Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	369,030	G→C	E140D (GAG→GAC)	mhpA →	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	369,030	0	G	C	100.0%	164.3 / NA	42	E140D (GAG→GAC)	mhpA	putative 3‑(3‑hydroxyphenyl)propanoate/3‑hydroxycinnamate hydroxylase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (21/21);  total (21/21)

AGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGC  >  NC_000913/368909‑369156                                                                                                                          |                                                                                                                               aGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGTGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTtcagtcag                                                                                                              >  2:512233/1‑140 (MQ=255)   ccAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCa                                                                                                            >  2:454028/1‑140 (MQ=255)   ccAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCa                                                                                                            <  2:11220/140‑1 (MQ=255)   ccAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCa                                                                                                            >  2:567144/1‑140 (MQ=255)   ccAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCa                                                                                                            <  1:459372/140‑1 (MQ=255)     aaTGACCGATGAATTTGGCTGGCCGC‑CCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGa                                                                                                         >  1:109880/1‑140 (MQ=255)       tGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGAt                                                                                                        <  1:666129/140‑1 (MQ=255)             aTGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGaa                                                                                                  >  2:248993/1‑140 (MQ=255)             aTGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGaa                                                                                                  <  1:53177/140‑1 (MQ=255)             aTGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGaa                                                                                                  >  2:53177/1‑140 (MQ=255)                    tggctggcCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCt                                                                                           >  1:386752/1‑140 (MQ=255)                     ggctggcCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCtt                                                                                          >  1:122156/1‑140 (MQ=255)                       ctggcCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGc                                                                                        >  1:247830/1‑140 (MQ=255)                          gcCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCAcc                                                                                     >  1:709958/1‑140 (MQ=255)                            cgcgCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGGCCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTg                                                                                   <  2:446650/140‑1 (MQ=255)                                           ttATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAggg                                                                    <  1:278935/140‑1 (MQ=255)                                               tCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGc                                                                <  2:47167/140‑1 (MQ=255)                                                   ccGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGa                                                            >  2:3368/1‑140 (MQ=255)                                                   ccGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGa                                                            >  2:689374/1‑140 (MQ=255)                                                      cAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAAt                                                         <  2:122156/140‑1 (MQ=255)                                                            gATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCaa                                                   <  1:42493/140‑1 (MQ=255)                                                            gATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCaa                                                   <  2:109880/140‑1 (MQ=255)                                                                  gTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCa                                             <  2:234018/140‑1 (MQ=255)                                                                    gATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGt                                           <  2:145939/140‑1 (MQ=255)                                                                        cTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCt                                       >  2:185924/1‑140 (MQ=255)                                                                          ggAAGGGGTGTCGCGTTTTCCAAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTgg                                     <  1:129538/140‑1 (MQ=255)                                                                            aaGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTa                                   >  2:153841/1‑140 (MQ=255)                                                                                ggTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCt                               >  1:426263/1‑140 (MQ=255)                                                                                    tCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGa                           >  2:503031/1‑140 (MQ=255)                                                                                       cgTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACgg                        >  1:498505/1‑140 (MQ=255)                                                                                            tCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAg                   <  2:426263/140‑1 (MQ=255)                                                                                            tCCGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAg                   <  1:503031/140‑1 (MQ=255)                                                                                             ccGAATGTGCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGc                  <  1:689374/140‑1 (MQ=255)                                                                                                    tgCGCTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGtttt           >  2:562203/1‑140 (MQ=255)                                                                                                      cgcTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGt         >  1:9485/1‑140 (MQ=255)                                                                                                      cgcTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGt         <  2:247830/140‑1 (MQ=255)                                                                                                      cgcTGCTTGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGt         >  1:221388/1‑140 (MQ=255)                                                                                                            ttGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCg   <  1:567144/140‑1 (MQ=255)                                                                                                            ttGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCg   <  1:454028/140‑1 (MQ=255)                                                                                                            ttGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCg   <  1:185924/140‑1 (MQ=255)                                                                                                             tGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGc  >  1:564186/1‑140 (MQ=255)                                                                                                             tGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGc  <  1:153841/140‑1 (MQ=255)                                                                                                             tGTTTTCCCGCGACCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGc  <  2:9485/140‑1 (MQ=255)                                                                                                                          |                                                                                                                               AGCCAATGACCGATGAATTTGGCTGGCCGCGCCGTAACGCCTTTATTCAGCCGCAGGTCGATGCGGTGATGCTGGAAGGGGTGTCGCGTTTTCCGAATGTGCGCTGCTTGTTTTCCCGCGAGCTGGAGGCCTTCAGTCAGCAAGATGACGAAGTGACCTTGCACCTGAAAACGGCAGAAGGGCAGCGGGAAATAGTCAAAGCCCAGTGGCTGGTAGCCTGTGACGGTGGAGCAAGTTTTGTCCGTCGC  >  NC_000913/368909‑369156

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑