Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,666,827	A→C	A340A (GCT→GCG)	hcaT ←	putative 3‑phenylpropionate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,666,827	0	A	C	100.0%	137.2 / NA	41	A340A (GCT→GCG)	hcaT	putative 3‑phenylpropionate transporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (21/20);  total (21/20)

CCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTG  >  NC_000913/2666691‑2666958                                                                                                                                         |                                                                                                                                    ccGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATcgcg                                                                                                                                  <  1:530083/140‑1 (MQ=255)    cAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATa                                                                                                                               >  1:303277/1‑140 (MQ=255)       aaTGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTg                                                                                                                            >  2:78913/1‑140 (MQ=255)                    gCATGAGGGAACAACTTTCGGGCGTAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGa                                                                                                               <  1:66914/140‑1 (MQ=255)                        gAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGTGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGc                                                                                                           <  1:775306/140‑1 (MQ=255)                          gggAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCag                                                                                                         >  1:175242/1‑140 (MQ=255)                          gggAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCag                                                                                                         >  1:386258/1‑140 (MQ=255)                             aacaacTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGt                                                                                                      >  2:540198/1‑140 (MQ=255)                                 acTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATCCTGCCGCCCATCGCGACGGCAGAGTAAAc                                                                                                  <  2:51229/140‑1 (MQ=255)                                        ggCGCAAAAACATTGCGGGAAGCCCCCCCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATTTTGATACAGAAACCCGGCAAAAACGGTCATGATCGCGATACTGCCCCCCATGGCGACGGCAGAGTAAACCGCCTGt                                                                                           <  2:565191/140‑1 (MQ=255)                                        ggCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGt                                                                                           <  2:510598/140‑1 (MQ=255)                                                aaCATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGAt                                                                                   <  2:175242/140‑1 (MQ=255)                                                       ccGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGa                                                                                 >  1:374606/1‑135 (MQ=255)                                                       ccGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGa                                                                                 <  2:374606/135‑1 (MQ=255)                                                       ccGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCg                                                                            <  1:533863/140‑1 (MQ=255)                                                       ccGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCg                                                                            >  1:18973/1‑140 (MQ=255)                                                       ccGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCg                                                                            >  2:563994/1‑140 (MQ=255)                                                            aGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAcc                                                                       >  1:767641/1‑140 (MQ=255)                                                             gcgcCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTAccc                                                                      >  2:758512/1‑140 (MQ=255)                                                                  cccaGCGCCTTTACCCAGAACCCGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                                 <  2:287651/139‑1 (MQ=255)                                                                  cccaGCGCCATTCCCCAGACCACGCCGTGGCCCAGATATTGATACAGGAACCCGGCGAAAACGGTCATGATCGCGATCCGGCCGCCCACCGCGCGGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                                 <  2:767641/139‑1 (MQ=255)                                                                  accaGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                                 <  1:78913/140‑1 (MQ=255)                                                                     aGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagc                                                              <  1:91585/140‑1 (MQ=255)                                                                       cgcCATTCCCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGagcag                                                            <  1:119621/140‑1 (MQ=255)                                                                           aTTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAat                                                        >  2:532339/1‑140 (MQ=255)                                                                                    aaCACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                                 <  1:103911/122‑1 (MQ=255)                                                                                    aaCACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCg                                                                 >  2:103911/1‑122 (MQ=255)                                                                                         gCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCCTgtctc                                          >  1:334511/1‑136 (MQ=255)                                                                                           cGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGcc                                        <  1:540198/140‑1 (MQ=255)                                                                                           cGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGcc                                        <  2:623449/140‑1 (MQ=255)                                                                                           cGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGcc                                        <  1:563994/140‑1 (MQ=255)                                                                                           cGTGGCCAAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGcc                                        <  1:663446/140‑1 (MQ=255)                                                                                              ggCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAgg                                     >  2:248915/1‑140 (MQ=255)                                                                                                 ccAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTgg                                  <  2:262359/140‑1 (MQ=255)                                                                                                   aGATATTGATACAGGAACCCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCa                                <  2:684096/140‑1 (MQ=255)                                                                                                               atgAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGt                    >  1:298349/3‑140 (MQ=255)                                                                                                               aGGAAACCGGCGAAAACGGTCATGATCGCAATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGt                    >  1:199325/1‑140 (MQ=255)                                                                                                                 gAAACCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGc                  >  2:667997/1‑140 (MQ=255)                                                                                                                  aaaCCGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGCCAGAGTAAACCGCCTGTAAACGGATCACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTTAAGGTGcc                 >  2:596726/1‑140 (MQ=255)                                                                                                                      cGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCaa             >  2:375486/1‑140 (MQ=255)                                                                                                                      cGGCGAAAACGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCaa             >  2:356058/1‑140 (MQ=255)                                                                                                                           aaaaCGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAg        >  2:1995/1‑140 (MQ=255)                                                                                                                            aaaCGGTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGa       <  1:375486/140‑1 (MQ=255)                                                                                                                                 gTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCATAATTTg  <  1:356058/140‑1 (MQ=255)                                                                                                                                 gTCATGATCGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTg  <  2:676160/140‑1 (MQ=255)                                                                                                                                 gTCATGATCGCAATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTg  <  2:199325/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                    CCGCAAAATGCTCGAATCAGCATGAGGGAACAACTTTCGGGCGCAAAAACATTGCCGGAAGCGCCACCAGCGCCATTACCCAGAACACGCCGTGGCCCAGATATTGATACAGGAAACCGGCGAAAACGGTCATGATAGCGATACTGCCGCCCATCGCGACGGCAGAGTAAACCGCCTGTAAACGGATGACTTCGCTACCCTGGCGAGCAGCAATATAACGCATGGCGGCCAGGTGGCAGACCGTGAAGGTGCCGCAATGCAGAATTTG  >  NC_000913/2666691‑2666958

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑