Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,106,340	A→C	V296G (GTG→GGG)	wbbH ←	putative O‑antigen polymerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,106,340	0	A	C	100.0%	269.4 / NA	68	V296G (GTG→GGG)	wbbH	putative O‑antigen polymerase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (34/34);  total (34/34)

GATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGAT  >  NC_000913/2106208‑2106473                                                                                                                                     |                                                                                                                                      gATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCcacac                                                                                                                                <  2:244581/140‑1 (MQ=255)           ttCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTa                                                                                                                      <  1:59911/140‑1 (MQ=255)           ttCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTa                                                                                                                      <  1:499177/140‑1 (MQ=255)            tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAAt                                                                                                                            >  1:455480/1‑133 (MQ=255)            tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAAt                                                                                                                            <  2:455480/133‑1 (MQ=255)            tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTAt                                                                                                                     >  2:856040/1‑140 (MQ=255)            tCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTAt                                                                                                                     >  1:234493/1‑140 (MQ=255)             cGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATg                                                                                                                    <  1:602884/140‑1 (MQ=255)              gagaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGc                                                                                                                   >  2:496746/1‑140 (MQ=255)               agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCa                                                                                                                  <  1:696895/140‑1 (MQ=255)               agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCa                                                                                                                  >  2:700071/1‑140 (MQ=255)               agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCa                                                                                                                  <  2:712231/140‑1 (MQ=255)               agaCAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCa                                                                                                                  <  2:702325/140‑1 (MQ=255)               agaCAATCTCCATAAAACACCTGAAATACAGCCACGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCa                                                                                                                  <  2:100569/140‑1 (MQ=255)                      ctcCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACAt                                                                                                           <  1:496746/140‑1 (MQ=255)                       tcCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATa                                                                                                          <  2:418230/140‑1 (MQ=255)                              aaCACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTc                                                                                                   <  2:657407/140‑1 (MQ=255)                              aaCACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTc                                                                                                   <  2:902965/140‑1 (MQ=255)                                 acCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCAc                                                                                                <  2:787843/140‑1 (MQ=255)                                   cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                              >  1:582687/1‑140 (MQ=255)                                   cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                              <  2:304046/140‑1 (MQ=255)                                   cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                              >  1:295882/1‑140 (MQ=255)                                   cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                              >  1:399665/1‑140 (MQ=255)                                   cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                              >  2:472186/1‑140 (MQ=255)                                   cTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGCTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCt                                                                                              >  1:196402/1‑140 (MQ=255)                                      aaaTACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGtt                                                                                           >  2:314840/1‑140 (MQ=255)                                          aCAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATa                                                                                       <  1:472186/140‑1 (MQ=255)                                             gCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTc                                                                                                   >  1:752917/1‑125 (MQ=255)                                             gCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTc                                                                                                   <  2:752917/125‑1 (MQ=255)                                                aTGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCa                                                                                 >  1:56915/1‑140 (MQ=255)                                                 tGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATa                                                                                       <  2:447624/133‑1 (MQ=255)                                                 tGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATa                                                                                       >  1:447624/1‑133 (MQ=255)                                                        ccATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCtt                                                                         >  2:167730/1‑140 (MQ=255)                                                              tcaAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCaaaa                                                                   <  2:422075/140‑1 (MQ=255)                                                              tcaAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCaaaa                                                                   <  2:503032/140‑1 (MQ=255)                                                                  aTAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAAcc                                                               >  1:464840/1‑140 (MQ=255)                                                                   tagcttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCa                                                              <  2:295882/140‑1 (MQ=255)                                                                    agcttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaa                                                             <  2:464840/140‑1 (MQ=255)                                                                      cttagctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCaaaa                                                           >  2:361796/1‑140 (MQ=255)                                                                         agctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGAc                                                        >  1:46953/1‑140 (MQ=255)                                                                           ctCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACAt                                                      <  2:234493/140‑1 (MQ=255)                                                                               gcgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATgaga                                                  >  2:408269/1‑140 (MQ=255)                                                                               gcgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATgaga                                                  >  2:778150/1‑140 (MQ=255)                                                                                cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                                 >  1:227128/1‑140 (MQ=255)                                                                                cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                                 >  2:739549/1‑140 (MQ=255)                                                                                cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                                 >  2:610632/1‑140 (MQ=255)                                                                                cgGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGaa                                                 >  1:825670/1‑140 (MQ=255)                                                                                    aaTATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTgg                                             <  2:399665/140‑1 (MQ=255)                                                                                      tataAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCCCCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCa                                           <  2:196402/140‑1 (MQ=255)                                                                                      tataAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCa                                           <  2:582687/140‑1 (MQ=255)                                                                                      tataAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCa                                           <  1:361796/140‑1 (MQ=255)                                                                                          aaCATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTgg                                             >  2:559842/1‑134 (MQ=255)                                                                                          aaCATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTgg                                             <  1:559842/134‑1 (MQ=255)                                                                                                aTCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCag                                          >  1:857064/1‑131 (MQ=255)                                                                                                aTCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCag                                          <  2:857064/131‑1 (MQ=255)                                                                                                 tCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATAc                                >  2:33142/1‑140 (MQ=255)                                                                                                 tCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATAc                                >  2:178579/1‑140 (MQ=255)                                                                                                     aaaaaGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGc                            >  1:676662/1‑140 (MQ=255)                                                                                                      aaaaGCAGTATAAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCt                           <  1:314840/140‑1 (MQ=255)                                                                                                              tataAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATaa                   <  1:33142/140‑1 (MQ=255)                                                                                                                taAACATTTGTTGGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATaaaa                 <  2:46953/140‑1 (MQ=255)                                                                                                                        tgttgGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGaa         >  1:732215/1‑140 (MQ=255)                                                                                                                        tgttgGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGaa         >  2:770759/1‑140 (MQ=255)                                                                                                                        tgttgGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGaa         >  2:49402/1‑140 (MQ=255)                                                                                                                        tgttgGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGaa         >  2:119639/1‑140 (MQ=255)                                                                                                                          ttgGCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAc       <  1:49402/140‑1 (MQ=255)                                                                                                                            ggCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAcgc     <  1:735532/140‑1 (MQ=255)                                                                                                                            ggCAAACCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAAcgc     <  1:778150/140‑1 (MQ=255)                                                                                                                               aaaCCCCCCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGAt  >  2:338771/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                      GATATGTAATTTCGAGACAATCTCCATAAAACACCTGAAATACAGCCATGAATAACCATCATCAAATAGCTTAGCTCCGCGGAAATATAAACATAATCCGAAAAAGCAGTATAAACATTTGTTGGCAAACCCACCCACACAAATTCTTTATGCAACGACATAGAGACTCCACCTGTTAATAGCCCCATCAGCCTTTCAAAAAACCAAAAGACATGAGAACTGGCAGAGTTAGATACTTGCTGAAAATAAAACTCCTGAAACGCGAT  >  NC_000913/2106208‑2106473

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑