Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,979,502	A→G	intergenic (‑287/‑251)	insA‑5 ← / → uspC	IS1 protein InsA/universal stress protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,979,502	0	A	G	100.0%	165.4 / NA	42	intergenic (‑287/‑251)	insA‑5/uspC	IS1 protein InsA/universal stress protein C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (22/20);  total (22/20)

AATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGAT  >  NC_000913/1979369‑1979630                                                                                                                                      |                                                                                                                                 aaTGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGca                                                                                                                            >  2:271290/1‑140 (MQ=255) aaTGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGca                                                                                                                            >  1:381579/1‑140 (MQ=255)             cgcACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACtta                                                                                                                >  2:77480/1‑140 (MQ=255)               cacaGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACttaat                                                                                                              >  1:17348/1‑140 (MQ=255)                    tCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACttaatgttaa                                                                                                         >  2:540261/1‑140 (MQ=255)                      aaaaTAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACttaatgttaatg                                                                                                       >  1:408468/1‑140 (MQ=255)                      aaaaTAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACttaatgttaatg                                                                                                       <  1:271290/140‑1 (MQ=255)                        aaTAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGaa                                                                                                     <  1:408430/140‑1 (MQ=255)                        aaTAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGaa                                                                                                     <  2:17348/140‑1 (MQ=255)                            aCTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGt                                                                                                 <  2:381579/140‑1 (MQ=255)                            aCTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGt                                                                                                 >  1:174156/1‑140 (MQ=255)                             cTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGtt                                                                                                <  1:322622/140‑1 (MQ=255)                              tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGttt                                                                                               >  2:592113/1‑140 (MQ=255)                              tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGttt                                                                                               >  2:592835/1‑140 (MQ=255)                              tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGttt                                                                                               >  2:304087/1‑140 (MQ=255)                              tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGttt                                                                                               >  2:207470/1‑140 (MQ=255)                              tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGtt                                                                                                <  2:474843/139‑1 (MQ=255)                              tCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGtt                                                                                                >  1:474843/1‑139 (MQ=255)                                aCCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTa                                                                                             <  2:452214/140‑1 (MQ=255)                                aCCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTa                                                                                             >  1:452214/1‑140 (MQ=255)                                aCCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTa                                                                                             <  1:540261/140‑1 (MQ=255)                                 ccGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTAc                                                                                            <  2:408468/140‑1 (MQ=255)                                 ccGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTAc                                                                                            <  1:224299/140‑1 (MQ=255)                                 ccGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTAc                                                                                            <  2:71442/140‑1 (MQ=255)                                 ccGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTAc                                                                                            <  1:468748/140‑1 (MQ=255)                                        taatCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATAtt                                                                                     <  1:207470/140‑1 (MQ=255)                                                                tttCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAAt                                                             <  1:592835/140‑1 (MQ=255)                                                                tttCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAAt                                                             <  1:592113/140‑1 (MQ=255)                                                                  tCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCa                                                           >  1:392301/1‑140 (MQ=255)                                                                          tACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATTTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTa                                                   >  1:323555/1‑140 (MQ=255)                                                                          tACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTa                                                   >  2:257298/1‑140 (MQ=255)                                                                          tACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTa                                                   >  1:327331/1‑140 (MQ=255)                                                                            ccAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTAtt                                                 <  2:344966/140‑1 (MQ=255)                                                                                      tATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAAt                                       <  2:482421/140‑1 (MQ=255)                                                                                            tAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACAtt                                 <  2:392301/140‑1 (MQ=255)                                                                                                         aCCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGa                    >  1:531308/1‑140 (MQ=255)                                                                                                         aCCGCACAACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGa                    >  1:157443/1‑140 (MQ=255)                                                                                                             cacaACAAACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCAc                >  1:219042/1‑140 (MQ=255)                                                                                                               caacaaACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCg              >  1:313659/1‑140 (MQ=255)                                                                                                                 acaaACTAATTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCggg            >  1:22925/1‑140 (MQ=255)                                                                                                                        aaTTAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGtgatt     <  2:184818/140‑1 (MQ=255)                                                                                                                           tAAATACAAAGGAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGtgattgat  <  2:313659/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                 AATGGGATGTTGCGCACAGTCAAAATAACTCACCGTAAATAATCATCTGCTATAAATAATCACTTTCATGCAATACCAGATAAGCTATTTTTAAACAGACACTTACCGCACAACAAACTAATTAAATACAAAGAAAGGCAACAATCAACTTAATGTTAATGAAATGTTTTACATATATTAACCACTTGTTTAATGCGGAGAATCAGTACACTATTTTCTTATAATTACATTTGAAATTATATGATCACCGGGAGTGATTGAT  >  NC_000913/1979369‑1979630

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑