Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT41.7% 15.5 / 19.7 24intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/14);  new base T (10/0);  total (10/14)
Fisher's exact test for biased strand distribution p-value = 5.10e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTG  >  NC_000913/3206298‑3206564
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aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                 <  2:447139/140‑1 (MQ=255)
    cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACa                                                                                                                             <  2:509189/140‑1 (MQ=255)
              cccTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAcaaaaacaaaaa                                                                                                                   >  1:161415/1‑140 (MQ=255)
                   ctctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGAaaaaaaaaaaaaaaaga                                                                                                              >  2:198903/1‑135 (MQ=255)
                    tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGTTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                             <  2:346992/140‑1 (MQ=255)
                    tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                             <  1:113883/140‑1 (MQ=255)
                        gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTTAGACAAAAACAAAAAAAGGAGGTgg                                                                                                         >  2:681344/1‑139 (MQ=255)
                         cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGcc                                                                                                        >  1:588334/1‑140 (MQ=255)
                             atatTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatga                                                                                                    <  2:197123/140‑1 (MQ=255)
                                        tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGTTGCCATGATGAGCGGAAgg                                                                                         >  1:383784/1‑139 (MQ=255)
                                             tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                    <  1:198903/140‑1 (MQ=255)
                                             tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                    <  2:18399/140‑1 (MQ=255)
                                                  ttGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAAc                                                                               <  1:277531/140‑1 (MQ=255)
                                                     aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                            <  1:681344/140‑1 (MQ=255)
                                                                          tatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAgaga                                                       <  1:620898/140‑1 (MQ=255)
                                                                           atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCACGCAAAAACAAAAAATGGGGGTGCCCTTATTGGCCAAAATAATAAACAACAAGCAGAGGATAAGTTGGAAGAGAt                                                      >  2:601618/1‑140 (MQ=255)
                                                                              cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGCCATGATGAGGGAAAGGAAAAAACAAAAAGCAGTGAAAAAATTGACAAAGATTGt                                                   >  2:299455/1‑140 (MQ=255)
                                                                                     gCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAGGAAAAAAAAAAAGGCAATGAAAAAAATGACAGAGAATTTCGCTaaa                                            >  2:140426/1‑139 (MQ=255)
                                                                                         aaaaTATAAACCCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                        <  1:140426/140‑1 (MQ=255)
                                                                                           aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCAGACAAAAAAAAAAAAAGGAGTTGCCATTATGAGGGAAAAGAAAAAACAAAAAGCAGAGAAAAAGATTACAGAGAATGTCGCTAAATTTatc                                      >  2:599526/1‑138 (MQ=255)
                                                                                             tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGCCCTTATGAGCGAAAGGAATAAAAAAAAAGCAGTGAATAAATTGACAGAGATTGTCGCTAACTTTACCGc                                    >  2:179555/1‑140 (MQ=255)
                                                                                               taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                  <  1:179555/140‑1 (MQ=255)
                                                                                               taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAAAAAAAAAAATGGGGGTTCCCAGATGAGCGGAAGAAAAAAAAAAAAAGCAGTGAAAAAATTGACAGAGATTGTCGCTAAATTTACCGccc                                  >  2:182529/1‑139 (MQ=255)
                                                                                               aaaaaCCACATTTTTAGTGTTAGTTTGTGGCGGTGATTTTTTCCAGACAAATAAAAAAACGGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAATTTTACCGCCa                                  <  2:161415/139‑1 (MQ=255)
                                                                                                                  gTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGcc               <  1:434826/140‑1 (MQ=255)
                                                                                                                  gTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAAATGGAGGTGGCATGATGAGCGGAAGTAAAAAGCAAAAGGGAGTGAAAAAAATTAAAGAGATTTTCGCTAACTTTTACGCCACGATTTTTTCCCGAAAGcc               >  2:434826/1‑140 (MQ=255)
                                                                                                                           ggCGGTGGATTTTTTCCGACAAAAAAAAAAAATAGAGGTGGCCTGATGAGGGGAAGAAAAAAACAAAAGGCAGGGAATAAGTTGACAGAGATTTTTGCTAACTTTTTCGCCATGATTTTTTCCCCAAAGCCCGGTGACgg      >  2:509732/1‑139 (MQ=255)
                                                                                                                            gCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtg     <  1:299455/140‑1 (MQ=255)
                                                                                                                               gTGAATTTTTTCCGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGGAATAAACAAAAAGCAGTGAATAAGATGACAGAGAATGTCGCTAACTTTAACCCCCTGATTTCTACCCGCATGCCTGATGACgtggtg  >  2:398161/1‑140 (MQ=255)
                                                                                                                               gTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATGAGCGGAAGGAATAAGCAACAGGCAGTGAAAAAGTTGACAGAGAATTTCGCTAAATTTACCGCCATGATTTCTACCCCAATGCCTGACGACgtggtg  >  2:212728/1‑140 (MQ=255)
                                                                                                                                         |                                                                                                                                 
AAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTG  >  NC_000913/3206298‑3206564

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑