Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913332,9910GT20.8% 135.1 / 13.4 53L207F (TTG→TTT) pdeLDNA‑binding transcriptional activator/c‑di‑GMP phosphodiesterase PdeL
Reads supporting (aligned to +/- strand):  ref base G (15/27);  new base T (11/0);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 1.01e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAG  >  NC_000913/332855‑333109
                                                                                                                                        |                                                                                                                      
tGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCa                                                                                                                     >  1:734311/1‑140 (MQ=255)
       gTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGTATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGAt                                                                                                              >  2:121131/1‑140 (MQ=255)
       gTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGTATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGAt                                                                                                              <  1:121131/140‑1 (MQ=255)
                tCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTGaaaaaa                                                                                                     >  2:521523/1‑139 (MQ=255)
                 cTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAaga                                                                                                    <  2:734311/140‑1 (MQ=255)
                 cTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAaga                                                                                                    <  2:226082/140‑1 (MQ=255)
                  ttATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAagag                                                                                                   >  1:72731/1‑140 (MQ=255)
                      tGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTc                                                                                               <  2:332460/140‑1 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGATTTGAAAAAGAGTGTCTGaa                                                                                           >  2:592998/1‑140 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGaaaaaaa                                                                                                    >  1:584176/1‑129 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAaga                                                                                                    <  2:584176/131‑1 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGGCGCGGGGTTTTGAAAAAAGTGTCTgga                                                                                           >  2:877288/1‑138 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTTAAAAAAAGGGTCTGaa                                                                                           >  2:181484/1‑140 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGATTTGAAAAAGAGGGTCTGaa                                                                                           >  1:506893/1‑140 (MQ=255)
                          aTAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGATGGTTTTTTGCTGGGGGACTTGAAAAAAAgtgatggaa                                                                                           >  2:807367/1‑134 (MQ=255)
                           tAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAAc                                                                                          <  2:411924/140‑1 (MQ=255)
                           tAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAAc                                                                                          <  1:521523/140‑1 (MQ=255)
                             aTGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTTAAAAAGAGGGTCTGAACCt                                                                                        >  1:143162/1‑140 (MQ=255)
                              tGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTGAAAAAGAGGGGCTGGACCTg                                                                                       >  2:90163/1‑140 (MQ=255)
                              cagcccGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTg                                                                                       <  1:697562/137‑1 (MQ=255)
                                aCCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGt                                                                                     <  2:843196/140‑1 (MQ=255)
                                aCCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGt                                                                                     <  1:389388/140‑1 (MQ=255)
                                 cccGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGtt                                                                                    <  2:15510/140‑1 (MQ=255)
                                  ccGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTta                                                                                   >  1:195025/1‑140 (MQ=255)
                                  ccGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTta                                                                                   <  1:877288/140‑1 (MQ=255)
                                  ccGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTta                                                                                   <  2:72731/140‑1 (MQ=255)
                                  ccGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTGAAAAAGAGGGGCTGAACCTGGTta                                                                                   >  2:194110/1‑140 (MQ=255)
                                    gCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGGTTTTAAAAAGAGTGTCTTAACCTTGTtaat                                                                                 >  2:362290/1‑140 (MQ=255)
                                      cAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGATTTTAAAAAAAGGGTCTGAACCTGGTtaataa                                                                               >  2:339488/1‑140 (MQ=255)
                                            atgaAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGGTTTTGAAAAGAGTGTCTTAAACTGGTTAATAAAttttt                                                                         >  2:697562/1‑137 (MQ=255)
                                             tgaAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGt                                                                        <  1:697526/140‑1 (MQ=255)
                                              gaAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTa                                                                       <  1:90163/140‑1 (MQ=255)
                                                   aGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTTTTTTTTTGTCGGGGGGGTTTAAAAAGGGGGTTTTTACCTGGGTAATAAAATAGGTAACGaa                                                                  >  2:727653/1‑139 (MQ=255)
                                                        gCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAAt                                                             <  1:249243/140‑1 (MQ=255)
                                                              atTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCt                                                       <  2:764469/140‑1 (MQ=255)
                                                                   gATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAAAGTGTCTGAACCTGGTTAATAAATTAGGGAACGATAAAATCAAGCTGGttt                                                  >  1:226382/1‑139 (MQ=255)
                                                                     tGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGGTTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGGAACGATAAAATCAAGCTGGTtctc                                                >  1:694910/1‑140 (MQ=255)
                                                                         ggTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGGCGCGGGATTTTAAAAAGAGAGTCTCAAACCGGGTAATTAAATAGGTAACAATAAAATCAAGCTGGTTTTCGaac                                            >  2:263624/1‑138 (MQ=255)
                                                                         ggTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAAACTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGc                                            >  1:103749/1‑140 (MQ=255)
                                                                         ggTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGGTTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGc                                            >  2:142058/1‑140 (MQ=255)
                                                                          gTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGGTGTTTTTTTGCAGCGGGATTTGAAAAAAAGGGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAACCGGTTCTCCAGCt                                           >  2:584727/1‑140 (MQ=255)
                                                                                        gcCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAAcc                             <  1:181484/140‑1 (MQ=255)
                                                                                        gcCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAAcc                             <  1:592998/140‑1 (MQ=255)
                                                                                                  ttCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGt                   <  1:362290/140‑1 (MQ=255)
                                                                                                    ccATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTaa                 <  1:194110/140‑1 (MQ=255)
                                                                                                         ttGGCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCa            >  1:232974/1‑140 (MQ=255)
                                                                                                         ttGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCa            <  1:339488/140‑1 (MQ=255)
                                                                                                           ggCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGa          >  1:571949/1‑140 (MQ=255)
                                                                                                           ggCATCAACGTCTCGGCGGGGTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAAACTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGa          >  1:94521/1‑140 (MQ=255)
                                                                                                            gCATCAACGTCTCGGCGGGTTGTTTTTTTGCAGCGGGGTTTGAAAAAGAGGGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGaa         >  2:564784/1‑140 (MQ=255)
                                                                                                            gCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAAAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAAACAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGaa         >  1:217490/1‑140 (MQ=255)
                                                                                                             cATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTaa                 >  1:358868/1‑131 (MQ=255)
                                                                                                             cATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTaa                 <  2:358868/131‑1 (MQ=255)
                                                                                                                cAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCa     <  1:205514/140‑1 (MQ=255)
                                                                                                                 aaCGTCTCGGCGGGTTGTTTTTTGGCAGCGGGGTTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCag    >  1:52276/1‑140 (MQ=255)
                                                                                                                   cGTCTCGGCGGGTTGTTTTTTTGCAGGGGGGTTTTAAAAAAAGGGTCTGAACCTGGGTAATAAATTAGGTAACGATAAAATTAAGCTGGTTTTCGAGGCAACGGAACGTAAACCTATTTCGGGAAAGCCAGAAGCCagag  >  2:205514/1‑140 (MQ=255)
                                                                                                                   cGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCagag  <  2:195025/140‑1 (MQ=255)
                                                                                                                   cGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCagag  <  1:263624/140‑1 (MQ=255)
                                                                                                                   cGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCagag  <  1:537617/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                      
TGGCGGAGTCATCCGGTCTTATTGTCATAATGACCCGCCAACTGATGAAACAGACTGCGGATATTCTGATGCCGGTAAAACATTTGCTGCCGGACAATTTCCATATTGGCATCAACGTCTCGGCGGGTTGTTTTTTGGCAGCGGGATTTGAAAAAGAGTGTCTGAACCTGGTTAATAAATTAGGTAACGATAAAATCAAGCTGGTTCTCGAGCTAACGGAACGTAACCCTATTCCGGTAACGCCAGAAGCCAGAG  >  NC_000913/332855‑333109

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑