Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,569,429	G→T	P28Q (CCG→CAG)	eutG ←	putative alcohol dehydrogenase in ethanolamine utilization

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,569,429	0	G	T	100.0%	144.5 / NA	41	P28Q (CCG→CAG)	eutG	putative alcohol dehydrogenase in ethanolamine utilization
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/21);  total (20/21)

CAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCG  >  NC_000913/2569293‑2569556                                                                                                                                         |                                                                                                                                cAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCtgtg                                                                                                                              <  2:35808/140‑1 (MQ=255) cAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCtgtg                                                                                                                              >  1:495280/1‑140 (MQ=255)              aGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGt                                                                                                                 >  2:370539/1‑140 (MQ=255)              aGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGt                                                                                                                 >  2:563217/1‑140 (MQ=255)                cccGGCGATCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGttt                                                                                                               >  2:498528/1‑140 (MQ=255)                    gcgaTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTAc                                                                                                           <  1:498528/140‑1 (MQ=255)                     cggTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAATTGTTTTTAcc                                                                                                          <  1:672811/140‑1 (MQ=255)                          aTCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTg                                                                                                     >  2:462169/1‑140 (MQ=255)                                    gATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAgg                                                                                           >  2:535486/1‑140 (MQ=255)                                      tGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGt                                                                                         >  1:740655/1‑140 (MQ=255)                                      tGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGt                                                                                         >  1:486546/1‑140 (MQ=255)                                      tGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGt                                                                                         >  1:434607/1‑140 (MQ=255)                                      tGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGt                                                                                         >  1:300789/1‑140 (MQ=255)                                           aaaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGa                                                                                    <  1:183298/140‑1 (MQ=255)                                           aaaGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGa                                                                                    <  2:470903/140‑1 (MQ=255)                                               cTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGc                                                                                <  2:495280/140‑1 (MQ=255)                                                     gCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGaaa                                                                          <  2:486546/140‑1 (MQ=255)                                                            cGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGcgcg                                                                   <  2:434607/140‑1 (MQ=255)                                                              aaCAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGGCCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGt                                                                 <  1:186297/140‑1 (MQ=255)                                                                            cccACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtttt                                                   >  1:40396/1‑140 (MQ=255)                                                                            cccACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCAtttt                                                   >  2:14388/1‑140 (MQ=255)                                                                                cgcgTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCat                                               >  1:200746/1‑140 (MQ=255)                                                                                        cgcTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGcccc                                       <  1:563217/140‑1 (MQ=255)                                                                                             gCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTg                                                  >  2:682721/1‑124 (MQ=255)                                                                                             gCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTg                                                  <  1:682721/124‑1 (MQ=255)                                                                                              cTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCt                                 <  2:740655/140‑1 (MQ=255)                                                                                              cTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCt                                 <  1:370539/140‑1 (MQ=255)                                                                                                   cGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTtctc                            <  2:300789/140‑1 (MQ=255)                                                                                                      aaCTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCg                         <  1:618286/140‑1 (MQ=255)                                                                                                        cTGCTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGcc                       <  2:179403/140‑1 (MQ=255)                                                                                                           cTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                    >  1:699749/1‑140 (MQ=255)                                                                                                           cTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                    >  1:408387/1‑140 (MQ=255)                                                                                                           cTCACCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCac                    >  1:523854/1‑140 (MQ=255)                                                                                                              aCCGAGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCactac                 <  2:200746/140‑1 (MQ=255)                                                                                                                  aGCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGc             >  1:464023/1‑140 (MQ=255)                                                                                                                   gCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCg            <  1:634954/140‑1 (MQ=255)                                                                                                                   gCCCGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCACCTCCGCTTTCTCTCGCCCACTACTGGCg            >  1:216758/1‑140 (MQ=255)                                                                                                                    cccGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGcc                       >  1:666863/1‑128 (MQ=255)                                                                                                                    cccGGACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGcc                       <  2:666863/128‑1 (MQ=255)                                                                                                                        gACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCg                         >  1:644357/1‑122 (MQ=255)                                                                                                                        gACCGCAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCg                         <  2:644357/122‑1 (MQ=255)                                                                                                                             cAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg  <  1:535486/140‑1 (MQ=255)                                                                                                                             cAAAGCGTCACCTGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCACCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCg  <  2:216758/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                CAGGCTACGCGTCAGCCCGGCGGTCATCCCTGCCTGATGCAAAAAGCTGTCTGCCATCACGAACAGATGTTTCAGCCCACGCGTTTGCGCTTGCTGTCCGCAACTGCTCACCGAGCCCGGACCGCAAAGCGTCACCGGTGGAACGCTAAATGTTTTTACCCGTTGCAGATTCAGGGTATCGAACGCCTGAAAGAGCGCGGTCTGCAATTCATTTTGCATATAGCCCCTCCGCTTTCTCTCGCCCACTACTGGCGATCGCCAGCG  >  NC_000913/2569293‑2569556

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑