Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,406,078	T→C	A54A (GCT→GCC)	accC →	biotin carboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,406,078	0	T	C	100.0%	168.8 / NA	44	A54A (GCT→GCC)	accC	biotin carboxylase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (23/21);  total (23/21)

CCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCT  >  NC_000913/3405943‑3406214                                                                                                                                        |                                                                                                                                         ccGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAACCGGTCTGTATTGGCCCTGCCCCGt                                                                                                                                      <  2:535276/140‑1 (MQ=255) ccGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGt                                                                                                                                      >  1:238304/1‑140 (MQ=255)    cggcgAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAg                                                                                                                                   >  2:442625/1‑140 (MQ=255)      gcgAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTa                                                                                                                                 >  2:613383/1‑140 (MQ=255)       cgAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTaa                                                                                                                                >  2:691272/1‑140 (MQ=255)                       aTTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGTTGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACAt                                                                                                                <  2:366063/140‑1 (MQ=255)                       aTTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGaa                                                                                                                   >  1:668397/1‑137 (MQ=255)                       aTTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGaa                                                                                                                   <  2:668397/137‑1 (MQ=255)                         tcttcGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATcc                                                                                                              <  2:238304/140‑1 (MQ=255)                            tcGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCgg                                                                                                           <  2:638186/140‑1 (MQ=255)                              gTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTa                                                                                                                         >  2:503322/1‑124 (MQ=255)                              gTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTa                                                                                                                         <  1:503322/124‑1 (MQ=255)                              gTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCa                                                                                                         >  2:635397/1‑140 (MQ=255)                                gCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAat                                                                                                       >  1:612963/1‑140 (MQ=255)                                   tGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAatcat                                                                                                    >  1:208055/1‑140 (MQ=255)                                     tAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAAtcatca                                                                                                  >  1:622068/1‑140 (MQ=255)                                      aaaGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAg                                                                                                 <  1:640083/140‑1 (MQ=255)                                        aGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAgcg                                                                                               <  2:622068/140‑1 (MQ=255)                                             tGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCt                                                                                          >  2:143324/1‑140 (MQ=255)                                               ggCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGa                                                                                        >  2:632510/1‑140 (MQ=255)                                                     aaGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCAc                                                                                  <  2:208055/140‑1 (MQ=255)                                                     aaGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCAc                                                                                  <  1:143324/140‑1 (MQ=255)                                                           gTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGgcgc                                                                            <  1:90200/140‑1 (MQ=255)                                                                  tgCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCa                                                                     <  1:691272/140‑1 (MQ=255)                                                                       tCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAatcca                                                                >  1:291705/1‑140 (MQ=255)                                                                           gcgcGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCg                                                            >  1:322617/1‑140 (MQ=255)                                                                                 aTCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTAc                                                      <  2:322617/140‑1 (MQ=255)                                                                                      gATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCtt                                                 <  2:485020/140‑1 (MQ=255)                                                                                                  gTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGaa                                     >  1:114679/1‑140 (MQ=255)                                                                                                        cTGGCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCaa                               >  1:607291/1‑140 (MQ=255)                                                                                                           gCAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACtt                            >  1:200805/1‑140 (MQ=255)                                                                                                            cAGATGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACttt                           >  2:285910/1‑140 (MQ=255)                                                                                                               aTGAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGcc                        >  2:695639/1‑140 (MQ=255)                                                                                                                 gAAACGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGa                      <  1:635110/140‑1 (MQ=255)                                                                                                                  aaaCGGTCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAg                     >  2:464710/1‑140 (MQ=255)                                                                                                                        tCTGTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGtt               >  1:510828/1‑140 (MQ=255)                                                                                                                           gTATTGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGaa            <  1:635397/140‑1 (MQ=255)                                                                                                                               tGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCt        <  2:666173/140‑1 (MQ=255)                                                                                                                               tGGCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCt        <  1:464710/140‑1 (MQ=255)                                                                                                                                 gCCCTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATTACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTcc      >  1:138369/1‑140 (MQ=255)                                                                                                                                  cccTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCg     <  2:291705/140‑1 (MQ=255)                                                                                                                                    cTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGc   <  2:283593/140‑1 (MQ=255)                                                                                                                                    cTGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGc   >  1:389465/1‑140 (MQ=255)                                                                                                                                     tGCCCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCt  <  1:613383/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                         CCGCGGCGAGATTGCATTGCGTATTCTTCGTGCCTGTAAAGAACTGGGCATCAAGACTGTCGCTGTGCACTCCAGCGCGGATCGCGATCTAAAACACGTATTACTGGCAGATGAAACGGTCTGTATTGGCCCTGCTCCGTCAGTAAAAAGTTATCTGAACATCCCGGCAATCATCAGCGCCGCTGAAATCACCGGCGCAGTAGCAATCCATCCGGGTTACGGCTTCCTCTCCGAGAACGCCAACTTTGCCGAGCAGGTTGAACGCTCCGGCT  >  NC_000913/3405943‑3406214

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑