Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	637,138	G→T	R161S (CGT→AGT)	ybdO ←	putative LysR family DNA‑binding transcriptional regulator YbdO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	637,138	0	G	T	100.0%	220.5 / NA	63	R161S (CGT→AGT)	ybdO	putative LysR family DNA‑binding transcriptional regulator YbdO
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (36/27);  total (36/27)

TGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACGGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGATCAGC  >  NC_000913/637001‑637274                                                                                                                                          |                                                                                                                                         tgctgATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGc                                                                                                                                        >  1:654947/1‑140 (MQ=255)  gctgATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCt                                                                                                                                       >  1:137391/1‑140 (MQ=255)      aTTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGAt                                                                                                                                    <  1:358177/139‑1 (MQ=255)      aTTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGAt                                                                                                                                    >  2:358177/1‑139 (MQ=255)      aTTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATc                                                                                                                                   >  2:244598/1‑140 (MQ=255)       tttAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCa                                                                                                                                  >  1:72366/1‑140 (MQ=255)        ttAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCAc                                                                                                                                 >  2:461884/1‑140 (MQ=255)        ttAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCAc                                                                                                                                 >  2:267749/1‑140 (MQ=255)                tAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCAttt                                                                                                                         >  1:394031/1‑140 (MQ=255)                    tGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGt                                                                                                                     <  2:654947/140‑1 (MQ=255)                       gTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGAt                                                                                                                  >  1:377816/1‑140 (MQ=255)                         gAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCa                                                                                                                >  1:463302/1‑140 (MQ=255)                         gAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCa                                                                                                                <  2:682213/140‑1 (MQ=255)                         gAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCATATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCa                                                                                                                >  2:348122/1‑140 (MQ=255)                               ttctGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCATATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGAt                                                                                                          <  1:348122/140‑1 (MQ=255)                                 ctGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCg                                                                                                        >  1:114739/1‑140 (MQ=255)                                    ccATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCt                                                                                                     >  1:646012/1‑140 (MQ=255)                                           ttGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGt                                                                                              <  2:394031/140‑1 (MQ=255)                                           ttGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGt                                                                                              <  2:72366/140‑1 (MQ=255)                                              ttCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGAt                                                                                                          <  1:105221/125‑1 (MQ=255)                                              ttCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGAt                                                                                                          >  2:105221/1‑125 (MQ=255)                                                     gTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAg                                                                                    <  2:377816/140‑1 (MQ=255)                                                        cTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGttc                                                                                 <  2:137391/140‑1 (MQ=255)                                                          aTTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGttctt                                                                               <  2:38928/140‑1 (MQ=255)                                                                aGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCAt                                                                         >  2:448671/1‑140 (MQ=255)                                                                        gTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTctgct                                                                 >  2:328333/1‑140 (MQ=255)                                                                                cTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAg                                                         <  1:448671/140‑1 (MQ=255)                                                                                         cAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATg                                                <  1:461884/140‑1 (MQ=255)                                                                                          aaaTTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATgg                                               >  2:138148/1‑140 (MQ=255)                                                                                             ttAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCAc                                            <  2:646012/140‑1 (MQ=255)                                                                                             ttAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCAc                                            >  2:499440/1‑140 (MQ=255)                                                                                                 agTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCaa                                        >  2:305237/1‑140 (MQ=255)                                                                                                 agTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCaa                                        >  2:505971/1‑140 (MQ=255)                                                                                                 agTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCaa                                        >  1:66224/1‑140 (MQ=255)                                                                                                      tGCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCg                                   >  1:511182/1‑140 (MQ=255)                                                                                                       gCGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGa                                  <  1:244598/140‑1 (MQ=255)                                                                                                        cGAATCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGAc                                 >  1:561472/1‑140 (MQ=255)                                                                                                           aTCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACaga                              >  1:496262/1‑140 (MQ=255)                                                                                                           aTCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACaga                              >  2:413748/1‑140 (MQ=255)                                                                                                            tCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACagag                             <  1:305237/140‑1 (MQ=255)                                                                                                            tCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACagag                             <  1:328333/140‑1 (MQ=255)                                                                                                            tCGTATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACagag                             <  2:463302/140‑1 (MQ=255)                                                                                                               tATGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGaa                          >  2:386314/1‑140 (MQ=255)                                                                                                                 tGCAATGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATc                        <  1:138148/140‑1 (MQ=255)                                                                                                                    aaTGGCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTg                     >  1:193551/1‑140 (MQ=255)                                                                                                                       ggCATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCg                  >  1:609855/1‑140 (MQ=255)                                                                                                                         cATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTa                >  1:287563/1‑140 (MQ=255)                                                                                                                         cATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTa                >  1:207405/1‑140 (MQ=255)                                                                                                                         cATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTa                >  1:558253/1‑140 (MQ=255)                                                                                                                         cATGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTa                >  2:216987/1‑140 (MQ=255)                                                                                                                           tGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAgg              >  2:530885/1‑140 (MQ=255)                                                                                                                           tGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAgg              <  1:499440/140‑1 (MQ=255)                                                                                                                           tGCAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAgg              >  1:34074/1‑140 (MQ=255)                                                                                                                             cAAATGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAACATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCa            >  1:610498/1‑140 (MQ=255)                                                                                                                              aaaTGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGATCCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCGCGACAGAGGAATCTTGCCGTAGGCAg           <  2:165333/140‑1 (MQ=255)                                                                                                                              aaaTGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAg           <  2:34074/140‑1 (MQ=255)                                                                                                                              aaaTGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAg           <  2:114739/140‑1 (MQ=255)                                                                                                                                aTGACGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCg         <  2:207405/140‑1 (MQ=255)                                                                                                                                   aCGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGAt      <  1:267749/140‑1 (MQ=255)                                                                                                                                   aCGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGAt      <  2:558253/140‑1 (MQ=255)                                                                                                                                   aCGGAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAACATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGAt      <  2:610498/140‑1 (MQ=255)                                                                                                                                      gAACTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGATCAg   >  1:526215/1‑140 (MQ=255)                                                                                                                                       aaCTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGATCAGc  <  1:287223/140‑1 (MQ=255)                                                                                                                                       aaCTGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGATCAGc  <  2:59326/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                         TGCTGATTTAGATATTAATTGGGTGAACTCTTCTGCCATAATTTGTTCATAAGTGCTATTGTCAGTAATTCGTGGATGCCTATTACTACAAATTAGAGTATTGCGAATCGTATGCAATGGCATGCAAATGACGGAACGGCTGATCACTGGCATTTGGGTGATCACCAGATCGGCTTTGCGGTGAACCAACAGTTCTTCAGCATTCTCTGCTGACATGAGGATATCATGGCACTCAATCTCGACAGAGGAATCTTGCCGTAGGCAGCGGATCAGC  >  NC_000913/637001‑637274

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑