Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4440TA46.9% 12.5 / 27.6 32intergenic (‑188/‑19)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
Reads supporting (aligned to +/- strand):  ref base T (2/15);  new base A (15/0);  total (17/15)
Fisher's exact test for biased strand distribution p-value = 2.42e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

TCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206318‑3206577
                                                                                                                              |                                                                                                                                     
tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                          <  2:543678/140‑1 (MQ=255)
   ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                       >  1:574219/1‑140 (MQ=255)
               attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCg                                                                                                           <  1:560555/140‑1 (MQ=255)
                ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                          <  2:865186/140‑1 (MQ=255)
                  cTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAGACAAAAAAAAAAAAAAGAGGTGTCATGATGAGGGaaa                                                                                                        >  2:225221/1‑140 (MQ=255)
                   ttATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAg                                                                                                       <  1:257768/140‑1 (MQ=255)
                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAAAAAAAAATGGGGTTGGCCTTATGAGGGAAAga                                                                                                      >  2:57688/1‑139 (MQ=255)
                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGAAGAGAGGAAga                                                                                                      >  1:47390/1‑139 (MQ=255)
                    tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGGGGAAga                                                                                                      >  2:424919/1‑139 (MQ=255)
                     aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATAGAGGTGGCATGATGAGGGAAAgaa                                                                                                     >  2:257768/1‑138 (MQ=255)
                                 aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                         <  2:574219/140‑1 (MQ=255)
                                 aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                         <  1:376521/140‑1 (MQ=255)
                                           tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAGGAAAAAACAACAGGCAGTGAATaa                                                                               >  1:7412/1‑140 (MQ=255)
                                             ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGAGGTGGCATGATGAGGGGAAGAAATAAAAAAAAAGCAGGGAaaaaaa                                                                             >  2:204848/1‑138 (MQ=255)
                                             ttAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAGAAATAAAAAAAAAGCAGTGAaaaaaa                                                                             >  2:190510/1‑138 (MQ=255)
                                              tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGtt                                                                            <  1:424919/140‑1 (MQ=255)
                                                     atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGTTGGCATGATGAGCGGAAATAATAAAAAAAAGGCAGTGAAAAAATTGACagag                                                                     >  2:332322/1‑140 (MQ=255)
                                                       atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGGCATGATGAGGGGAAAGAAAAAACAAAAAGCAGTGAAAAAGATGGCAgagaa                                                                   >  2:238857/1‑139 (MQ=255)
                                                        ttCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGAtt                                                                  <  1:190510/140‑1 (MQ=255)
                                                         tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTCCACCAATAAAAAAAATGGGGTTGCCCTGTTGTGGGGAAATAAAAAGCAACAAGCAGTGAAAAAAGTGACAGAGAATg                                                                 >  2:379659/1‑140 (MQ=255)
                                                           aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAAAGGAGGTGGCATGATGAGCGAAAAAAAAAAACAAAAAGCAGTGAAAAAATTGACAGAGAATGtt                                                               >  2:109982/1‑139 (MQ=255)
                                                               tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAAAGGAGTTGGCATGATGAGCGGAAGTAAAAAAAAAAAAGCAGTGAATAAGATGACAGAGAGTGTCGCTa                                                           >  2:181506/1‑140 (MQ=255)
                                                                ggCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAACAAAAACAAAAAATGGAGTTGGCATGATGAGCGAAAGAAATAAGCAACAAGCAGAGAAAAAATTGACAAAGATTGTCGCTaa                                                          >  2:519256/1‑140 (MQ=255)
                                                                     aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                     <  1:564783/140‑1 (MQ=255)
                                                                     aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTa                                                     <  1:238857/140‑1 (MQ=255)
                                                                           taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGGGTTTCCATTATGAGGGAAAAGAAAAAACAACAAGAAGTGAATAAGTTGAAAGAAATTGTCGCTAAATTTACCGccc                                               >  2:858700/1‑139 (MQ=255)
                                                                           taAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGAGGTGTCATGATGTGGGGAAAGAAAAAACAAAAAGCAGGGAAAAAGATGACAAAGAATTTCGCTAACTTTACCGccc                                               >  2:229172/1‑139 (MQ=255)
                                                                           aaaaaCCACATTTTTAGTGGTAGTTGGGGGCGGTAAATTTTTCCAGACAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCa                                               <  2:47390/139‑1 (MQ=255)
                                                                                 cacaTTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGGAAAAAATAAACAAAAAGCAGTGAAAAAAATGACAGAGAATGTTGCTAAATTTTACGCCCTGAttt                                         >  2:564783/1‑140 (MQ=255)
                                                                                        ttAGTGGTAGTTTGTGGCGGTGATTTTTTCCAGACAAATAAAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCg                                  <  1:96659/140‑1 (MQ=255)
                                                                                          aGTGGTATTTTGTGGCGGTGAATTTTTCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGaa                                <  2:758962/140‑1 (MQ=255)
                                                                                             ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGc                             <  1:225221/140‑1 (MQ=255)
                                                                                              gTAGTTTGTGGCGGTGAATTTTTTCCCACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAGAAATAAAAAACAAGCAGGGAATAAAATGACAGAGATTGTTGCTAACTTTACCGCCCTGATTTTTACCCCAAAGcc                            >  2:85120/1‑140 (MQ=255)
                                                                                                       ggCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgt                   <  1:85120/140‑1 (MQ=255)
                                                                                                             gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGa             <  1:109982/140‑1 (MQ=255)
                                                                                                                       aGAAAAATAAAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaaa   <  1:668185/140‑1 (MQ=255)
                                                                                                                        gACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc  >  2:101408/1‑140 (MQ=255)
                                                                                                                              |                                                                                                                                     
TCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAC  >  NC_000913/3206318‑3206577

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑