Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,176,917	A→C	S96S (TCT→TCG)	gatY ←	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,176,917	0	A	C	100.0%	241.6 / NA	61	S96S (TCT→TCG)	gatY	tagatose‑1,6‑bisphosphate aldolase 2 subunit GatY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (24/37);  total (24/37)

TCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCC  >  NC_000913/2176780‑2177051                                                                                                                                          |                                                                                                                                       tCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCga                                                                                                                                      >  2:1024099/1‑140 (MQ=255)    tGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCgaacg                                                                                                                                   >  1:440151/1‑140 (MQ=255)      gccgccAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCcgaacgaa                                                                                                                                 >  2:896699/1‑140 (MQ=255)        cgccAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAAcc                                                                                                                               >  2:97958/1‑140 (MQ=255)          ccAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCtt                                                                                                                             <  2:802123/140‑1 (MQ=255)                tGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGc                                                                                                                       <  2:1069883/140‑1 (MQ=255)                    ccAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGata                                                                                                                   <  1:769501/140‑1 (MQ=255)                        cTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGt                                                                                                               <  2:584509/140‑1 (MQ=255)                        cTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGt                                                                                                               <  2:842647/140‑1 (MQ=255)                           cGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCaa                                                                                                            <  2:440151/140‑1 (MQ=255)                           cGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCaa                                                                                                            <  1:520729/140‑1 (MQ=255)                                  aCGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCgt                                                                                                     >  1:368448/1‑140 (MQ=255)                                          aTCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGt                                                                                             <  1:921833/140‑1 (MQ=255)                                             aaaGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCga                                                                                          >  1:733326/1‑140 (MQ=255)                                             aaaGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCga                                                                                          <  1:805960/140‑1 (MQ=255)                                                gCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGAt                                                                                       <  2:273405/140‑1 (MQ=255)                                                 cGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATg                                                                                      <  1:1086540/140‑1 (MQ=255)                                                  gATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGa                                                                                     <  2:510541/140‑1 (MQ=255)                                                   aTGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGaa                                                                                    <  1:1024099/140‑1 (MQ=255)                                                   aTGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGaa                                                                                    >  1:77813/1‑140 (MQ=255)                                                    tGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAAt                                                                                   >  2:304451/1‑140 (MQ=255)                                                          aaaTCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAg                                                                             <  2:57237/140‑1 (MQ=255)                                                          aaaTCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAg                                                                             <  2:915688/140‑1 (MQ=255)                                                            aTCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTg                                                                           <  1:304451/140‑1 (MQ=255)                                                              ccaccaCCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGa                                                                         <  2:177675/140‑1 (MQ=255)                                                                     ctctTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGat                                                                  <  1:21487/140‑1 (MQ=255)                                                                      tctTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGata                                                                 >  1:112773/1‑140 (MQ=255)                                                                            aCCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCtt                                                           >  1:645029/1‑140 (MQ=255)                                                                            aCCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCtt                                                           >  2:1027075/1‑140 (MQ=255)                                                                               cGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGa                                                                         >  2:418118/1‑123 (MQ=255)                                                                               cGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGa                                                                         <  1:418118/123‑1 (MQ=255)                                                                                 tGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      >  2:1026885/1‑140 (MQ=255)                                                                                 tGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      >  2:40778/1‑140 (MQ=255)                                                                                 tGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      <  1:406653/140‑1 (MQ=255)                                                                                 tGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      <  2:710140/140‑1 (MQ=255)                                                                                 tGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      <  2:645029/140‑1 (MQ=255)                                                                                 tGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      >  2:691567/1‑140 (MQ=255)                                                                                  gAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTAGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCAt                                                     >  1:396070/1‑140 (MQ=255)                                                                                      tatTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATcgcg                                                 <  1:896699/140‑1 (MQ=255)                                                                                             cgcAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      >  1:49219/1‑128 (MQ=255)                                                                                             cgcAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCa                                                      <  2:49219/128‑1 (MQ=255)                                                                                             cgcAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATcgc                                                  <  2:540299/132‑1 (MQ=255)                                                                                             cgcAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATcgc                                                  >  1:540299/1‑132 (MQ=255)                                                                                               cAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAgc                                        >  1:65168/1‑140 (MQ=255)                                                                                                  aaGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTTATATTGCTTCGCCATCGCGCTGACCAGCGcc                                     >  1:16806/1‑140 (MQ=255)                                                                                                        aaTGCGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGa                               <  2:733326/140‑1 (MQ=255)                                                                                                            cGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGAtttt                           <  1:97958/140‑1 (MQ=255)                                                                                                            cGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGAtttt                           <  1:8185/140‑1 (MQ=255)                                                                                                            cGAGGCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGAtttt                           <  1:1027075/140‑1 (MQ=255)                                                                                                               ggCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTg                        >  1:110484/1‑140 (MQ=255)                                                                                                               ggCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCACTGACCAGCGCCAACAGATTTTCTg                        >  2:617654/1‑140 (MQ=255)                                                                                                                gCGTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGt                       <  1:411501/140‑1 (MQ=255)                                                                                                                  gTCAATCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTAc                     <  2:110484/140‑1 (MQ=255)                                                                                                                      aTCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGc                 <  2:112773/140‑1 (MQ=255)                                                                                                                      aTCATGACTGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGc                 <  1:40778/140‑1 (MQ=255)                                                                                                                              tGAGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCACTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTaa         <  1:617654/140‑1 (MQ=255)                                                                                                                                aGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGc                 <  1:891497/130‑1 (MQ=255)                                                                                                                                aGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGc                 >  2:891497/1‑130 (MQ=255)                                                                                                                                aGCGCACGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAAt       <  2:77813/140‑1 (MQ=255)                                                                                                                                     aCGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTAcc                    >  2:119127/1‑122 (MQ=255)                                                                                                                                     aCGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTAcc                    <  1:119127/122‑1 (MQ=255)                                                                                                                                     aCGCCCGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGcc  >  1:33403/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                       TCCTGGCCGCCAAGTTGCCCCAGCTCCGCCTCGACGCTGACATCAAAGCGATGGCAAAAATCCACCACCTCTTTGACCCGTGAAATATTTTGCGCAAAAGGCAAATGCGAGGCGTCAATCATGACTGAGCGCACGCCAGAACGAACCTTCTGAGCGATATCGTCAAATTTCGTGTGATGGTCGAGATGAATTGCCAGTGGATGGTGATATTGCTTCGCCATCGCGCTGACCAGCGCCAACAGATTTTCTGTACCAGCATGAGTAAATGTGCC  >  NC_000913/2176780‑2177051

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑