Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,027,161	G→A	Q205* (CAA→TAA)	yccT ←	DUF2057 domain‑containing protein YccT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,027,161	0	G	A	100.0%	133.0 / NA	38	Q205* (CAA→TAA)	yccT	DUF2057 domain‑containing protein YccT
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/23);  total (15/23)

CCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGC  >  NC_000913/1027025‑1027291                                                                                                                                         |                                                                                                                                   ccGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATgg                                                                                                                                 >  1:589887/1‑140 (MQ=255)       ttAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGc                                                                                                                           <  1:179191/140‑1 (MQ=255)       ttAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGc                                                                                                                           <  1:212252/140‑1 (MQ=255)         aaCTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCa                                                                                                                         >  2:456404/1‑140 (MQ=255)                               aGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCg                                                                                                   >  2:140107/1‑140 (MQ=255)                                  aCTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCgttg                                                                                                >  1:582653/1‑140 (MQ=255)                                     tATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTc                                                                                             >  2:393367/1‑140 (MQ=255)                                       tCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCtg                                                                                           >  2:489840/1‑140 (MQ=255)                                       tCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCtg                                                                                           >  1:3191/1‑140 (MQ=255)                                        cGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCtgt                                                                                          <  1:26564/140‑1 (MQ=255)                                          cTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGa                                                                                        <  2:272376/140‑1 (MQ=255)                                           tGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAg                                                                                       >  1:523973/1‑140 (MQ=255)                                            gCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGc                                                                                      >  1:58668/1‑140 (MQ=255)                                            gCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGc                                                                                      <  1:32436/140‑1 (MQ=255)                                              aaGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCAc                                                                                    >  2:370005/1‑140 (MQ=255)                                                       aCCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTg                                                                           <  2:589887/140‑1 (MQ=255)                                                       aCCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTg                                                                           <  2:3191/140‑1 (MQ=255)                                                           aaaaaGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGt                                                                       >  1:208833/1‑140 (MQ=255)                                                                gCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAAt                                                                  >  2:577216/1‑140 (MQ=255)                                                                     cgcACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAACGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCAt                                                             <  2:582653/140‑1 (MQ=255)                                                                     cgcACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCAt                                                             >  2:332619/1‑140 (MQ=255)                                                                      gcACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATc                                                            <  1:393367/140‑1 (MQ=255)                                                                      gcACAGGCACAAAAATCTCAGGAAGATGGTTGTTTCTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATc                                                            <  2:448301/140‑1 (MQ=255)                                                                                 aaaaTCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGaaa                                                 <  1:370005/140‑1 (MQ=255)                                                                                      ctcAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACAccc                                            <  2:652761/140‑1 (MQ=255)                                                                                        cAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGa                                          <  2:58668/140‑1 (MQ=255)                                                                                          ggAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGaaa                                        <  2:403361/140‑1 (MQ=255)                                                                                             aGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAagca                                     <  1:461617/140‑1 (MQ=255)                                                                                                  gttgttTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTa                                <  2:540593/140‑1 (MQ=255)                                                                                                      tttttCCGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGt                            <  1:456404/140‑1 (MQ=255)                                                                                                            cGCCCACTGCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTg                      <  2:390082/140‑1 (MQ=255)                                                                                                                    gCAGGAAAGTATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCatcatc              <  1:489840/140‑1 (MQ=255)                                                                                                                             tATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAAt     <  2:285384/140‑1 (MQ=255)                                                                                                                             tATTTCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAAt     >  2:232358/1‑140 (MQ=255)                                                                                                                               tttCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTg   <  2:442079/140‑1 (MQ=255)                                                                                                                               tttCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTg   <  1:618894/140‑1 (MQ=255)                                                                                                                               tttCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTg   <  1:140107/140‑1 (MQ=255)                                                                                                                                ttCGCGTTTATGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGc  >  1:248783/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   CCGTAGTTAACTTTCCTACAGATTACTGTAAGCACTTATCGCTGCAAGATAAAGACCGAAAAAGCCTGCGCACAGGCACAAAAATCTCAGGAAGATGGTTGTTTTTCCGCCCACTGCAGGAAAGTATTTCGCGTTTGTGGGTCAGCCAGTTTAAACCAATACTTCAGCCGTTGTTCTGTGAGCACCTGAGACTGCGGTGGAATAGCATCCAGCTCGGAAACACCCGAAAGCAGCGTACTGTCATCTGCCATCATCGTGGCAAATTGC  >  NC_000913/1027025‑1027291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑