Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,555,972	C→G	A117P (GCG→CCG)	yjiC ←	uncharacterized protein YjiC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,555,972	0	C	G	100.0%	268.6 / NA	68	A117P (GCG→CCG)	yjiC	uncharacterized protein YjiC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (33/35);  total (33/35)

GTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTC  >  NC_000913/4555836‑4556107                                                                                                                                         |                                                                                                                                        gTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCtt                                                                                                                                      >  1:92625/1‑140 (MQ=255)       gCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACa                                                                                                                                >  1:437078/1‑140 (MQ=255)         aTAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACata                                                                                                                              <  1:185610/140‑1 (MQ=255)           aaCCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATg                                                                                                                            <  1:316168/140‑1 (MQ=255)           aaCCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATg                                                                                                                            <  2:437078/140‑1 (MQ=255)                ggAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGccacc                                                                                                                       <  2:187817/140‑1 (MQ=255)                       tGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATAtt                                                                                                                >  2:44317/1‑140 (MQ=255)                               gggCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTaa                                                                                                        >  2:207099/1‑140 (MQ=255)                                           aTTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGAt                                                                                            >  2:29391/1‑140 (MQ=255)                                                 tGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATAATTCGTCTAAAGCTGAGGTGATTTTCaa                                                                                      >  2:321262/1‑140 (MQ=255)                                                  gAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTATATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAg                                                                                     <  2:92625/140‑1 (MQ=255)                                                        tataGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGa                                                                               >  1:444062/1‑140 (MQ=255)                                                        tataGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGa                                                                               >  2:467662/1‑140 (MQ=255)                                                        tataGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGa                                                                               >  1:633500/1‑140 (MQ=255)                                                        tataGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGa                                                                               >  2:489737/1‑140 (MQ=255)                                                        tataGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATAATTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGa                                                                               <  1:321262/140‑1 (MQ=255)                                                              ccTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGc                                                                         >  2:503737/1‑140 (MQ=255)                                                               cTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCa                                                                        >  2:480688/1‑140 (MQ=255)                                                                tCAACAGGATCTAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCAt                                                                       >  1:33884/1‑140 (MQ=255)                                                                tCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATa                                                                             <  2:489636/134‑1 (MQ=255)                                                                tCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATa                                                                             >  1:489636/1‑134 (MQ=255)                                                                tCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCAt                                                                       >  1:497816/1‑140 (MQ=255)                                                                tCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCAt                                                                       <  1:489737/140‑1 (MQ=255)                                                                tCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCAt                                                                       <  1:29391/140‑1 (MQ=255)                                                                tCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCAt                                                                       >  1:648746/1‑140 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATa                                                                             >  1:182056/1‑132 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATa                                                                             <  2:182056/132‑1 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATAc                                                                     >  2:241023/1‑140 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATAc                                                                     >  1:122206/1‑140 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATAc                                                                     >  1:113854/1‑140 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATAc                                                                     >  1:681525/1‑140 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATAc                                                                     >  1:681350/1‑140 (MQ=255)                                                                  aaCAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATAc                                                                     >  1:659273/1‑140 (MQ=255)                                                                     aGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTc                                                                  <  2:10622/140‑1 (MQ=255)                                                                     aGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTc                                                                  <  1:207099/140‑1 (MQ=255)                                                                     aGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTc                                                                  <  2:430365/140‑1 (MQ=255)                                                                       gATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCt                                                                <  2:355598/140‑1 (MQ=255)                                                                         tCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCtgt                                                              <  1:480688/140‑1 (MQ=255)                                                                         tCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCtgt                                                              <  2:497816/140‑1 (MQ=255)                                                                         tCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCtgt                                                              <  1:644866/140‑1 (MQ=255)                                                                         tCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCtgt                                                              <  2:14138/140‑1 (MQ=255)                                                                         tCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCtgt                                                              <  2:122206/140‑1 (MQ=255)                                                                               gggCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCtgttgttgt                                                        >  2:45670/1‑140 (MQ=255)                                                                                     ttCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATAcc                                                  >  2:70121/1‑140 (MQ=255)                                                                                     ttCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATAcc                                                  >  2:342509/1‑140 (MQ=255)                                                                                            ataaatTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGAt                                           <  2:681525/140‑1 (MQ=255)                                                                                            ataaatTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGAt                                           >  2:532350/1‑140 (MQ=255)                                                                                               aatTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGAtt                                          >  1:57489/1‑138 (MQ=255)                                                                                               aatTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGAtt                                          <  2:57489/138‑1 (MQ=255)                                                                                                   tAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGc                                    <  2:491695/140‑1 (MQ=255)                                                                                                      ccGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATg                                 <  2:356583/140‑1 (MQ=255)                                                                                                            aCCTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTg                           <  2:444062/140‑1 (MQ=255)                                                                                                              cTTCTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTc                         <  2:33884/140‑1 (MQ=255)                                                                                                                 cTGCTAACTCTGTGGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTcaca                      <  1:70121/140‑1 (MQ=255)                                                                                                                            gtgGTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATgg           >  2:687272/1‑140 (MQ=255)                                                                                                                               gTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCa        <  1:302885/140‑1 (MQ=255)                                                                                                                               gTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCa        <  2:399122/140‑1 (MQ=255)                                                                                                                               gTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCa        <  2:648746/140‑1 (MQ=255)                                                                                                                               gTAGCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCa        <  2:349114/140‑1 (MQ=255)                                                                                                                                  gCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAgg     <  1:467662/140‑1 (MQ=255)                                                                                                                                  gCATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAgg     <  1:503737/140‑1 (MQ=255)                                                                                                                                   cATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAgg     <  1:463512/139‑1 (MQ=255)                                                                                                                                   cATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAgg     >  2:463512/1‑139 (MQ=255)                                                                                                                                   cATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGc    >  1:93938/1‑140 (MQ=255)                                                                                                                                   cATGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGc    >  2:654587/1‑140 (MQ=255)                                                                                                                                     tGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAgg     <  1:562714/137‑1 (MQ=255)                                                                                                                                     tGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAgg     >  2:562714/1‑137 (MQ=255)                                                                                                                                     tGCGGCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGctc  <  2:93938/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                        GTTCCGGCATAACCAGGAACATTGCAACCAGGGCCTAATGTTATTTCATGAACTGTATAGTCCTCAACAGGATCGAAAGGGCTCTTCATAAATAAATTTAGCCGTAAACCTTCTGCTAACTCTGTGGTAGCATGCGCCTTTTTACATATGCCACCATATATTTCGTCTAAAGCTGAGGTGATTTTCAAGTTCCGATAGAGCATACGTCCTGTTGTTGTAATACCATCGGATTCTCTGCATGCTCCTGTCACATAATTAAATGGCCAAGGCTC  >  NC_000913/4555836‑4556107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑