| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| JC | NC_000913 | 1,367,889 | (CAAGT)1→2 | coding (24/978 nt) | pspF ← | DNA‑binding transcriptional dual regulator PspF |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 1367884 | 0 (0.000) | 47 (0.900) +CAAGT |
37/256 | 0.2 | 100% | coding (29/978 nt) | pspF | DNA‑binding transcriptional dual regulator PspF |
| ? | NC_000913 | 1367885 = | 0 (0.000) | coding (28/978 nt) | pspF | DNA‑binding transcriptional dual regulator PspF | |||||
AACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTCACCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCAA > NC_000913/1367747‑1368021 | gaggagagataatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtca > 1:601844‑M2/139‑140 (MQ=255)gaggagagataatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtca < 1:124495‑M2/2‑1 (MQ=255) agataatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTaaa < 1:617175‑M2/8‑1 (MQ=255) gataatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAAt < 2:601844‑M2/9‑1 (MQ=255) gataatgcaggcggctggcaatcagctcagtaccagtgccgcgttcgacgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgactcaccaagtCAAGTAAAt < 2:425099‑M2/9‑1 (MQ=255) ataatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAAtt > 2:437736‑M2/131‑140 (MQ=255) ataatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAAtt < 2:749674‑M2/10‑1 (MQ=255) taatgcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTa < 2:449974‑M2/11‑1 (MQ=255) gcaggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCtt > 2:377186‑M2/126‑140 (MQ=255) caggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCttt < 2:579458‑M2/16‑1 (MQ=255) caggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCttt < 1:437736‑M2/16‑1 (MQ=255) ggcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGt > 2:458386‑M2/123‑140 (MQ=255) gcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTa > 2:826615‑M2/122‑140 (MQ=255) gcggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTa > 2:622568‑M2/122‑140 (MQ=255) cggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtcaa < 2:272612‑M2/3‑1 (MQ=255) cggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtcaa > 1:272612‑M2/121‑123 (MQ=255) cggctggcaatcagctctttaccggtgccgcggtcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTAt > 1:353657‑M2/121‑140 (MQ=255) ggctggcaatcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTAtt < 2:480493‑M2/21‑1 (MQ=255) atcagctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCatg > 2:636192‑M2/112‑140 (MQ=255) gctctttaccggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCAtgatga > 1:12473‑M2/108‑140 (MQ=255) cggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCa < 1:622568‑M2/42‑1 (MQ=255) cggtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCa < 2:356459‑M2/42‑1 (MQ=255) gtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCatga < 1:570993‑M2/30‑1 (MQ=255) gtgccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCatga > 2:570993‑M2/97‑126 (MQ=255) ccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGt < 2:461477‑M2/47‑1 (MQ=255) ccgcgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGt < 1:400317‑M2/47‑1 (MQ=255) cgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAg > 1:447746‑M2/91‑140 (MQ=255) cgttcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAg > 1:13672‑M2/91‑140 (MQ=255) tcgccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAgtgt < 1:458386‑M2/53‑1 (MQ=255) gccgatgatgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTaa < 1:856190‑M2/55‑1 (MQ=255) tgagcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTaa < 1:636192‑M2/63‑1 (MQ=255) agcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACt > 1:683924‑M2/76‑140 (MQ=255) agcaccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACt < 2:447746‑M2/65‑1 (MQ=255) ccggtttgtccagcggtgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATc < 1:826615‑M2/69‑1 (MQ=255) tgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGtt > 2:599924‑M2/56‑140 (MQ=255) tgcgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGtt > 2:717577‑M2/56‑140 (MQ=255) cgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGa > 1:290906‑M2/54‑140 (MQ=255) cgagatgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGa > 1:38517‑M2/54‑140 (MQ=255) atgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGAttct < 2:290906‑M2/91‑1 (MQ=255) atgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGAttct < 1:612366‑M2/91‑1 (MQ=255) tgcgaaacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGAttctt > 2:422929‑M2/49‑140 (MQ=255) aacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAAt > 2:412028‑M2/44‑139 (MQ=255) aacctgttccagcacttcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAAt < 1:412028‑M2/96‑1 (MQ=255) tcgagaaagctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCa > 2:789380‑M2/28‑140 (MQ=255) ctgttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATaa > 2:669229‑M2/19‑140 (MQ=255) ttcgcctcaccaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATaaaaa > 2:455918‑M2/16‑140 (MQ=255) accaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCAc > 1:38408‑M2/8‑140 (MQ=255) accaagtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCAc > 2:909880‑M2/8‑140 (MQ=255) agtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCaa < 1:909880‑M2/137‑1 (MQ=255) agtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCaa < 1:536437‑M2/137‑1 (MQ=255) agtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCaa < 2:683924‑M2/137‑1 (MQ=255) agtCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCaa < 1:422929‑M2/137‑1 (MQ=255) | AACGGGAGGAGAGATAATGCAGGCGGCTGGCAATCAGCTCTTTACCGGTGCCGCGTTCGCCGATGATGAGCACCGGTTTGTCCAGCGGTGCGAGATGCGAAACCTGTTCCAGCACTTCGAGAAAGCTGTTCGCCTCACCAAGTAAATTATCTTTGTATTCTGCCATGATGAAATTCGCCACTTGTTAGTGTAATTCGCTAACTCATCCTGGCATGTTGCTGTTGATTCTTCAATCAGATCTTTATAAATCAAAAAGATAAAAAATTGGCACGCAA > NC_000913/1367747‑1368021 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |