Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	753,125	0	T	A	50.0%	15.4 / 10.3	20	intergenic (‑330/+60)	ybgD/gltA	putative fimbrial protein YbgD/citrate synthase
Reads supporting (aligned to +/- strand):  ref base T (10/0);  new base A (0/10);  total (10/10)
Fisher's exact test for biased strand distribution p-value = 1.08e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCG  >  NC_000913/752999‑753263                                                                                                                               |                                                                                                                                           gACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCg                                                                                                                               >  2:388647/1‑140 (MQ=255)             ttttttAATTGAAAGTTTTGGGTTGCTGATATTTTGAGTTTTTTTATTTTTTTTAAATATTTATATAGGTTTGTTAATGGTTTTTTTTTTTACATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAAcgg                                                                                                                   <  2:468195/139‑1 (MQ=255)                    aTTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTTTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTa                                                                                                            <  1:133752/140‑1 (MQ=255)                      tGAAAGTTTTGGTTTGCTGATAATTTGAGTTTTTTTATTTTTTTTAAATATTTATATAGGTTTGTTAATGGATTTTATTTTTAAAATTTTTTGTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTaaa                                                                                                          <  2:267055/140‑1 (MQ=255)                      tGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTTTTTTTAAATATCTATATAGGTCTGTTAATGAATTTTTTTTTTACATTTTTTTGTGTTTAGGCATAAAAAAACCACCCCGCCATATGAACGGCGGGTTaaa                                                                                                          <  1:316160/140‑1 (MQ=255)                                         atatTTTGAGTTTTTTTTTTTTTTTAAAATATGTATATAGGTTTGTTAATGTATTTTATTTTTAAAATTTTTTGTTTTTAGATATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAAt                                                                                       <  2:20540/140‑1 (MQ=255)                                                             ttttttAAATATCTATATAGGTTTGTTAATGGTTTTTTTTTTTAAATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGatat                                                                   <  2:360101/139‑1 (MQ=255)                                                                tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                >  1:360101/1‑140 (MQ=255)                                                                tttAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGc                                                                >  2:281939/1‑140 (MQ=255)                                                                                 gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                               >  1:13438/1‑140 (MQ=255)                                                                                 gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                               >  2:418697/1‑140 (MQ=255)                                                                                 gTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGGTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGtttt                                               >  2:95012/1‑140 (MQ=255)                                                                                    tGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCa                                            >  2:46949/1‑140 (MQ=255)                                                                                     tttAATGTATTTTATTTTTACAATTTTTTGTTTTTAGGCATAAAAAAACAAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                           <  1:245634/139‑1 (MQ=255)                                                                                     tttAATGGTTTTTATTTTTACATTTTTTTGTGTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCat                                           <  1:388647/139‑1 (MQ=255)                                                                                      ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                          >  1:445705/1‑140 (MQ=255)                                                                                      ttAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCata                                          >  2:341816/1‑140 (MQ=255)                                                                                        aaTGGTTTTTATTTTTAAATTTTTTTTTTTTTAGGCATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATc                                        <  2:313149/140‑1 (MQ=255)                                                                                           ggATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTg                                     >  1:279763/1‑140 (MQ=255)                                                                                                        aaatttttttGTTTTTAGGAATAAAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGAcg                        <  2:13438/136‑1 (MQ=255)                                                                                                                             tataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATAcc   >  2:83638/1‑140 (MQ=255)                                                                                                                              ataAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCg  <  1:341816/140‑1 (MQ=255)                                                                                                                               |                                                                                                                                           GACTACCATGAAGTTTTTAATTGAAAGTATTGGGTTGCTGATAATTTGAGCTGTTCTATTCTTTTTAAATATCTATATAGGTCTGTTAATGGATTTTATTTTTACAATTTTTTGTGTTTAGGCATATAAAAATCAACCCGCCATATGAACGGCGGGTTAAAATATTTACAACTTAGCAATCAACCATTAACGCTTGATATCGCTTTTAAAGTCGCGTTTTTCATATCCTGTATACAGCTGACGCGGACGGGCAATCTTCATACCG  >  NC_000913/752999‑753263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑