Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,677,696	G→A	L219L (CTG→TTG)	yhjG ←	AsmA family protein YhjG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,677,696	0	G	A	100.0%	59.5 / NA	18	L219L (CTG→TTG)	yhjG	AsmA family protein YhjG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

TTACCAGAGCGGAAATCAGCCTGTACCGGAAACGGCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATC  >  NC_000913/3677575‑3677825                                                                                                                          |                                                                                                                                  ttACCAGAGCGGAAATCAGCCTGTACCGGAAACGGCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGcc                                                                                                                 <  2:138348/140‑1 (MQ=255) ttACCAGAGCGGAAATCAGCCTGTACCGGAAACGGCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGcc                                                                                                                 <  2:135419/140‑1 (MQ=255)     cAGAGCGGAAATCAGCCTGTACCGGAAACGGCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCAcc                                                                                                             <  1:419337/140‑1 (MQ=255)                                  ggCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATcc                                                                                >  1:219697/1‑140 (MQ=255)                                   gCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCa                                                                               <  1:488626/140‑1 (MQ=255)                                    cGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAg                                                                              >  1:360906/1‑140 (MQ=255)                                                    aGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACgg                                                              >  1:406012/1‑140 (MQ=255)                                                      cgcCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCa                                                            <  2:266298/140‑1 (MQ=255)                                                           gCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGc                                                       >  1:317630/1‑140 (MQ=255)                                                            cATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCt                                                      <  2:406012/140‑1 (MQ=255)                                                                      aTTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAggg                                            >  1:392079/1‑140 (MQ=255)                                                                                 aCCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACaaaaa                                 >  1:114066/1‑140 (MQ=255)                                                                                 aCCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACaaaaa                                 >  2:145749/1‑140 (MQ=255)                                                                                             ttCACCGTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCg                     >  1:391135/1‑140 (MQ=255)                                                                                                   gTTATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGc               <  2:219697/140‑1 (MQ=255)                                                                                                    ttATATCGTCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCt              <  2:392079/140‑1 (MQ=255)                                                                                                            tCCCTGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGt      >  2:46200/1‑140 (MQ=255)                                                                                                                tGCGCCTTCAAGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGtcatc  <  2:317630/140‑1 (MQ=255)                                                                                                                          |                                                                                                                                  TTACCAGAGCGGAAATCAGCCTGTACCGGAAACGGCGTCCCTTCGCCACGCAGCGCCAGCATACCGCCTATTTTTCCCGTACCCGTGAGCGGTTCACCGTTATATCGTCCCTGCGCCTTCAGGCCAAAAACGTAATCGCCCACCTTTTCTTTATCCGCTTTACCTTTCGATCCAGTAACTTCGCTGAACGGCAGCGGCTTGCCTAAGGGATCAACAAAAATCTCCAGATCCGCTTTGCTTACTTTGTCATC  >  NC_000913/3677575‑3677825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑