGCtt                                                             >  2:133886/1‑140 (MQ=255)                                           cTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGGATTACTGGCGCTGGCATTTTTTTTCTGGCCTTTGcc                                                         >  1:203179/1‑140 (MQ=255)                                                        tATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTACCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCa                                            <  1:138543/140‑1 (MQ=255)                                                          ttAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCAcg                                          <  2:212467/140‑1 (MQ=255)                                                              gCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCa                                      <  2:154540/140‑1 (MQ=255)                                                                         ggCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGGATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGaa                           >  1:401065/1‑140 (MQ=255)                                                                              gggTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGa                      <  2:128737/140‑1 (MQ=255)                                                                              gggTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGa                      >  1:344369/1‑140 (MQ=255)                                                                              gggTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGa                      <  1:189698/140‑1 (MQ=255)                                                                              gggTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGa                      >  2:311647/1‑140 (MQ=255)                                                                              gggTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGa                      >  1:128737/1‑140 (MQ=255)                                                                                  gtTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcggc                  <  1:311647/140‑1 (MQ=255)                                                                                   ttttCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGAcggcg                 <  2:348966/140‑1 (MQ=255)                                                                                     ttCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGt               <  2:175385/140‑1 (MQ=255)                                                                                                  aaaaTATGGCGGCAGTTGCCAGCCCGGCAGCACTGC‑CATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATg  <  2:389860/140‑1 (MQ=255)                                                                                                                                      |                                                                                                         AATCACTGGATATCGATGGAAAAGAAACTGGGACTGAGCGCACTCACCGCGCTGGTATTAAGCTCAATGCTGGGCGCGGGTGTTTTCAGTCTGCCGCAAAATATGGCGGCAGTTGCCAGCCCGGCAGCACTGCTCATCGGCTGGGGTATTACTGGCGCTGGCATTTTATTGCTGGCCTTTGCCATGCTGATCCTCACGCGCATTCGTCCCGAACTTGACGGCGGTATCTTTACCTATG  >  NC_000913/1679542‑1679779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑