Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,306,101	C→A	S113* (TCG→TAG)	oppF →	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,306,101	0	C	A	100.0%	39.9 / NA	13	S113* (TCG→TAG)	oppF	murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (8/5);  total (8/5)

GCCTGGTTAGGTAAAGAGTTGCTGGGCATGAAACCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATCGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGT  >  NC_000913/1306007‑1306220                                                                                               |                                                                                                                        gCCTGGTTAGGTAAAGAGTTGCTGGGCATGAAACCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACt                                                                            <  1:257428/140‑1 (MQ=255)                     gCTGGGCATGAAACCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGaaaa                                                        >  1:46078/1‑140 (MQ=255)                           cATGAAACCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCAc                                                  >  2:403763/1‑140 (MQ=255)                               aaaCCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCa                                              >  2:96660/1‑140 (MQ=255)                                aaCCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAg                                             >  2:102334/1‑140 (MQ=255)                                                     ttCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAg                        >  2:483316/1‑140 (MQ=255)                                                         cAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCga                    <  1:96660/140‑1 (MQ=255)                                                           gTGATATTCAGATGATTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCgatg                  <  2:187830/140‑1 (MQ=255)                                                                      atgatTTTCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGt       <  1:403763/140‑1 (MQ=255)                                                                           tttCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCga                    <  1:816/122‑1 (MQ=255)                                                                           tttCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCga                    >  2:816/1‑122 (MQ=255)                                                                           tttCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGt  >  2:239746/1‑140 (MQ=255)                                                                           tttCCAGGATCCGTTGGCATAGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGt  >  2:339472/1‑140 (MQ=255)                                                                                               |                                                                                                                        GCCTGGTTAGGTAAAGAGTTGCTGGGCATGAAACCCGATGAATGGCGTGCCGTTCGCAGTGATATTCAGATGATTTTCCAGGATCCGTTGGCATCGCTAAACCCGCGTATGACCATCGGCGAGATCATCGCTGAACCACTGCGTACTTATCATCCGAAAATGTCACGCCAGGAAGTTCGCGAGCGCGTGAAGGCGATGATGCTGAAAGTCGGGT  >  NC_000913/1306007‑1306220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑