Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,941,916	A→G	noncoding (109/1542 nt)	rrsC →	16S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,941,916	0	A	G	100.0%	16.9 / NA	11	noncoding (109/1542 nt)	rrsC	16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/8);  total (3/8)

TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAT  >  NC_000913/3941877‑3942051                                        |                                                                                                                                        tAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCtt                                     <  2:553978/140‑1 (MQ=14)  aaCAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGGCGGGGGAGTAATGTCTGGGAAACTGCCTGATGGGGGGGGGTAAATACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAAAGGGGGGCCttt                                    >  2:831240/1‑139 (MQ=11)   aCAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCg                                   <  1:660469/140‑1 (MQ=18)        aaaCAGCTTGCTGTTTCGCTGACGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCct                              <  2:743146/140‑1 (MQ=18)          aCAGCTTGCTGTTTCGCTGACGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCctct                            <  1:150808/140‑1 (MQ=17)              cTTGCTGTTTCGCTGACGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGcc                        <  1:564234/140‑1 (MQ=18)                       tCGCTGACGAGTGGCGGGCGGGGGGGTAATGTCTGGGGAACTGCCTGATGGAGGGGGGTAACTACTGGAAAAGGTAGCTAATACCGCATAACGTCGCAAAACCAAAGAGGGGGGCCTTCGGGCCTCTTGCCATCAGAtgg               >  2:52607/1‑139 (MQ=11)                          cTGACGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGcc            <  2:731738/140‑1 (MQ=18)                              cGAGTGGCGGGCGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa        <  1:608237/140‑1 (MQ=18)                              cGAGTGGCGGGCGGGTGAGTAATGTCTAGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGCCCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGa        <  1:604433/140‑1 (MQ=12)                               gAGTGGCGGGCGGGGGAGTAATGTCTGGGAAAATGCCTGGTGGAGGGGGGTAACTACTGGAAAAGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGGCCTTCGGGGCCCTTGCCATCAGATGTGCCCCGaa       >  2:383562/1‑139 (MQ=11)                                   ggcgggcgGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGa   <  1:831240/140‑1 (MQ=18)                                   ggcgggcgGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGa   <  1:850214/140‑1 (MQ=17)                                    gcgggcggGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAt  <  1:406246/140‑1 (MQ=18)                                        |                                                                                                                                        TAACAGGAAACAGCTTGCTGTTTCGCTGACGAGTGGCGGACGGGTGAGTAATGTCTGGGAAACTGCCTGATGGAGGGGGATAACTACTGGAAACGGTAGCTAATACCGCATAACGTCGCAAGACCAAAGAGGGGGACCTTCGGGCCTCTTGCCATCAGATGTGCCCAGATGGGAT  >  NC_000913/3941877‑3942051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑