Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,298,647	C→A	A24A (GCG→GCT)	napB ←	periplasmic nitrate reductase cytochrome c550 protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,298,647	0	C	A	100.0%	80.4 / NA	24	A24A (GCG→GCT)	napB	periplasmic nitrate reductase cytochrome c550 protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (9/15);  total (9/15)

ATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACA  >  NC_000913/2298512‑2298771                                                                                                                                        |                                                                                                                             aTGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAgcgc                                                                                                                          <  2:414339/140‑1 (MQ=255) aTGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAgcgc                                                                                                                          <  1:50688/140‑1 (MQ=255)  tGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGcgcc                                                                                                                         >  1:322722/1‑140 (MQ=255)            ttGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTaccacc                                                                                                               >  1:121586/1‑140 (MQ=255)                         cacaTAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCg                                                                                                  <  2:121586/140‑1 (MQ=255)                              aGTTTAGCGGCATCCGATCCTGCTCTTTTGGCAACCGAATGGCCCCTTCCTGGGGCCCGGATACTTCCGGCGACTGGCTAAAATCGGCGCCCTTAGCCCCCCAAACAGGGCCGGTTTCCCCCAGGGCCAGCATCGCCGTc                                                                                             <  2:291407/140‑1 (MQ=255)                                ttCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCa                                                                                           >  2:34364/1‑140 (MQ=255)                                  cAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCAtt                                                                                         >  2:513270/1‑140 (MQ=255)                                      ggCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCTACGCCCTTAGCCGCACAAACAGCTCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGaca                                                                                     >  2:80244/1‑140 (MQ=255)                                          tCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAgc                                                                                 >  1:603649/1‑140 (MQ=255)                                           ccGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAgcg                                                                                <  1:34364/140‑1 (MQ=255)                                                              aTCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCt                                                             >  1:617822/1‑140 (MQ=255)                                                              aTCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCt                                                             >  2:723386/1‑140 (MQ=255)                                                                   aaTGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCa                                                        >  1:528546/1‑140 (MQ=255)                                                                              ccTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTc                                             <  2:139242/140‑1 (MQ=255)                                                                              ccTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTc                                             <  1:443194/140‑1 (MQ=255)                                                                                     cccGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACt                                      <  2:187340/140‑1 (MQ=255)                                                                                        ggATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCa                                   <  2:617822/140‑1 (MQ=255)                                                                                            aCTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCgt                               <  1:723386/140‑1 (MQ=255)                                                                                                ccGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAg                           <  2:528546/140‑1 (MQ=255)                                                                                                ccGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAg                           <  2:391405/140‑1 (MQ=255)                                                                                                             aaaaTCGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATAc              <  1:513270/140‑1 (MQ=255)                                                                                                                  cGACGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCt         <  2:460117/140‑1 (MQ=255)                                                                                                                    aCGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCt         >  2:296502/1‑138 (MQ=255)                                                                                                                    aCGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCt         <  1:296502/138‑1 (MQ=255)                                                                                                                    aCGCCATTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCa       >  2:109427/1‑140 (MQ=255)                                                                                                                         aTTAGCCGCCCAAACAGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACa  <  1:577807/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                             ATGCGGGATCATTGGCGGCTGATTCACATAGTTCAGCGGCATCCGATCCTGCTCTTTTGGCATCCGAATGGCCCCTTCCTGTGTCCCGGATACTTCCGGCGACTGGCTAAAATCGACGCCATTAGCCGCCCAAACCGCGCCGCTTACCACCAGGGCCAGCATCGCCGTCCATTGACACAGCGCTTTCTTCAGGTCATGGCTTTTCATGATTTCGCTCCCGAACTCCATCGTGTAGTTATTGTAAATACATCCTCAGAACA  >  NC_000913/2298512‑2298771

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑