Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,513,186	T→C	L124L (TTG→CTG)	ydcT →	putative ABC transporter ATP‑binding protein YdcT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,513,186	0	T	C	100.0%	114.8 / NA	30	L124L (TTG→CTG)	ydcT	putative ABC transporter ATP‑binding protein YdcT
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (16/14);  total (16/14)

AATACTGTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGG  >  NC_000913/1513051‑1513312                                                                                                                                        |                                                                                                                               aaTACTGTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTggg                                                                                                                            >  1:151180/1‑140 (MQ=255) aaTACTGTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTggg                                                                                                                            >  2:610151/1‑140 (MQ=255) aaTACTGTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTggg                                                                                                                            <  1:643148/140‑1 (MQ=255)          tttCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACa                                                                                                                   >  2:693040/1‑140 (MQ=255)                     cgcgCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTa                                                                                                                     >  2:94396/1‑127 (MQ=255)                     cgcgCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTa                                                                                                                     <  1:94396/127‑1 (MQ=255)                                      tGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCtggt                                                                                       >  1:417004/1‑140 (MQ=255)                                      tGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCtggg                                                                                       >  1:630610/1‑139 (MQ=255)                                        tCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCtggtgg                                                                                     >  1:22509/1‑140 (MQ=255)                                        tCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCtggtgg                                                                                     <  2:417004/140‑1 (MQ=255)                                           aTTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGCGGCGCTGGGGTTTGTACATCAACGTAAACCGTCCCAACTTTCTGGTGGTCa                                                                                  >  2:393130/1‑140 (MQ=255)                                                 gACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCa                                                                            >  1:610113/1‑140 (MQ=255)                                                  aCAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAg                                                                           >  2:486372/1‑140 (MQ=255)                                                                gggCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGc                                                             <  1:486372/140‑1 (MQ=255)                                                                  gCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCa                                                           <  2:206356/140‑1 (MQ=255)                                                                       tGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCa                                                      <  2:610113/140‑1 (MQ=255)                                                                         gTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCAtt                                                    >  2:46653/1‑140 (MQ=255)                                                                               ggCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaa                                              <  1:610151/140‑1 (MQ=255)                                                                                   tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaatgaa                                          >  1:406548/1‑140 (MQ=255)                                                                                   tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGtgaatgaa                                          >  1:29/1‑140 (MQ=255)                                                                                   tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCAAGCGGGTTGCTATCGCCAGAGCATTGGtgaatgaa                                          >  2:128830/1‑140 (MQ=255)                                                                                   tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTAAGCGCCAGCGGGTTGCTATCGCCAGAGCAtt                                                    >  1:352678/1‑130 (MQ=255)                                                                                   tGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTAAGCGCCAGCGGGTTGCTATCGCCAGAGCAtt                                                    <  2:352678/130‑1 (MQ=255)                                                                                      aTAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcg                                       >  1:363546/1‑140 (MQ=255)                                                                                       tAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACcgc                                      <  2:341375/140‑1 (MQ=255)                                                                                            agcagcGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTa                                 <  2:22509/140‑1 (MQ=255)                                                                                                  ggCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTg                           <  1:46653/140‑1 (MQ=255)                                                                                                                cAAGAGGCGCTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCt             <  2:406548/140‑1 (MQ=255)                                                                                                                       cgcTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCa      >  1:487191/1‑140 (MQ=255)                                                                                                                         cTGGAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCAAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACt    <  1:128830/140‑1 (MQ=255)                                                                                                                           ggAGAAAGTGGCGCTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTgg  <  1:693040/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                               AATACTGTCTTTCAGGACTACGCGCTATTTCCGCATATGTCGATTCTTGACAATGTCGCCTATGGGCTGATGGTCAAAGGCGTGAATAAAAAGCAGCGGCACGCAATGGCGCAAGAGGCGCTGGAGAAAGTGGCGTTGGGGTTTGTACATCAACGTAAACCGTCACAACTTTCTGGTGGTCAGCGCCAGCGGGTTGCTATCGCCAGAGCATTGGTGAATGAACCGCGCGTATTGCTGTTGGATGAACCGCTCGGCGCACTGG  >  NC_000913/1513051‑1513312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑