Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,619,296	Δ1 bp	coding (177/435 nt)	marR →	DNA‑binding transcriptional repressor MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,619,295	0	A	.	100.0%	174.4 / NA	41	coding (176/435 nt)	marR	DNA‑binding transcriptional repressor MarR
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (20/21);  total (20/21)

TTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCG  >  NC_000913/1619159‑1619429                                                                                                                                         |                                                                                                                                       ttGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGactg                                                                                                                                     >  1:543505/1‑137 (MQ=255)    ggTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGaaa                                                                                                                                 >  1:77092/1‑140 (MQ=255)                  tatGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTgtcggt                                                                                                                   <  2:543505/140‑1 (MQ=255)                     ggTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGa                                                                                                                <  2:67272/140‑1 (MQ=255)                         aaTCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTg                                                                                                            >  1:146168/1‑140 (MQ=255)                          aTCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTgg                                                                                                           >  1:491689/1‑140 (MQ=255)                          aTCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGCTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTgg                                                                                                           >  2:349048/1‑140 (MQ=255)                               aagaaagaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCa                                                                                                      >  1:16700/1‑140 (MQ=255)                                    agaTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGTGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGAc                                                                                                 <  1:747748/140‑1 (MQ=255)                                      aTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGAccc                                                                                               <  2:847605/140‑1 (MQ=255)                                      aTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGAccc                                                                                               <  2:146168/140‑1 (MQ=255)                                      aTCGCCTGCTTAACGAGTATCTGTCTCCGCTGGAGATTACCGCGGCACAGTTTAAGGTGCTCTGCTGTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑GCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGAcgc                                                                                               <  2:551320/140‑3 (MQ=255)                                         gCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCATTGACCCGTa                                                                                            >  2:656618/1‑140 (MQ=255)                                         gCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTa                                                                                            >  2:975959/1‑140 (MQ=255)                                         gCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTa                                                                                            >  2:258960/1‑140 (MQ=255)                                                    gAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGc                                                                                 <  1:349048/140‑1 (MQ=255)                                                    gAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGc                                                                                 <  2:16700/140‑1 (MQ=255)                                                       tATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTg                                                                              <  2:241380/140‑1 (MQ=255)                                                        aTCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTgg                                                                             <  1:258960/140‑1 (MQ=255)                                                        aTCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTgg                                                                             <  2:642340/140‑1 (MQ=255)                                                        aTCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTgg                                                                             <  2:16055/140‑1 (MQ=255)                                                         tCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGt                                                                            >  2:422844/1‑140 (MQ=255)                                                                  gCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAgg                                                                   <  1:422844/140‑1 (MQ=255)                                                                  gCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAgg                                                                   <  1:419168/140‑1 (MQ=255)                                                                           tACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGa                                                          >  2:884402/1‑140 (MQ=255)                                                                                cgGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGt                                                     >  1:237032/1‑140 (MQ=255)                                                                                 ggCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGtt                                                    >  2:245055/1‑140 (MQ=255)                                                                                  gCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCt                                                                 >  2:192741/1‑126 (MQ=255)                                                                                  gCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCt                                                                 <  1:192741/126‑1 (MQ=255)                                                                                    acaGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGcc                                                 <  1:884402/140‑1 (MQ=255)                                                                                        tttAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAAc                                             >  2:136486/1‑140 (MQ=255)                                                                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACa                                   >  2:915451/1‑140 (MQ=255)                                                                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACa                                   >  2:932031/1‑140 (MQ=255)                                                                                                  tctgctctATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACa                                   >  1:111591/1‑140 (MQ=255)                                                                                                      ctctATCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcg                               >  2:242823/1‑140 (MQ=255)                                                                                                           tCCGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCgcggcg                          >  2:693890/1‑140 (MQ=255)                                                                                                            ccGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGt                         <  1:915451/140‑1 (MQ=255)                                                                                                            ccGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGt                         <  2:237032/140‑1 (MQ=255)                                                                                                            ccGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGt                         <  1:693890/140‑1 (MQ=255)                                                                                                            ccGCTGCGCGGCGTGTATTACTCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGt                         <  1:975959/140‑1 (MQ=255)                                                                                                                                 tCCGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcgg    <  1:396160/140‑1 (MQ=255)                                                                                                                                   cGGTTG‑ACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACcggcg  >  2:318385/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       TTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAAGATCGCCTGCTTAACGAGTATCTGTCTCCGCTGGATATTACCGCGGCACAGTTTAAGGTGCTCTGCTCTATCCGCTGCGCGGCGTGTATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCG  >  NC_000913/1619159‑1619429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑