Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,723,518	(G)5→6	coding (1398/2013 nt)	ydhK →	putative transporter YdhK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,723,513	1	.	G	100.0%	97.6 / NA	26	W465G (TGG→GGG)	ydhK	putative transporter YdhK
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (13/13);  total (13/13)

CTTTCGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGT‑GGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGTTGGCG  >  NC_000913/1723375‑1723629                                                                                                                                            |                                                                                                                     cTTTCGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATtgt‑g                                                                                                                     <  2:600752/140‑1 (MQ=255)     cGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATTGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGT‑ggggg                                                                                                                 >  2:105150/1‑140 (MQ=255)     cGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGT‑ggggg                                                                                                                 >  2:275555/1‑140 (MQ=255)     cGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGT‑ggggg                                                                                                                 >  2:303416/1‑140 (MQ=255)     cGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGT‑ggggg                                                                                                                 >  1:428535/1‑140 (MQ=255)             aGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCAGCATTGTGGGGGGCAACTGa                                                                                                          <  2:733684/140‑1 (MQ=255)                    tgGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGtttt                                                                                                   >  2:381388/1‑140 (MQ=255)                         aaaTTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATgg                                                                                              <  1:275555/140‑1 (MQ=255)                          aaTTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATggg                                                                                             <  2:600807/140‑1 (MQ=255)                                 tCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCGCTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCtttt                                                                                      >  1:195149/1‑140 (MQ=255)                                  cTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTa                                                                                     <  2:723116/140‑1 (MQ=255)                                  cTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTa                                                                                     <  1:248277/140‑1 (MQ=255)                                     aTGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCg                                                                                  >  2:677252/1‑140 (MQ=255)                                          ccAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTc                                                                             >  1:155285/1‑140 (MQ=255)                                            aGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCAc                                                                           <  1:677252/140‑1 (MQ=255)                                                   cGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATccc                                                                    >  1:118062/1‑140 (MQ=255)                                                   cGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATccc                                                                    >  2:227885/1‑140 (MQ=255)                                                    gATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATcccc                                                                   <  1:105150/140‑1 (MQ=255)                                                    gATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATcccc                                                                   <  2:118062/140‑1 (MQ=255)                                                    gATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATcccc                                                                   <  1:567902/140‑1 (MQ=255)                                                     aTCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATccccc                                                                  <  2:356079/140‑1 (MQ=255)                                                      tCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCg                                                                 <  2:428535/140‑1 (MQ=255)                                                       cTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCgg                                                                >  2:521131/1‑140 (MQ=255)                                                                tttttACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGAtt                                                       >  1:212287/1‑140 (MQ=255)                                                                         tttCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGAtt                                              <  1:112149/140‑1 (MQ=255)                                                                          ttCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGAttt                                             >  2:133057/1‑140 (MQ=255)                                                                            ctctTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTc                                           <  1:381388/140‑1 (MQ=255)                                                                                                  tGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAAt                     >  2:310425/1‑140 (MQ=255)                                                                                                                 tGCAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGtt      >  2:558790/1‑140 (MQ=255)                                                                                                                   cAGATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGTTgg    >  2:176330/1‑140 (MQ=255)                                                                                                                     gATGCCAAAATTTGCCGCATTGTGGGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGTTGGCg  >  1:156844/1‑140 (MQ=255)                                                                                                                                            |                                                                                                                     CTTTCGCTATTCAGCTTTGTGGTCAAATTTGGTCTGATGGTCCAGATTAGCGATCTGTGGCAATTTTTACTGTTTCTCTTTCCACTGCTGGCGACAATGCAGCTTCTTAAATTGCAGATGCCAAAATTTGCCGCATTGT‑GGGGGCAACTGATTGTTTTTATGGGTTCTTTTATCGCTGTCACTAATCCCCCGGTGTATGATTTTGCTGATTTTCTTAACGATAATCTGGCAAAAATCGTTGGCGTCGCGTTGGCG  >  NC_000913/1723375‑1723629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑