Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,394,301	T→C	V79A (GTC→GCC)	nnr →	NAD(P)HX epimerase/NAD(P)HX dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,394,301	0	T	C	100.0%	95.2 / NA	27	V79A (GTC→GCC)	nnr	NAD(P)HX epimerase/NAD(P)HX dehydratase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (11/16);  total (11/16)

CTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCAT  >  NC_000913/4394165‑4394425                                                                                                                                         |                                                                                                                             cTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCcgc                                                                                                                           >  2:468747/1‑140 (MQ=255)       cTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACt                                                                                                                     >  1:591103/1‑140 (MQ=255)              tctATGAGCTGTTGCTTCGCGCTGGCGGGGCCGCTTCCCGGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCaaa                                                                                                              <  2:322934/140‑1 (MQ=255)                tATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGc                                                                                                            >  2:36046/1‑140 (MQ=255)                   gAGCTGATGCTTCGCGCTGGCGGGGCCGCATTCCGGGTGTGTCGTTGGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGt                                                                                                         <  2:494123/140‑1 (MQ=255)                    aGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTc                                                                                                        >  1:158177/1‑140 (MQ=255)                     gCTGATGCTTCGCGCTGGGGGGGCCGCATTCCAGGTGTGTCGTCCGGCGTATCCTGCCGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCg                                                                                                       <  2:568514/140‑1 (MQ=255)                        gATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCa                                                                                                    >  2:488517/1‑140 (MQ=255)                        gATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCa                                                                                                    >  1:494123/1‑140 (MQ=255)                          tGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCAtt                                                                                                  >  1:62670/1‑140 (MQ=255)                           gCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTCCGGCGTATCCTGCCGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTg                                                                                                 <  2:75304/140‑1 (MQ=255)                                      gCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACgttg                                                                                      <  1:488517/140‑1 (MQ=255)                                       cGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACgttgt                                                                                     <  2:591103/140‑1 (MQ=255)                                         aGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGttgttg                                                                                   <  2:62670/140‑1 (MQ=255)                                                      ggTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGGCACGTTGTTTGGCCAGGAGGGCg                                                                      >  2:454992/1‑140 (MQ=255)                                                              gTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCg                                                              <  1:468747/140‑1 (MQ=255)                                                               ttCGGCGTATCCTGCCGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGt                                                             <  1:349047/140‑1 (MQ=255)                                                                             aCGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGGCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGcc                                               >  2:503015/1‑140 (MQ=255)                                                                                cccgccACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCcgcg                                            <  2:480542/140‑1 (MQ=255)                                                                                 ccgccACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCgcgc                                           >  2:348533/1‑140 (MQ=255)                                                                                   gccACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTg                                         >  1:59737/1‑140 (MQ=255)                                                                                        tggctggTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCAcg                                    <  1:348533/140‑1 (MQ=255)                                                                                                    tgCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCAcgag                                  >  2:336802/1‑128 (MQ=255)                                                                                                    tgCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCAcgag                                  <  1:336802/130‑3 (MQ=255)                                                                                                         tCATGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAAcg                   >  2:199825/1‑140 (MQ=255)                                                                                                            tGGTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGAATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCgg                <  1:378060/140‑1 (MQ=255)                                                                                                              gTAATAACGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGt              >  1:249478/1‑140 (MQ=255)                                                                                                                 ataaCGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGc           <  1:36046/140‑1 (MQ=255)                                                                                                                   aaCGGCGGCGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCga         >  2:347890/1‑140 (MQ=255)                                                                                                                      ggcggcGATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGAt      <  2:249478/140‑1 (MQ=255)                                                                                                                        cggcgATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATcc    >  1:402864/1‑140 (MQ=255)                                                                                                                          gcgATGGCTACGTGGCCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCAt  <  1:454992/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             CTGGGGCTCACACTCTATGAGCTGATGCTTCGCGCTGGCGAGGCCGCATTCCAGGTGTGTCGTTCGGCGTATCCTGACGCCCGCCACTGGCTGGTGCTGTGCGGTCATGGTAATAACGGCGGCGATGGCTACGTGGTCGCGCGACTGGCCAAAGCGGTCGGCATTGAGGTCACGTTGTTGGCCCAGGAGAGCGACAAACCGTTGCCGGAAGAGGCCGCGCTGGCACGCGAAGCATGGTTAAACGCGGGTGGCGAGATCCAT  >  NC_000913/4394165‑4394425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑