Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,184,973	A→G	D45G (GAC→GGC)	ubiK →	ubiquinone biosynthesis accessory factor UbiK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,184,973	0	A	G	100.0%	285.7 / NA	72	D45G (GAC→GGC)	ubiK	ubiquinone biosynthesis accessory factor UbiK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/40);  total (32/40)

ATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAAGCAGCCAGATCCAGAG  >  NC_000913/3184840‑3185109                                                                                                                                      |                                                                                                                                         aTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    <  2:335659/140‑1 (MQ=255) aTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    <  2:27702/140‑1 (MQ=255) aTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    <  2:788726/140‑1 (MQ=255) aTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    <  2:76759/140‑1 (MQ=255) aTGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    >  1:636629/1‑140 (MQ=255)      tGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGcc                                                                                                                               <  1:569307/140‑1 (MQ=255)       ggcccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCg                                                                                                                              >  1:490291/3‑140 (MQ=255)       gACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    >  2:497615/1‑134 (MQ=255)       gACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGt                                                                                                                                    <  1:497615/134‑1 (MQ=255)         cccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTg                                                                                                                            >  2:936978/1‑140 (MQ=255)         cccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTg                                                                                                                            >  2:275984/1‑140 (MQ=255)         cccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTg                                                                                                                            <  2:118918/140‑1 (MQ=255)         cccGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTg                                                                                                                            >  1:434232/1‑140 (MQ=255)             aaaaaaaTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCATACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAaga                                                                                                                        >  2:323771/1‑140 (MQ=255)              aaaaaaTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAagag                                                                                                                       >  1:672029/1‑140 (MQ=255)                  aaTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCg                                                                                                                   <  1:605732/140‑1 (MQ=255)                   aTTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGa                                                                                                                  <  2:557167/140‑1 (MQ=255)                         cAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCa                                                                                                            <  2:44692/140‑1 (MQ=255)                           aaTCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCATACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCaaa                                                                                                          <  1:323771/140‑1 (MQ=255)                              cgctcgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGTAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGc                                                                                                       <  2:672029/140‑1 (MQ=255)                               gctcgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCa                                                                                                      <  2:727658/140‑1 (MQ=255)                               gctcgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCa                                                                                                      <  1:873800/140‑1 (MQ=255)                                 tcgcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAg                                                                                                    <  2:434232/140‑1 (MQ=255)                                   gcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTc                                                                                                  <  2:490291/140‑1 (MQ=255)                                   gcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTc                                                                                                  <  2:539825/140‑1 (MQ=255)                                   gcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTc                                                                                                  <  2:546930/140‑1 (MQ=255)                                   gcCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTc                                                                                                  <  1:692896/140‑1 (MQ=255)                                           cACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACg                                                                                          <  1:589592/140‑1 (MQ=255)                                             cGAATCAATGCCTAAAGGAATCAGGGAGTTCGTGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTc                                                                                                  <  1:606883/130‑1 (MQ=255)                                             cGAATCAATGCCTAAAGGAATCAGGGAGTTCGTGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTc                                                                                                  >  2:606883/1‑130 (MQ=255)                                             cGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGa                                                                                        <  1:635778/140‑1 (MQ=255)                                             cGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGa                                                                                        <  1:936978/140‑1 (MQ=255)                                                  cAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGt                                                                                   >  2:626360/1‑140 (MQ=255)                                                          aaaGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACt                                                                           >  1:391132/1‑140 (MQ=255)                                                          aaaGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACt                                                                           >  2:373950/1‑140 (MQ=255)                                                              gAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGgcg                                                                       <  1:334792/140‑1 (MQ=255)                                                              gAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGgcg                                                                       <  1:459184/140‑1 (MQ=255)                                                               aaTCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGACAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGgcgc                                                                      >  1:778383/1‑140 (MQ=255)                                                                aTCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCgct                                                                     >  1:92405/1‑140 (MQ=255)                                                                 tCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCgctg                                                                    >  1:79382/1‑140 (MQ=255)                                                                     ggAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGATAAACTGGCGCTGCTTg                                                                <  2:977833/140‑1 (MQ=255)                                                                     ggAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTg                                                                >  1:801757/1‑140 (MQ=255)                                                                       aGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGaa                                                              <  2:922888/140‑1 (MQ=255)                                                                         ttCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACa                                                            >  2:949731/1‑140 (MQ=255)                                                                         ttCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACa                                                            >  2:341511/1‑140 (MQ=255)                                                                          tCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACaa                                                           >  1:348240/1‑140 (MQ=255)                                                                                   aTGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGc                                                  >  2:888257/1‑140 (MQ=255)                                                                                     gtgGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCATACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGa                                                >  1:56397/1‑140 (MQ=255)                                                                                        gagaAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACt                                             >  2:572218/1‑140 (MQ=255)                                                                                            aaaaaaTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGaa                                         <  1:292420/140‑1 (MQ=255)                                                                                            aaaaaaTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGaa                                         <  2:961075/140‑1 (MQ=255)                                                                                              aaaaTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGaaaa                                       <  1:572218/140‑1 (MQ=255)                                                                                                 aTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCg                                    <  2:337655/140‑1 (MQ=255)                                                                                                 aTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCg                                    <  2:525863/140‑1 (MQ=255)                                                                                                 aTCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCg                                    <  1:888257/140‑1 (MQ=255)                                                                                                   ccGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCa                                  <  2:750784/140‑1 (MQ=255)                                                                                                   ccGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCa                                  <  2:92405/140‑1 (MQ=255)                                                                                                     gCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGt                                >  2:94003/1‑140 (MQ=255)                                                                                                     gCCAAACCCTACAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGt                                >  2:120743/1‑140 (MQ=255)                                                                                                                cAAGCGCAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCaa                     >  1:419411/1‑140 (MQ=255)                                                                                                                      cAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagca               >  1:407517/1‑140 (MQ=255)                                                                                                                      cAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagca               >  1:793898/1‑140 (MQ=255)                                                                                                                      cAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagca               >  2:764162/1‑140 (MQ=255)                                                                                                                      cAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagca               >  1:817405/1‑140 (MQ=255)                                                                                                                      cAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagca               <  2:801757/140‑1 (MQ=255)                                                                                                                      cAGCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagca               >  2:887910/1‑140 (MQ=255)                                                                                                                        gCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagcagc             >  2:759156/1‑140 (MQ=255)                                                                                                                        gCTGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAagcagc             <  2:592094/140‑1 (MQ=255)                                                                                                                          tGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAAGCAGCCa           <  2:817405/140‑1 (MQ=255)                                                                                                                          tGACGCGCCTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAAGCAGCCa           <  1:341511/140‑1 (MQ=255)                                                                                                                                 ccTCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAAGCAGCCAGATCCag    >  1:252492/1‑140 (MQ=255)                                                                                                                                   tCGGCCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAAGCAGCCAGATCCagag  <  2:940300/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                         ATGATTGACCCGAAAAAAATTGAGCAAATCGCTCGCCAGGTTCACGAATCAATGCCTAAAGGAATCAGGGAGTTCGGGGAAGATGTGGAGAAAAAAATCCGCCAAACCCTACAAGCGCAGCTGACGCGCCTCGACCTTGTAAGCCGTGAAGAGTTCGACGTCCAAACGCAAGTCCTGTTACGGACTCGTGAAAAACTGGCGCTGCTTGAACAAAGAATCAGCGAACTGGAAAATCGCAGTACTGAGATCAAAAAGCAGCCAGATCCAGAG  >  NC_000913/3184840‑3185109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑