Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,678,988	A→C	I188L (ATC→CTC)	ydgH →	DUF1471 domain‑containing protein YdgH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,678,988	0	A	C	100.0%	133.4 / NA	35	I188L (ATC→CTC)	ydgH	DUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/16);  total (19/16)

CAAAAGCGAAAGGTGCCTACTCTTTCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAG  >  NC_000913/1678857‑1679119                                                                                                                                    |                                                                                                                                    cAAAAGCGAAAGGTGCCTACTCTTTCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCa                                                                                                                             >  2:130573/1‑140 (MQ=255)               gCCTACTCTTTCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAgg                                                                                                               <  1:130573/140‑1 (MQ=255)               gCCTACTCTTTCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAgg                                                                                                               <  1:380706/140‑1 (MQ=255)                       tttCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAg                                                                                                       >  2:445294/1‑140 (MQ=255)                        ttCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGc                                                                                                      >  1:283644/1‑140 (MQ=255)                                 gttcgtCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGc                                                                                             >  2:237444/1‑140 (MQ=255)                                  ttcgtCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGcc                                                                                            >  2:521992/1‑140 (MQ=255)                                                   aaCCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCgaa                                                                           <  1:445294/140‑1 (MQ=255)                                                     ccAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCgaaga                                                                         >  2:120079/1‑140 (MQ=255)                                                             gcAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTgag                                                                 >  1:37125/1‑140 (MQ=255)                                                             gcAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTgag                                                                 <  1:237444/140‑1 (MQ=255)                                                              cAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGtt                                                                    >  1:144962/1‑136 (MQ=255)                                                              cAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGtt                                                                    <  2:144962/136‑1 (MQ=255)                                                                aCCAGCGTATTATTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATc                                                              >  2:432479/1‑140 (MQ=255)                                                                    gCGTATTATTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCgg                                                          <  1:432479/140‑1 (MQ=255)                                                                    gCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCgg                                                          >  2:504280/1‑140 (MQ=255)                                                                          tACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTg                                                    >  2:505108/1‑140 (MQ=255)                                                                          tACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTg                                                    >  1:266289/1‑140 (MQ=255)                                                                            cTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCg                                                  >  2:513745/1‑140 (MQ=255)                                                                                          aaaaaaGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCAcc                                    <  1:521992/140‑1 (MQ=255)                                                                                          aaaaaaGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCAcc                                    <  1:505108/140‑1 (MQ=255)                                                                                          aaaaaaGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCAcc                                    <  2:37125/140‑1 (MQ=255)                                                                                          aaaaaaGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCAcc                                    <  2:263646/140‑1 (MQ=255)                                                                                              aaGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGt                                >  1:290008/1‑140 (MQ=255)                                                                                                    cTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGAT‑CCGGGTGTTGCGACTACCGCATCAcc                                    <  2:529461/129‑1 (MQ=255)                                                                                                    cTAAGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGAT‑CCGGGTGTTGCGACTACCGCATCAcc                                    >  1:529461/1‑129 (MQ=255)                                                                                                       aGAAACGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAgtc                       <  1:120079/140‑1 (MQ=255)                                                                                                           aCGTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAgtcggtc                   >  2:525254/1‑140 (MQ=255)                                                                                                             gTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGc                 <  2:213054/140‑1 (MQ=255)                                                                                                             gTATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGc                 >  1:215570/1‑140 (MQ=255)                                                                                                              tATCGTCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGct                <  1:513745/140‑1 (MQ=255)                                                                                                                   tCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGctt               <  2:242589/136‑1 (MQ=255)                                                                                                                   tCCAGAGCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGctt               >  1:242589/1‑136 (MQ=255)                                                                                                                       gagCCCGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGaaa       >  2:104100/1‑140 (MQ=255)                                                                                                                            cGGATGTGCTCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAg  <  2:283644/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    CAAAAGCGAAAGGTGCCTACTCTTTCTACATCGTTCGTCAAATCGATGCCAACCAGGGCGGCAACCAGCGTATTACTGCATTCATCTATAAAAAAGATGCTAAGAAACGTATCGTCCAGAGCCCGGATGTGATCCCGGCAGATTCCGAAGCAGGACGTGCAGCTCTGGCTGCCGGTGGCGAAGCCGCGAAGAAAGTTGAGATCCCGGGTGTTGCGACTACCGCATCACCAAGTTCTGAAGTCGGTCGCTTCTTTGAAACCCAG  >  NC_000913/1678857‑1679119

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑