Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,182,928	G→A	N231N (AAC→AAT)	potC ←	spermidine preferential ABC transporter membrane subunit PotC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,182,928	0	G	A	100.0%	119.6 / NA	34	N231N (AAC→AAT)	potC	spermidine preferential ABC transporter membrane subunit PotC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

CCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCG  >  NC_000913/1182795‑1183064                                                                                                                                      |                                                                                                                                         cccGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACt                                                                                                                                    >  2:360511/1‑140 (MQ=255)                         cATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGAcc                                                                                                            <  1:786113/140‑1 (MQ=255)                                tCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAAt                                                                                                     <  2:405294/140‑1 (MQ=255)                                tCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAAt                                                                                                     <  1:384001/140‑1 (MQ=255)                                             ccTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACgg                                                                                        <  1:835290/140‑1 (MQ=255)                                                gtgtTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAg                                                                                     <  1:725240/140‑1 (MQ=255)                                                 tgtTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGa                                                                                    <  1:264834/140‑1 (MQ=255)                                                     aCCTTTCGTTTTATCAC‑‑GCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCa                                                                              >  2:270987/1‑140 (MQ=255)                                                      ccTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTc                                                                               >  2:846183/1‑140 (MQ=255)                                                           cGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACgg                                                                                        <  2:4298/126‑1 (MQ=255)                                                           cGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACgg                                                                                        >  1:4298/1‑126 (MQ=255)                                                                tATCACGAGCAATAAGCTGGCTGGCAATGACGATCACTAGCGAGAGCACCAGGAATATGGTTGCGAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAAGGTCATaagtgcgg                                                                     >  2:947858/1‑134 (MQ=255)                                                                     cGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGt                                                                <  1:846183/140‑1 (MQ=255)                                                                                 tggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACgaaga                                                    <  2:8568/140‑1 (MQ=255)                                                                                 tggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACgaaga                                                    <  2:414350/140‑1 (MQ=255)                                                                                 tggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACgaaga                                                    <  1:183945/140‑1 (MQ=255)                                                                                 tggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACgaaga                                                    <  1:947858/140‑1 (MQ=255)                                                                                 tggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACgaaga                                                    <  1:360511/140‑1 (MQ=255)                                                                                 tggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACgaaga                                                    <  2:591259/140‑1 (MQ=255)                                                                                  ggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGAcgaacgaa                                                      >  2:809750/1‑137 (MQ=255)                                                                                  ggctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGAcgaacgaa                                                      <  1:809750/137‑1 (MQ=255)                                                                                    ctggcAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAAc                                                 >  2:597462/1‑140 (MQ=255)                                                                                                 tCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACcaca                                          <  1:907861/134‑1 (MQ=255)                                                                                                 tCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACcaca                                          >  2:907861/1‑134 (MQ=255)                                                                                                 tCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTcc                                    >  1:772240/1‑140 (MQ=255)                                                                                                  cACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCa                                   >  1:838358/1‑140 (MQ=255)                                                                                                   aCCAGCGACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCAt                                  <  2:112294/140‑1 (MQ=255)                                                                                                         gACAGCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAg                            >  1:919898/1‑140 (MQ=255)                                                                                                             gCACCAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTa                        <  2:888457/140‑1 (MQ=255)                                                                                                                 cAGTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGc                    >  1:221241/1‑140 (MQ=255)                                                                                                                   gTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACa                             >  2:28578/1‑129 (MQ=255)                                                                                                                   gTAATATGGTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACa                             <  1:28578/129‑1 (MQ=255)                                                                                                                          ggTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACAccc           >  1:48815/1‑140 (MQ=255)                                                                                                                           gTTGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCa          >  1:728525/1‑140 (MQ=255)                                                                                                                             tGCCAGCGCATTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGc        >  1:363616/1‑140 (MQ=255)                                                                                                                                   cgcaTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCccgccg  >  2:261536/1‑140 (MQ=255)                                                                                                                                      |                                                                                                                                         CCCGCCGCGAGCAGGTGGCGTGACCATTTTTTCATTTAACGTCCCCTGTGTTACCTTTCGTTTTATCACGAGCAATAAGCTGGCTGGCAATAACCATCACCAGCGACAGCACCAGTAATATGGTTGCCAGCGCGTTAACTTCCGGCGATACGCCGACTTTGACCATCGAATAAATTTTTAACGGCAGAATTTCATAACTTGGTCCGGTGACGAACGAAGAAACTACCACATCGTCCATCGACAGGGTAAAGCTTAACACCCAGCCCGCCG  >  NC_000913/1182795‑1183064

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑