Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,509	T→C	intergenic (‑352/+91)	rrsG ← / ← clpB	16S ribosomal RNA/ClpB chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,509	0	T	C	100.0%	171.1 / NA	43	intergenic (‑352/+91)	rrsG/clpB	16S ribosomal RNA/ClpB chaperone
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/22);  total (21/22)

GCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAA  >  NC_000913/2731377‑2731643                                                                                                                                     |                                                                                                                                       gCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCa                                                                                                                                 >  2:377773/1‑140 (MQ=255)      tctcTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACtttt                                                                                                                            <  2:749728/140‑1 (MQ=255)              gagaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaaa                                                                                                                    <  2:687912/140‑1 (MQ=255)              gagaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaaa                                                                                                                    <  2:54210/140‑1 (MQ=255)                gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAAc                                                                                                                  <  1:377773/140‑1 (MQ=255)                gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAAc                                                                                                                  <  2:401746/140‑1 (MQ=255)                gaTTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAAc                                                                                                                  <  2:572261/140‑1 (MQ=255)                    ctcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaa                                                                                                                     >  2:391544/1‑133 (MQ=255)                    ctcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaa                                                                                                                     <  1:391544/133‑1 (MQ=255)                    ctcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCt                                                                                                              >  1:368739/1‑140 (MQ=255)                    ctcAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCt                                                                                                              <  2:666416/140‑1 (MQ=255)                      cAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaaa                                                                                                                    <  2:37344/132‑1 (MQ=255)                      cAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaaa                                                                                                                    >  1:37344/1‑132 (MQ=255)                         gagaACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAat                                                                                                         <  1:791230/140‑1 (MQ=255)                              ccccGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaa                                                                                                                     <  2:157619/123‑1 (MQ=255)                              ccccGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCaa                                                                                                                     >  1:157619/1‑123 (MQ=255)                              ccccGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGt                                                                                                    >  2:471302/1‑140 (MQ=255)                              ccccGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGt                                                                                                    >  2:74552/1‑140 (MQ=255)                                 cgccgACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCtt                                                                                                 >  1:359105/1‑140 (MQ=255)                                       cccGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGt                                                                                           <  2:201445/140‑1 (MQ=255)                                                gtgtTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTtat                                                                                  <  2:359105/140‑1 (MQ=255)                                                                cGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATGGACAAAAACGAGccc                                                                  >  2:656114/1‑140 (MQ=255)                                                                   gtCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGa                                                               <  1:471302/140‑1 (MQ=255)                                                                        tggtggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGc                                                          <  2:21039/140‑1 (MQ=255)                                                                        tggtggCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGc                                                          <  1:717095/140‑1 (MQ=255)                                                                         ggtggCGCATTATAGGGAGTTATTCCGGCCTGACGAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCt                                                         <  2:149061/140‑1 (MQ=255)                                                                             gcgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtt                                                     >  2:857241/1‑140 (MQ=255)                                                                             gcgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGtt                                                     <  1:409112/140‑1 (MQ=255)                                                                              cgcATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGttt                                                    >  1:459501/1‑140 (MQ=255)                                                                                  ttATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATc                                                <  2:459501/140‑1 (MQ=255)                                                                                  ttATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATc                                                >  1:654904/1‑140 (MQ=255)                                                                                            ttATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGa                                      >  2:542216/1‑140 (MQ=255)                                                                                             tATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGAc                                     >  2:46572/1‑140 (MQ=255)                                                                                             tATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGAc                                     >  2:410995/1‑140 (MQ=255)                                                                                             tATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGAc                                     >  2:175704/1‑140 (MQ=255)                                                                                               ttCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACgg                                   >  1:451403/1‑140 (MQ=255)                                                                                                  cGGCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGa                                <  1:919605/140‑1 (MQ=255)                                                                                                    gCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACa                              >  2:592588/1‑140 (MQ=255)                                                                                                    gCCTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACa                              >  1:589972/1‑140 (MQ=255)                                                                                                      cTGACAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAAt                            <  1:46572/140‑1 (MQ=255)                                                                                                         aCAAGCGAAAAATATAAAAACTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCg                         >  2:837098/1‑140 (MQ=255)                                                                                                                           aaCTTTATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAg       <  1:645278/140‑1 (MQ=255)                                                                                                                                tATCGCTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGaa  >  2:829567/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                       GCTTTTCTCTGTTGAGATTCTCAGGAGAACCCCGCCGACCCGGCGGCGTGTTTGCCGTTGTTCCGTGTCAGTGGTGGCGCATTATAGGGAGTTATTCCGGCCTGACAAGCGAAAAATATAAAAACTTTATCGTTCGCTCACTTTTCAGGCAAAACATCTTAAATATAGTCTTTTCCGTCTAACTTATAGACAAAAACGAGCCCCGAAGGGCTCGTTTTATCATTTACTGGACGGCGACAATCCGGTCTTCATTAACTTCCAGGCGAA  >  NC_000913/2731377‑2731643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑