Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,618,033	G→C	A336P (GCG→CCG)	ydeA →	L‑arabinose exporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,618,033	0	G	C	100.0%	152.5 / NA	39	A336P (GCG→CCG)	ydeA	L‑arabinose exporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (16/23);  total (16/23)

ACCTGCGGCGAACAGTGAAATACACCTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGGCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTC  >  NC_000913/1617903‑1618166                                                                                                                                   |                                                                                                                                      aCCTGCGGCGAACAGTGAAATACACCTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATtct                                                                                                                              >  2:11530/1‑140 (MQ=255)           aaCAGTGAAATACACCTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATAtt                                                                                                                    >  1:60037/1‑140 (MQ=255)                 gAAATACACCTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCAGCATATTTAatat                                                                                                              <  1:339519/140‑1 (MQ=255)                             ggggTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGc                                                                                                  >  2:413797/1‑140 (MQ=255)                                    tGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCg                                                                                           <  1:481329/140‑1 (MQ=255)                                    tGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCg                                                                                           >  2:277947/1‑140 (MQ=255)                                      aGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGtt                                                                                         >  1:156512/1‑140 (MQ=255)                                           tttCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTggt                                                                                      <  2:230347/138‑1 (MQ=255)                                           tttCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTggt                                                                                      >  1:230347/1‑138 (MQ=255)                                             tCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAGGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTggtaggt                                                                                  <  2:156512/140‑1 (MQ=255)                                             tCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTggtaggt                                                                                  >  1:36284/1‑140 (MQ=255)                                             tCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTggtaggt                                                                                  >  1:200280/1‑140 (MQ=255)                                                  gggATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCa                                                                             >  2:375082/1‑140 (MQ=255)                                                  ggaaTCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCa                                                                             >  2:160759/4‑140 (MQ=255)                                                    gATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAgg                                                                           <  1:375082/140‑1 (MQ=255)                                                    gATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAgg                                                                           <  2:123842/140‑1 (MQ=255)                                                    gATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAgg                                                                           <  2:200280/140‑1 (MQ=255)                                                    gATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAgg                                                                           <  2:319687/140‑1 (MQ=255)                                                         cgATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGt                                                                      <  1:277947/140‑1 (MQ=255)                                                              atgatCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCa                                                                 <  2:234570/140‑1 (MQ=255)                                                                     tcGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCa                                                          <  1:408452/140‑1 (MQ=255)                                                                     tcGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATAATTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCa                                                          <  1:223090/140‑1 (MQ=255)                                                                      cGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCa                                                                 >  1:4806/1‑132 (MQ=255)                                                                      cGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCa                                                                 <  2:4806/132‑1 (MQ=255)                                                                        ggCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCa                                                                 <  1:86585/130‑1 (MQ=255)                                                                        ggCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCa                                                                 >  2:86585/1‑130 (MQ=255)                                                                        ggCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATg                                                       >  1:198963/1‑140 (MQ=255)                                                                         gCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGTGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCa                                                          <  2:129741/136‑1 (MQ=255)                                                                         gCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGTGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCa                                                          >  1:129741/1‑136 (MQ=255)                                                                         gCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGt                                                      <  2:21908/140‑1 (MQ=255)                                                                          cTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTc                                                     <  2:431820/140‑1 (MQ=255)                                                                           ttGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGt                                                                      <  1:346221/122‑1 (MQ=255)                                                                           ttGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGt                                                                      >  2:346221/1‑122 (MQ=255)                                                                           ttGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCg                                                    <  1:11530/140‑1 (MQ=255)                                                                           ttGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCg                                                    <  1:154822/140‑1 (MQ=255)                                                                                                                  cTACCGACGTCGCGATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCcgcg             <  2:198963/140‑1 (MQ=255)                                                                                                                            cgcgATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGt   <  2:36284/140‑1 (MQ=255)                                                                                                                             gcgATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTc  <  1:413797/140‑1 (MQ=255)                                                                                                                             gcgATGCCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTc  >  1:334441/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                      ACCTGCGGCGAACAGTGAAATACACCTCGGGGTGCTGAGTATTTTCTGGGGGATCGCGATGATGATCATCGGGCTTGGTATGCAGGTTAAAGTGCTGGCGCTGGCACCAGATGCTACCGACGTCGCGATGGCGCTATTCTCCGGCATATTTAATATTGGAATCGGGGCGGGTGCGTTGGTAGGTAATCAGGTGAGTTTGCACTGGTCAATGTCGATGATTGGTTATGTGGGCGCGGTGCCTGCTTTTGCCGCGTTAATTTGGTC  >  NC_000913/1617903‑1618166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑